Construct: ORF TRCN0000489535
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019503.1_s317c1
- DNA Barcode:
- GCAGTAAATGTCCGGGTTTTCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAK2 (5062)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489535
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5062 | PAK2 | p21 (RAC1) activated kinase 2 | NM_002577.4 | 100% | 100% | |
| 2 | human | 5062 | PAK2 | p21 (RAC1) activated kinase 2 | XM_011512870.2 | 100% | 100% | |
| 3 | human | 5062 | PAK2 | p21 (RAC1) activated kinase 2 | XM_017006501.1 | 100% | 100% | |
| 4 | human | 646214 | LOC646214 | p21 protein (Cdc42/Rac)-act... | NR_027053.2 | 18.2% | (many diffs) | |
| 5 | mouse | 224105 | Pak2 | p21 protein (Cdc42/Rac)-act... | NM_177326.3 | 89.6% | 97.1% | (many diffs) |
| 6 | mouse | 224105 | Pak2 | p21 protein (Cdc42/Rac)-act... | XM_006522072.3 | 89.6% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 1662
- ORF length:
- 1572
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tcccgaatca tgtctgataa cggagaactg gaagataagc 121 ctccagcacc tcctgtgcga atgagcagca ccatctttag cactggaggc aaagaccctt 181 tgtcagccaa tcacagtttg aaacctttgc cctctgttcc agaagagaaa aagcccaggc 241 ataaaatcat ctccatattc tcaggcacag agaaaggaag taaaaagaaa gaaaaggaac 301 ggccagaaat ttctcctcca tctgattttg agcacaccat ccatgttggc tttgatgctg 361 ttactggaga attcactggc atgccagaac agtgggctcg attactacag acctccaata 421 tcaccaaact agagcaaaag aagaatcctc aggctgtgct ggatgtccta aagttctacg 481 actccaacac agtgaagcag aaatatctga gctttactcc tcctgagaaa gatggctttc 541 cttctggaac accagcactg aatgccaagg gaacagaagc acccgcagta gtgacagagg 601 aggaggatga tgatgaagag actgctcctc ccgttattgc cccgcgaccg gatcatacga 661 aatcaattta cacacggtct gtaattgacc ctgttcctgc accagttggt gattcacatg 721 ttgatggtgc tgccaagtct ttagacaaac agaaaaagaa gactaagatg acagatgaag 781 agattatgga gaaattaaga actatcgtga gcataggtga ccctaagaaa aaatatacaa 841 gatatgaaaa aattggacaa ggggcttctg gtacagtttt cactgctact gacgttgcac 901 tgggacagga ggttgctatc aaacaaatta atttacagaa acagccaaag aaggaactga 961 tcattaacga gattctggtg atgaaagaat tgaaaaatcc caacatcgtt aactttttgg 1021 acagttacct ggtaggagat gaattgtttg tggtcatgga ataccttgct ggggggtcac 1081 tcactgatgt ggtaacagaa acgtgcatgg atgaagcaca gattgctgct gtatgcagag 1141 agtgtttaca ggcattggag tttttacatg ctaatcaagt gatccacaga gacatcaaaa 1201 gtgacaatgt acttttggga atggaaggat ctgttaagct cactgacttt ggtttctgtg 1261 cccagatcac ccctGAGCAG AGCAAACGCA GTACCATGGT CGGAACGCCA TACTGGATGG 1321 CACCAGAGGT GGTTACACGG AAAGCTTATG GCCCTAAAGT CGACATATGG TCTCTGGGTA 1381 TCATGGCTAT TGAGATGGTA GAAGGAGAGC CTCCATACCT CAATGAAAAT CCCTTGAGGG 1441 CCTTGTACCT AATAGCAACT AATGGAACCC CAGAACTTCA GAATCCAGAG AAACTTTCCC 1501 CAATATTTCG GGATTTCTTA AATCGATGTT TGGAAATGGA TGTGGAAAAA AGGGGTTCAG 1561 CCAAAGAATT ATTACAGCAT CCTTTCCTGA AACTGGCCAA ACCGTTATCT AGCTTGACAC 1621 CACTGATCAT GGCAGCTAAA GAAGCAATGA AGAGTAACCG TAAGGGTGGG CGCGCCGACC 1681 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1741 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1801 ATATATCTTG TGGAAAGGAC GAGCAGTAAA TGTCCGGGTT TTCCCCACGC GTTAAGTCga 1861 caatcaacct ctggattaca aaatttgtga aagatt