Construct: ORF TRCN0000489549
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021695.1_s317c1
- DNA Barcode:
- GAATTTGTTGTAATTGCGTTAAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PDGFRB (5159)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489549
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_001355017.2 | 56% | 1.4% | 1_1246del |
| 2 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_001355016.2 | 50.8% | 1.3% | 1_1537del |
| 3 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_002609.4 | 47.8% | 1.2% | 1_1729del |
| 4 | mouse | 18596 | Pdgfrb | platelet derived growth fac... | NM_008809.2 | 41% | 1.3% | (many diffs) |
| 5 | mouse | 18596 | Pdgfrb | platelet derived growth fac... | NM_001146268.1 | 40.9% | 1.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 85
- ORF end:
- 220
- ORF length:
- 135
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctctgt gagctctgac ggccatgagt acatctacgt ggaccccatg cagctgccct 121 atgactccac gtgggagctg ccgcgggacc agcttgtgct gggacgcacc ctcggctctg 181 gggcctttgg gcaggtggtg gaggccacgg ctcatggcct gagccattct caggccacga 241 tgaaagtggc cgtcaagatg cttaaatcca cagcccgcag cagtgagaag caagccctta 301 tgtcggagct gaagatcatg agtcaccttg ggccccacct gaacgtggtc aacctgttgg 361 gggcctgcac caaaggagga cccatctata tcatcactga gtactgccgc tacggagacc 421 tggtggacta cctgcaccgc aacaaacaca ccttcctgca gcaccactcc gacaagcgcc 481 gcccgcccag cgcggagctc tacagcaatg ctctgcccgt tgggctcccc ctgcccagcc 541 atgtgtcctt gaccggggag agcgacggtg gctacatgga catgagcaag gacgagtcgg 601 tggactatgt gcccatgctg gacatgaaag gagacgtcaa atatgcagac atcgagtcct 661 ccaactacat ggccccttac gataactacg ttccctctgc ccctgagagg acctgccgag 721 caactttgat caacgagtct ccagtgctaa gctacatgga cctcgtgggc ttcagctacc 781 aggtggccaa tggcatggag tttctggcct ccaagaactg cgtccacaga gacctggcgg 841 ctaggaacgt gctcatctgt gaaggcaagc tggtcaagat ctgtgacttt ggcctggctc 901 gagacatcat gcgggactcg aattacatct ccaaaggcag cacctttttg cctttaaagt 961 ggatggctcc ggagagcatc ttcaacagcc tctacaccac cctgagcgac gtgtggtcct 1021 tcgggatcct gctctgggag atcttcacct tgggtggcac cccttaccca gagctgccca 1081 tgaacgagca gttctacaat gccatcaaac ggggttaccg catggcccag cctgcccatg 1141 cctccgacga gatctatgag atcatgcaga agtgctggga agagaagttt gagattcggc 1201 cccccttctc ccagctggtg ctgcttctcg agagactgtt gggcgaaggt tacaaaaaga 1261 agtaccagca ggtggatgag gagtttctga ggagtgacca cccagccatc cttcggtccc 1321 aggcccgctt gcctgggttc catggcctcc gatctcccct ggacaccagc tccgtcctct 1381 atactgccGT GCAGCCCAAT GAGGGTGACA ACGACTATAT CATCCCCCTG CCTGACCCCA 1441 AACCCGAGGT TGCTGACGAG GGCCCACTGG AGGGTTCCCC CAGCCTAGCC AGCTCCACCC 1501 TGAATGAAGT CAACACCTCC TCAACCATCT CCTGTGACAG CCCCCTGGAG CCCCAGGACG 1561 AACCAGAGCC AGAGCCCCAG CTTGAGCTCC AGGTGGAGCC GGAGCCAGAG CTGGAACAGT 1621 TGCCGGATTC GGGGTGCCCT GCGCCTCGGG CGGAAGCAGA GGATAGCTTC CTGTAGGACC 1681 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1741 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1801 ATATATCTTG TGGAAAGGAC GAGAATTTGT TGTAATTGCG TTAAACACGC GTTAAGTCga 1861 caatcaacct ctggattaca aaatttgtga aagatt