Construct: ORF TRCN0000489554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020893.1_s317c1
- DNA Barcode:
- TTGCCCCCCACTAGAATATATGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- F2R (2149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2149 | F2R | coagulation factor II throm... | NM_001992.5 | 100% | 100% | |
| 2 | human | 2149 | F2R | coagulation factor II throm... | NM_001311313.2 | 71.5% | 71.5% | 0_1ins363 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1347
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggccg cggcggctgc tgctggtggc cgcctgcttc agtctgtgcg 121 gcccgctgtt gtctgcccgc acccgggccc gcaggccaga atcaaaagca acaaatgcca 181 ccttagatcc ccggtcattt cttctcagga accccaatga taaatatgaa ccattttggg 241 aggatgagga gaaaaatgaa agtgggttaa ctgaatacag attagtctcc atcaataaaa 301 gcagtcctct tcaaaaacaa cttcctgcat tcatctcaga agatgcctcc ggatatttga 361 ccagctcctg gctgacactc tttgtcccat ctgtgtacac cggagtgttt gtagtcagcc 421 tcccactaaa catcatggcc atcgttgtgt tcatcctgaa aatgaaggtc aagaagccgg 481 cggtggtgta catgctgcac ctggccacgg cagatgtgct gtttgtgtct gtgctcccct 541 ttaagatcag ctattacttt tccggcagtg attggcagtt tgggtctgaa ttgtgtcgct 601 tcgtcactgc agcattttac tgtaacatgt acgcctctat cttgctcatg acagtcataa 661 gcattgaccg gtttctggct gtggtgtatc ccatgcagtc cctctcctgg cgtactctgg 721 gaagggcttc cttcacttgt ctggccatct gggctttggc catcgcaggg gtagtgcctc 781 tgctcctcaa ggagcaaacc atccaggtgc ccgggctcaa catcactacc tgtcatgatg 841 tgctcaatga aaccctgctc gaaggctact atgcctacta cttctcagcc ttctctgctg 901 tcttcttttt tgtgccgctg atcatttcca cggtctgtta tgtgtctatc attcgatgtc 961 ttagctcttc cgcagttgcc aaccgcagca agaagtcccg ggctttgttc ctgtcagctg 1021 ctgttttctg caTCTTCATC ATTTGCTTCG GACCCACAAA CGTCCTCCTG ATTGCGCATT 1081 ACTCATTCCT TTCTCACACT TCCACCACAG AGGCTGCCTA CTTTGCCTAC CTCCTCTGTG 1141 TCTGTGTCAG CAGCATAAGC TGCTGCATCG ACCCCCTAAT TTACTATTAC GCTTCCTCTG 1201 AGTGCCAGAG GTACGTCTAC AGTATCTTAT GCTGCAAAGA AAGTTCCGAT CCCAGCAGTT 1261 ATAACAGCAG TGGGCAGTTG ATGGCAAGTA AAATGGATAC CTGCTCTAGT AACCTGAATA 1321 ACAGCATATA CAAAAAGCTG TTAACTTAGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTGCC 1501 CCCCACTAGA ATATATGTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt