Construct: ORF TRCN0000489566
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021653.1_s317c1
- DNA Barcode:
- GTAAAACGAGTCGCGCTGTTTTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKAG3 (53632)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489566
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53632 | PRKAG3 | protein kinase AMP-activate... | NM_017431.3 | 100% | 100% | |
| 2 | human | 53632 | PRKAG3 | protein kinase AMP-activate... | XM_017004343.2 | 100% | 100% | |
| 3 | mouse | 241113 | Prkag3 | protein kinase, AMP-activat... | NM_153744.3 | 85.4% | 84.6% | (many diffs) |
| 4 | mouse | 241113 | Prkag3 | protein kinase, AMP-activat... | XM_006495988.3 | 85.4% | 84.6% | (many diffs) |
| 5 | mouse | 241113 | Prkag3 | protein kinase, AMP-activat... | XM_006495989.3 | 85.4% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1536
- ORF length:
- 1467
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagcccggg ctggagcacg cactgcgcag gaccccttcc tggagcagcc 121 ttgggggttc tgagcatcaa gagatgagct tcctagagca agaaaacagc agctcatggc 181 catcaccagc tgtgaccagc agctcagaaa gaatccgtgg gaaacggagg gccaaagcct 241 tgagatggac aaggcagaag tcggtggagg aaggggagcc accaggtcag ggggaaggtc 301 cccggtccag gccagctgct gagtccaccg ggctggaggc cacattcccc aagaccacac 361 ccttggctca agctgatcct gccggggtgg gcactccacc aacagggtgg gactgcctcc 421 cctctgactg tacagcctca gctgcaggct ccagcacaga tgatgtggag ctggccacgg 481 agttcccagc cacagaggcc tgggagtgtg agctagaagg cctgctggaa gagaggcctg 541 ccctgtgcct gtccccgcag gccccatttc ccaagctggg ctgggatgac gaactgcgga 601 aacccggcgc ccagatctac atgcgcttca tgcaggagca cacctgctac gatgccatgg 661 caactagctc caagctagtc atcttcgaca ccatgctgga gatcaagaag gccttctttg 721 ctctggtggc caacggtgtg cgggcagccc ctctatggga cagcaagaag cagagctttg 781 tggggatgct gaccatcact gacttcatcc tggtgctgca tcgctactac aggtcccccc 841 tggtccagat ctatgagatt gaacaacata agattgagac ctggagggag atctacctgc 901 aaggctgctt caagcctctg gtctccatct ctcctaatga tagcctgttt gaagctgtct 961 acaccctcat caagaaccgg atccatcgcc tgcctgttct tgacccggtg tcaggcaacg 1021 tactccacat cctcacacac aaacgcctgc tcaagttcct gcacatcttt ggttccctgc 1081 tgccccggcc ctccttcctc taccgcacta tccaagattt gggcatcggc acattccgag 1141 acttggctgt ggtgctGGAG ACAGCACCCA TCCTGACTGC ACTGGACATC TTTGTGGACC 1201 GGCGTGTGTC TGCACTGCCT GTGGTCAACG AATGTGGTCA GGTCGTGGGC CTCTATTCCC 1261 GCTTTGATGT GATTCACCTG GCTGCCCAGC AAACCTACAA CCACCTGGAC ATGAGTGTGG 1321 GAGAAGCCCT GAGGCAGAGG ACACTATGTC TGGAGGGAGT CCTTTCCTGC CAGCCCCACG 1381 AGAGCTTGGG GGAAGTGATC GACAGGATTG CTCGGGAGCA GGTACACAGG CTGGTGCTAG 1441 TGGACGAGAC CCAGCATCTC TTGGGCGTGG TCTCCCTCTC CGACATCCTT CAGGCACTGG 1501 TGCTCAGCCC TGCTGGCATC GATGCCCTCG GGGCCTAGAA CCCAGCTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGAGTAAAA CGAGTCGCGC TGTTTTCAAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt