Construct: ORF TRCN0000489575

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020406.1_s317c1
DNA Barcode:
CGTTCGGAATTCTGTGTTGTACCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
BRAF (673)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489575
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 673 BRAF B-Raf proto-oncogene, serin... NM_004333.6 100% 100%
2 human 673 BRAF B-Raf proto-oncogene, serin... NM_001354609.2 99.5% 99.3% (many diffs)
3 human 673 BRAF B-Raf proto-oncogene, serin... NM_001374244.1 95% 95% 1175_1294del
4 human 673 BRAF B-Raf proto-oncogene, serin... NM_001374258.1 94.6% 94.4% (many diffs)
5 human 673 BRAF B-Raf proto-oncogene, serin... XM_017012559.1 89.3% 87.7% (many diffs)
6 human 673 BRAF B-Raf proto-oncogene, serin... XR_001744857.1 48.1% (many diffs)
7 human 673 BRAF B-Raf proto-oncogene, serin... XR_001744858.1 22.1% (many diffs)
8 mouse 109880 Braf Braf transforming gene XM_006505359.3 82.4% 86.6% (many diffs)
9 mouse 109880 Braf Braf transforming gene XM_006505358.3 82.1% 86.2% (many diffs)
10 mouse 109880 Braf Braf transforming gene XM_011241139.2 81.8% 85.9% (many diffs)
11 mouse 109880 Braf Braf transforming gene NM_139294.5 80.9% 85% (many diffs)
12 mouse 109880 Braf Braf transforming gene XM_011241138.2 80.7% 84.7% (many diffs)
13 mouse 109880 Braf Braf transforming gene XM_011241136.2 80.7% 84.6% (many diffs)
14 mouse 109880 Braf Braf transforming gene XM_011241137.1 80.1% 84.1% (many diffs)
15 mouse 109880 Braf Braf transforming gene XM_006505355.3 78.5% 82.4% (many diffs)
16 mouse 109880 Braf Braf transforming gene XM_011241135.2 78.2% 82.1% (many diffs)
17 mouse 109880 Braf Braf transforming gene XM_006505353.3 77.5% 81.3% (many diffs)
18 mouse 109880 Braf Braf transforming gene XM_011241134.2 77.2% 81% (many diffs)
19 mouse 109880 Braf Braf transforming gene XM_011241140.2 56.8% 59.8% (many diffs)
20 mouse 109880 Braf Braf transforming gene XR_001785085.1 43.4% (many diffs)
21 mouse 109880 Braf Braf transforming gene XR_001785086.1 39.9% (many diffs)
22 mouse 109880 Braf Braf transforming gene XR_001785087.1 38.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2370
ORF length:
2298
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggcggcg ctgagcggtg gcggtggtgg cggcgcggag ccgggccagg
121ctctgttcaa cggggacatg gagcccgagg ccggcgccgg cgccggcgcc gcggcctctt
181cggctgcgga ccctgccatt ccggaggagg tgtggaatat caaacaaatg attaagttga
241cacaggaaca tatagaggcc ctattggaca aatttggtgg ggagcataat ccaccatcaa
301tatatctgga ggcctatgaa gaatacacca gcaagctaga tgcactccaa caaagagaac
361aacagttatt ggaatctctg gggaacggaa ctgatttttc tgtttctagc tctgcatcaa
421tggataccgt tacatcttct tcctcttcta gcctttcagt gctaccttca tctctttcag
481tttttcaaaa tcccacagat gtggcacgga gcaaccccaa gtcaccacaa aaacctatcg
541ttagagtctt cctgcccaac aaacagagga cagtggtacc tgcaaggtgt ggagttacag
601tccgagacag tctaaagaaa gcactgatga tgagaggtct aatcccagag tgctgtgctg
661tttacagaat tcaggatgga gagaagaaac caattggttg ggacactgat atttcctggc
721ttactggaga agaattgcat gtggaagtgt tggagaatgt tccacttaca acacacaact
781ttgtacgaaa aacgtttttc accttagcat tttgtgactt ttgtcgaaag ctgcttttcc
841agggtttccg ctgtcaaaca tgtggttata aatttcacca gcgttgtagt acagaagttc
901cactgatgtg tgttaattat gaccaacttg atttgctgtt tgtctccaag ttctttgaac
961accacccaat accacaggaa gaggcgtcct tagcagagac tgccctaaca tctggatcat
1021ccccttccgc acccgcctcg gactctattg ggccccaaat tctcaccagt ccgtctcctt
1081caaaatccat tccaattcca cagcccttcc gaccagcaga tgaagatcat cgaaatcaat
1141ttgggcaacg agaccgatcc tcatcagctc ccaatgtgca tataaacaca atagaacctg
1201tcaatattga tgacttgatt agagaccaag gatttcgtgg tgatggagga tcaaccacag
1261gtttgtctgc taccccccct gcctcattac ctggctcact aactaacgtg aaagccttac
1321agaaatctcc aggacctcag cgagaaagga agtcatcttc atcctcagaa gacaggaatc
1381gaatgaaaac acttggtaga cgggactcga gtgatgattg ggagattcct gatgggcaga
1441ttacagtggg acaaagaatt ggatctggat catttggaac agtctacaag ggaaagtggc
1501atggtgatgt ggcagtgaaa atgttgaatg tgacagcacc tacacctcag cagttacaag
1561ccttcaaaaa tgaagtagga gtactcagga aaacacgaca tgtgaatatc ctactcttca
1621tgggctattc cacaaagcca caactggcta ttgttaccca gtggtgtgag ggctccagct
1681tgtatcacca tctccatatc attgagacca aatttgagat gatcaaactt atagatattg
1741cacgacagac tgcacagggc atggattact tacacgccaa gtcaatcatc cacagagacc
1801tcaagagtaa taatatattt cttcatgaag acctcacagt aaaaataggt gattttggtc
1861tagctacagt gaaatctcga tggagtgggt cccatcagtt tgaacagttg tctggatcca
1921ttttgtggat ggcaccagaa gtcatcagaa tgcaagataa aaatccatac agctttcagt
1981cagatgtata tgcatttgga attgttctgt atgaattGAT GACTGGACAG TTACCTTATT
2041CAAACATCAA CAACAGGGAC CAGATAATTT TTATGGTGGG ACGAGGATAC CTGTCTCCAG
2101ATCTCAGTAA GGTACGGAGT AACTGTCCAA AAGCCATGAA GAGATTAATG GCAGAGTGCC
2161TCAAAAAGAA AAGAGATGAG AGACCACTCT TTCCCCAAAT TCTCGCCTCT ATTGAGCTGC
2221TGGCCCGCTC ATTGCCAAAA ATTCACCGCA GTGCATCAGA ACCCTCCTTG AATCGGGCTG
2281GTTTCCAAAC AGAGGATTTT AGTCTATATG CTTGTGCTTC TCCAAAAACA CCCATCCAGG
2341CAGGGGGATA TGGTGCGTTT CCTGTCCACT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG
2401ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2461GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG
2521TTCGGAATTC TGTGTTGTAC CCACGCGTTA AGTCgacaat caacctctgg attacaaaat
2581ttgtgaaaga tt

Download FASTA (ORF) (Full)