Construct: ORF TRCN0000489580
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019261.1_s317c1
- DNA Barcode:
- TGTCACCAGTAGTAGCACCTGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AVPR2 (554)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489580
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 554 | AVPR2 | arginine vasopressin recept... | NM_000054.6 | 99.8% | 99.7% | 927A>G;1113_1114insG |
| 2 | human | 554 | AVPR2 | arginine vasopressin recept... | NM_001146151.3 | 82.6% | 82.5% | (many diffs) |
| 3 | human | 554 | AVPR2 | arginine vasopressin recept... | NR_027419.2 | 51.4% | (many diffs) | |
| 4 | mouse | 12000 | Avpr2 | arginine vasopressin recept... | NM_019404.2 | 83.9% | 87.3% | (many diffs) |
| 5 | mouse | 12000 | Avpr2 | arginine vasopressin recept... | XM_006527756.3 | 83.9% | 87.3% | (many diffs) |
| 6 | mouse | 12000 | Avpr2 | arginine vasopressin recept... | XM_017318365.1 | 83.8% | 87% | (many diffs) |
| 7 | mouse | 12000 | Avpr2 | arginine vasopressin recept... | NM_001276299.1 | 35.6% | 37.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1191
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgctc atggcgtcca ccacttccgc tgtgcctggg catccctctc 121 tgcccagcct gcccagcaac agcagccagg agaggccact ggacacccgg gacccgctgc 181 tagcccgggc ggagctggcg ctgctctcca tagtctttgt ggctgtggcc ctgagcaatg 241 gcctggtgct ggcggcccta gctcggcggg gccggcgggg ccactgggca cccatacacg 301 tcttcattgg ccacttgtgc ctggccgacc tggccgtggc tctgttccaa gtgctgcccc 361 agctggcctg gaaggccacc gaccgcttcc gtgggccaga tgccctgtgt cgggccgtga 421 agtatctgca gatggtgggc atgtatgcct cctcctacat gatcctggcc atgacgctgg 481 accgccaccg tgccatctgc cgtcccatgc tggcgtaccg ccatggaagt ggggctcact 541 ggaaccggcc ggtgctagtg gcttgggcct tctcgctcct tctcagcctg ccccagctct 601 tcatcttcgc ccagcgcaac gtggaaggtg gcagcggggt cactgactgc tgggcctgct 661 ttgcggagcc ctggggccgt cgcacctatg tcacctggat tgccctgatg gtgttcgtgg 721 cacctaccct gggtatcgcc gcctgccagg tgctcatctt ccgggagatt catgccagtc 781 tggtgccagg gccatcagag aggccTGGGG GGCGCCGCAG GGGACGCCGG ACAGGCAGCC 841 CCGGTGAGGG AGCCCACGTG TCAGCAGCTG TGGCCAAGAC TGTGAGGATG ACGCTAGTGA 901 TTGTGGTCGT CTATGTGCTG TGCTGGGCAC CCTTCTTCCT GGTGCAGCTG TGGGCCGCGT 961 GGGACCCGGA GGCACCTCTG GAAGGGGCGC CCTTTGTGCT GCTCATGTTG CTGGCCAGCC 1021 TCAACAGCTG CACCAACCCC TGGATCTATG CATCTTTCAG CAGCAGCGTG TCCTCAGAGC 1081 TGCGAAGCTT GCTCTGCTGT GCCCGGGGAC GCACCCCACC CAGCCTGGGT CCCCAAGATG 1141 AGTCCTGCAC CACCGCCAGC TCCTCCCTGG CCAAGGACAC TTCATCGGAC CCAGCTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGATGTCACC AGTAGTAGCA CCTGGCTACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt