Construct: ORF TRCN0000489581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019258.1_s317c1
- DNA Barcode:
- GGCTGGTCTGCCGTAAGAGATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LHCGR (3973)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3973 | LHCGR | luteinizing hormone/choriog... | NM_000233.4 | 100% | 100% | |
| 2 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_017004089.1 | 80.7% | 78.6% | (many diffs) |
| 3 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_011532831.1 | 69.6% | 69.6% | 0_1ins636 |
| 4 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_017004090.1 | 69.6% | 69.6% | 0_1ins636 |
| 5 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_006712015.3 | 55.4% | 54.7% | (many diffs) |
| 6 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_005264309.3 | 54.3% | 54.3% | 0_1ins957 |
| 7 | human | 3973 | LHCGR | luteinizing hormone/choriog... | XM_011532834.2 | 54.3% | 54.3% | 0_1ins957 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2172
- ORF length:
- 2097
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaag cagcggttct cggcgctgca gctgctgaag ctgctgctgc 121 tgctgcagcc gccgctgcca cgagcgctgc gcgaggcgct ctgccctgag ccctgcaact 181 gcgtgcccga cggcgccctg cgctgccccg gccccacggc cggtctcact cgactatcac 241 ttgcctacct ccctgtcaaa gtgatcccat ctcaagcttt cagaggactt aatgaggtca 301 taaaaattga aatctctcag attgattccc tggaaaggat agaagctaat gcctttgaca 361 acctcctcaa tttgtctgaa atactgatcc agaacaccaa aaatctgaga tacattgagc 421 ccggagcatt tataaatctt ccccgattaa aatacttgag catctgtaac acaggcatca 481 gaaagtttcc agatgttacg aaggtcttct cctctgaatc aaatttcatt ctggaaattt 541 gtgataactt acacataacc accataccag gaaatgcttt tcaagggatg aataatgaat 601 ctgtaacact caaactatat ggaaatggat ttgaagaagt acaaagtcat gcattcaatg 661 ggacgacact gacttcactg gagctaaagg aaaacgtaca tctggagaag atgcacaatg 721 gagccttccg tggggccaca gggccgaaaa ccttggatat ttcttccacc aaattgcagg 781 ccctgccgag ctatggccta gagtccattc agaggctaat tgccacgtca tcctattctc 841 taaaaaaatt gccatcaaga gaaacatttg tcaatctcct ggaggccacg ttgacttacc 901 ccagccactg ctgtgctttt agaaacttgc caacaaaaga acagaatttt tcacattcca 961 tttctgaaaa cttttccaaa caatgtgaaa gcacagtaag gaaagtgaat aacaaaacac 1021 tttattcttc catgcttgct gagagtgaac tgagtggctg ggactatgaa tatggtttct 1081 gcttacccaa gacaccccga tgtgctcctg aaccagatgc ttttaatccc tgtgaagata 1141 ttatgggcta tgacttcctt agggtcctga tttggctgat taatattcta gccatcatgg 1201 gaaacatgac tgttcttttt gttctcctga caagtcgtta caaacttaca gtgcctcgtt 1261 ttctcatgtg caatctctcc tttgcagact tttgcatggg gctctatctg ctgctcatag 1321 cctcagttga ttcccaaacc aagggccagt actataacca tgccatagac tggcagacag 1381 ggagtgggtg cagcactgct ggctttttca ctgtattcgc aagtgaactt tctgtctaca 1441 ccctcaccgt catcactcta gaaagatggc acaccatcac ctatgctatt cacctggacc 1501 aaaagctgcg attaagacat gccattctga ttatgcttgg aggatggctc ttttcttctc 1561 taattgctat gttgcccctt gtcggtgtca gcaattacat gaaggtcagt atttgcttcc 1621 ccatggatgt ggaaaccact ctctcacaag tctatatatt aaccatcctg attctcaatg 1681 tggtggcctt cttcataatt tgtgcttgct acattaaaat ttattttgca gttcgaaacc 1741 cagaattaat ggctaccaat aaagatacaa agattgctaa gaaaatggca atcctcatct 1801 tcaccgattt cacctgcatg gCACCTATCT CTTTTTTTGC CATCTCAGCT GCCTTCAAAG 1861 TACCTCTTAT CACAGTAACC AACTCTAAAG TTTTACTGGT TCTTTTTTAT CCCATCAATT 1921 CTTGTGCCAA TCCATTTCTG TATGCAATAT TCACTAAGAC ATTCCAAAGA GATTTCTTTC 1981 TTTTGCTGAG CAAATTTGGC TGCTGTAAAC GTCGGGCTGA ACTTTATAGA AGGAAAGATT 2041 TTTCAGCTTA CACCTCCAAC TGCAAAAATG GCTTCACTGG ATCAAATAAG CCTTCTCAAT 2101 CCACCTTGAA GTTGTCCACA TTGCACTGTC AAGGTACAGC TCTCCTAGAC AAGACTCGCT 2161 ACACAGAGTG TGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2221 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2281 CGATTTCTTG GCTTTATATA ATCTTGTGGA AAGGACGAGG CTGGTCTGCC GTAAGAGATT 2341 TAACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt