Construct: ORF TRCN0000489611
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020848.1_s317c1
- DNA Barcode:
- TGCCGATGATATCCATACTTCAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HTR1F (3355)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489611
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | NM_000866.4 | 100% | 100% | |
| 2 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | NM_001322208.2 | 100% | 100% | |
| 3 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | NM_001322209.2 | 100% | 100% | |
| 4 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | NM_001322210.2 | 100% | 100% | |
| 5 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | XM_005264751.3 | 100% | 100% | |
| 6 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | XM_011533664.2 | 100% | 100% | |
| 7 | mouse | 15557 | Htr1f | 5-hydroxytryptamine (seroto... | NM_008310.3 | 87.9% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1170
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatttc ttaaattcat ctgatcaaaa cttgacctca gaggaactgt 121 taaacagaat gccatccaaa attctggtgt ccctcactct gtctgggctg gcactgatga 181 caacaactat caactccctt gtgatcgctg caattattgt gacccggaag ctgcaccatc 241 cagccaatta tttaatttgt tcccttgcag tcacagattt tcttgtggct gtcctggtga 301 tgcccttcag cattgtgtat attgtgagag agagctggat tatggggcaa gtggtctgtg 361 acatttggct gagtgttgac attacctgct gcacgtgctc catcttgcat ctctcagcta 421 tagctttgga tcggtatcga gcaatcacag atgctgttga gtatgccagg aaaaggactc 481 caaagcatgc tggcattatg attacaatag tttggattat atctgttttt atctctatgc 541 ctcctctatt ctggaggcac caaggaacta gcagagatga tgaatgcatc atcaagcacg 601 accacattgt ttccaccatt tactcaacat ttggagcttt ctacatccca ctggcattga 661 ttttgatcct ttactacaaa atatatagag cagcaaagac attataccac aagagacaag 721 caagtaggat tgcaaaggag gaggtgaatg gccaagtcct tttggagagt ggtgagaaaa 781 gcactaaatc agtttccaca tcctatgtac tagaaaagtc tttatctgac ccatcaacag 841 actttgataa aattcatagc acagtgagaa gtctcaggtc tgaattcaag catgagaaat 901 cttGGAGAAG GCAAAAGATC TCAGGTACAA GAGAACGGAA AGCAGCCACT ACCCTGGGAT 961 TAATCTTGGG TGCATTTGTA ATATGTTGGC TTCCTTTTTT TGTAAAAGAA TTAGTTGTTA 1021 ATGTCTGTGA CAAATGTAAA ATTTCTGAAG AAATGTCCAA TTTTTTGGCA TGGCTTGGGT 1081 ATCTCAATTC CCTTATAAAT CCACTGATTT ACACAATCTT TAATGAAGAC TTCAAGAAAG 1141 CATTCCAAAA GCTTGTGCGA TGTCGATGTT AGGACCCAGC TTTCTTGTAC AAAGTGGTTG 1201 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1261 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 1321 CCGATGATAT CCATACTTCA GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1381 ttgtgaaaga tt