Construct: ORF TRCN0000489611

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020848.1_s317c1
DNA Barcode:
TGCCGATGATATCCATACTTCAGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
HTR1F (3355)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489611
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3355 HTR1F 5-hydroxytryptamine recepto... NM_000866.4 100% 100%
2 human 3355 HTR1F 5-hydroxytryptamine recepto... NM_001322208.2 100% 100%
3 human 3355 HTR1F 5-hydroxytryptamine recepto... NM_001322209.2 100% 100%
4 human 3355 HTR1F 5-hydroxytryptamine recepto... NM_001322210.2 100% 100%
5 human 3355 HTR1F 5-hydroxytryptamine recepto... XM_005264751.3 100% 100%
6 human 3355 HTR1F 5-hydroxytryptamine recepto... XM_011533664.2 100% 100%
7 mouse 15557 Htr1f 5-hydroxytryptamine (seroto... NM_008310.3 87.9% 94.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1170
ORF length:
1098
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggatttc ttaaattcat ctgatcaaaa cttgacctca gaggaactgt
121taaacagaat gccatccaaa attctggtgt ccctcactct gtctgggctg gcactgatga
181caacaactat caactccctt gtgatcgctg caattattgt gacccggaag ctgcaccatc
241cagccaatta tttaatttgt tcccttgcag tcacagattt tcttgtggct gtcctggtga
301tgcccttcag cattgtgtat attgtgagag agagctggat tatggggcaa gtggtctgtg
361acatttggct gagtgttgac attacctgct gcacgtgctc catcttgcat ctctcagcta
421tagctttgga tcggtatcga gcaatcacag atgctgttga gtatgccagg aaaaggactc
481caaagcatgc tggcattatg attacaatag tttggattat atctgttttt atctctatgc
541ctcctctatt ctggaggcac caaggaacta gcagagatga tgaatgcatc atcaagcacg
601accacattgt ttccaccatt tactcaacat ttggagcttt ctacatccca ctggcattga
661ttttgatcct ttactacaaa atatatagag cagcaaagac attataccac aagagacaag
721caagtaggat tgcaaaggag gaggtgaatg gccaagtcct tttggagagt ggtgagaaaa
781gcactaaatc agtttccaca tcctatgtac tagaaaagtc tttatctgac ccatcaacag
841actttgataa aattcatagc acagtgagaa gtctcaggtc tgaattcaag catgagaaat
901cttGGAGAAG GCAAAAGATC TCAGGTACAA GAGAACGGAA AGCAGCCACT ACCCTGGGAT
961TAATCTTGGG TGCATTTGTA ATATGTTGGC TTCCTTTTTT TGTAAAAGAA TTAGTTGTTA
1021ATGTCTGTGA CAAATGTAAA ATTTCTGAAG AAATGTCCAA TTTTTTGGCA TGGCTTGGGT
1081ATCTCAATTC CCTTATAAAT CCACTGATTT ACACAATCTT TAATGAAGAC TTCAAGAAAG
1141CATTCCAAAA GCTTGTGCGA TGTCGATGTT AGGACCCAGC TTTCTTGTAC AAAGTGGTTG
1201ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1261GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG
1321CCGATGATAT CCATACTTCA GCACGCGTTA AGTCgacaat caacctctgg attacaaaat
1381ttgtgaaaga tt

Download FASTA (ORF) (Full)