Construct: ORF TRCN0000489613
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020486.1_s317c1
- DNA Barcode:
- CACCTTAGGGTACTTGCCGCTGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- IRAK1 (3654)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489613
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001569.4 | 97.7% | 97.4% | (many diffs) |
| 2 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001025242.2 | 93.5% | 93.3% | (many diffs) |
| 3 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001025243.2 | 90.6% | 90.5% | (many diffs) |
| 4 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | XM_005274668.4 | 90.2% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2163
- ORF length:
- 2091
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccggg gggccgggcc cgggggagcc cgcagccccc ggcgcccagc 121 acttcttgta cgaggtgccg ccctgggtca tgtgccgctt ctacaaagtg atggacgccc 181 tggagcccgc cgactggtgc cagttcgccg ccctgatcgt gcgcgaccag accgagctgc 241 ggctgtgcga gcgctccggg cagcgcacgg ccagcgtcct gtggccctgg atcaaccgca 301 acgcccgtgt ggccgacctc gtgcacatcc tcacgcacct gcagctgctc cgtgcgcggg 361 acatcatcac agcctggcac cctcccgccc cgcttccgtc cccaggcacc actgccccga 421 ggcccagcag catccctgca cccgccgagg ccgaggcctg gagcccccgg aagttgccat 481 cctcagcctc caccttcctc tccccagctt ttccaggctc ccagacccat tcagggcctg 541 agctcggcct ggttccaagc cctgcttccc tgtggcctcc accgccatct ccagcccctt 601 cttctaccaa gccaggccca gagagctcag tgtccctcct gcagggagcc cgcccctctc 661 cgttttgctg gcccctctgt gagatttccc ggggcaccca caacttctcg gaggagctca 721 agatcgggga gggtggcttt gggtgcgtgt accgggcggt gatgaggaac acggtgtatg 781 ctgtgaagag gctgaaggag aacgctgacc tggagtggac tgcagtgaag cagagcttcc 841 tgaccgaggt ggagcagctg tccaggtttc gtcacccaaa cattgtggac tttgctggct 901 actgtgctca gaacggcttc tactgcctgg tgtacggctt cctgcccaac ggctccctgg 961 aggaccgtct ccactgccag acccaggcct gcccacctct ctcctggcct cagcgactgg 1021 acatccttct gggtacagcc cgggcaattc agtttctaca tcaggacagc cccagcctca 1081 tccatggaga catcaagagt tccaacgtcc ttctggatga gaggctgaca cccaagctgg 1141 gagactttgg cctggcccgg ttcagccgct ttgccgggtc cagccccagc cagagcagca 1201 tggtggcccg gacacagaca gtgcggggca ccctggccta cctgcccgag gagtacatca 1261 agacgggaag gctggctgtg gacacggaca ccttcagctt tggggtggta gtgctagaga 1321 ccttggctgg tcagagggct gtgaagacgc acggtgccag gaccaagtat ctgaaagacc 1381 tggtggaaga ggaggctgag gaggctggag tggctttgag aagcacccag agcacactgc 1441 aagcaggtct ggctgcagat gcctgggctg ctcccatcgc catgcagatc tacaagaagc 1501 gcctgggcca gctggcctgc tgctgcctgc accgccgggc caaaaggagg cctcctatga 1561 cccaggtgta cgagaggcta gagaagctgc aggcagtggt ggcgggggtg cccgggcatt 1621 tggaggccgc cagctgcatc cccccttccc cgcaggagaa ctcctacgtg tccagcactg 1681 gcagagccca cagtggggct gctccatggc agcccctggc agcgccatca ggagccagtg 1741 cccaggcagc agagcagctg cagagaggcc ccaaccagcc cgtggagagt gacgagagcc 1801 taggcggcct ctctgctgcc ctgcgctcct ggcacttgac tccaagctgc cctctggacc 1861 cagcaccccT CAGGGAGGCC GGCTGTCCTC AGGGGGACAC GGCAGGAGAA TCGAGCTGGG 1921 GGAGTGGCCC AGGATCCCGG CCCACAGCCG TGGAAGGACT GGCCCTTGGC AGCTCTGCAT 1981 CATCATCGTC AGAGCCACCG CAGATTATCA TCAACCCTGC CCGACAGAAG ATGGTCCAGA 2041 AGCTGGCCCT GTACGAGGAT GGGGCCCTGG ACAGCCTGCA GCTGCTGTCG TCCAGCTCCC 2101 TCCCAGGCTT GGGCCTGGAA CAGGACAGGC AGGGGCCCGA AGAAAGTGAT GAATTTCAGA 2161 GCTAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2221 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2281 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACACCTTAGG GTACTTGCCG CTGTAACGCG 2341 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt