Construct: ORF TRCN0000489614
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020339.1_s317c1
- DNA Barcode:
- GCGCCCCAGGGCCCTTGACTTACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CAMK1G (57172)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489614
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57172 | CAMK1G | calcium/calmodulin dependen... | NM_020439.3 | 99.9% | 99.7% | 807G>C |
| 2 | human | 57172 | CAMK1G | calcium/calmodulin dependen... | XM_017001866.2 | 99.9% | 99.7% | 807G>C |
| 3 | human | 57172 | CAMK1G | calcium/calmodulin dependen... | XM_017001867.1 | 66.3% | 66.1% | 0_1ins480;327G>C |
| 4 | mouse | 215303 | Camk1g | calcium/calmodulin-dependen... | NM_144817.2 | 87.6% | 91.8% | (many diffs) |
| 5 | mouse | 215303 | Camk1g | calcium/calmodulin-dependen... | XM_011238932.2 | 85.2% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1500
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggtcga aaggaagaag atgactgcag ttcctggaag aaacagacca 121 ccaacatccg gaaaaccttc atttttatgg aagtgctggg atcaggagct ttctcagaag 181 ttttcctggt gaagcaaaga ctgactggga agctctttgc tctgaagtgc atcaagaagt 241 cacctgcctt ccgggacagc agcctggaga atgagattgc tgtgttgaaa aagatcaagc 301 atgaaaacat tgtgaccctg gaggacatct atgagagcac cacccactac tacctggtca 361 tgcagcttgt ttctggtggg gagctctttg accggatcct ggagcggggt gtctacacag 421 agaaggatgc cagtctggtg atccagcagg tcttgtcggc agtgaaatac ctacatgaga 481 atggcatcgt ccacagagac ttaaagcccg aaaacctgct ttaccttacc cctgaagaga 541 actctaagat catgatcact gactttggtc tgtccaagat ggaacagaat ggcatcatgt 601 ccactgcctg tgggacccca ggctacgtgg ctccagaagt gctggcccag aaaccctaca 661 gcaaggctgt ggattgctgg tccatcggcg tcatcaccta catattgctc tgtggatacc 721 ccccattcta tgaagaaacg gagtctaagc ttttcgagaa gatcaaggag ggctactatg 781 agtttgagtc tccattctgg gatgacattt ctgagtcagc caaggacttt atttgccact 841 tgcttgagaa ggatccgaac gagcggtaca cctgtgacaa ggccttgagt catccctgga 901 ttgacggaaa cacagccctc caccgggaca tctacccatc agtcagcctc cagatccaga 961 agaactttgc taagagcaag tggaggcaag ccttcaacgc agcagctgtg gtgcaccaca 1021 tgaggaagct acacatgaac ctgcacagcc cgggcgtccg cccagaggtg gagaacaggc 1081 cgcctgaaac tcaagcctca gaaacctcta gacccagctc ccctgagatc accatcaccg 1141 aggcacctgt ccTGGACCAC AGTGTAGCAC TCCCTGCCCT GACCCAATTA CCCTGCCAGC 1201 ATGGCCGCCG GCCCACTGCC CCTGGTGGCA GGTCCCTCAA CTGCCTGGTC AATGGCTCCC 1261 TCCACATCAG CAGCAGCCTG GTGCCCATGC ATCAGGGGTC CCTGGCCGCC GGGCCCTGTG 1321 GCTGCTGCTC CAGCTGCCTG AACATTGGGA GCAAAGGAAA GTCCTCCTAC TGCTCTGAGC 1381 CCACACTCCT CAAAAAGGCC AACAAAAAAC AGAACTTCAA GTCGGAGGTC ATGGTACCAG 1441 TTAAAGCCAG TGGCAGCTCC CACTGCCGGG CAGGGCAGAC TGGAGTCTGT CTCATTATGT 1501 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1561 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1621 GGCTTTATAT ATCTTGTGGA AAGGACGAGC GCCCCAGGGC CCTTGACTTA CGACGCGTTA 1681 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt