Construct: ORF TRCN0000489621
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020036.1_s317c1
- DNA Barcode:
- TGTCGATCGCGCACCGGAACAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRM4 (2914)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489621
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_000841.4 | 99.8% | 99.8% | 660T>C;1458T>C;2736_2737insG |
| 2 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001256811.2 | 94.7% | 94.6% | (many diffs) |
| 3 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001256813.3 | 84.2% | 81% | (many diffs) |
| 4 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001256809.2 | 82.8% | 78.3% | (many diffs) |
| 5 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001256812.2 | 81.1% | 81% | (many diffs) |
| 6 | human | 2914 | GRM4 | glutamate metabotropic rece... | NM_001282847.2 | 66.1% | 66.1% | 0_1ins924;534T>C;1812_1813insG |
| 7 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | NM_001291045.1 | 89.9% | 97% | (many diffs) |
| 8 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_006524317.3 | 89.9% | 97% | (many diffs) |
| 9 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_011246475.2 | 89.9% | 97% | (many diffs) |
| 10 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | NM_001013385.2 | 81.1% | 86.6% | (many diffs) |
| 11 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_006524319.3 | 69.7% | 75% | (many diffs) |
| 12 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_006524320.3 | 60.1% | 64.6% | (many diffs) |
| 13 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_017317482.1 | 60.1% | 64.6% | (many diffs) |
| 14 | mouse | 268934 | Grm4 | glutamate receptor, metabot... | XM_006524321.3 | 54% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2808
- ORF length:
- 2739
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcctgggaag agaggcttgg gctggtggtg ggcccggctg cccctttgcc 121 tgctcctcag cctttacggc ccctggatgc cttcctccct gggaaagccc aaaggccacc 181 ctcacatgaa ttccatccgc atagatgggg acatcacact gggaggcctg ttcccggtgc 241 atggccgggg ctcagagggc aagccctgtg gagaacttaa gaaggaaaag ggcatccacc 301 ggctggaggc catgctgttc gccctggatc gcatcaacaa cgacccggac ctgctgccta 361 acatcacgct gggcgcccgc attctggaca cctgctccag ggacacccat gccctcgagc 421 agtcgctgac ctttgtgcag gcgctcatcg agaaggatgg cacagaggtc cgctgtggca 481 gtggcggccc acccatcatc accaagcctg aacgtgtggt gggtgtcatc ggtgcttcag 541 ggagctcggt ctccatcatg gtggccaaca tccttcgcct cttcaagata ccccagatca 601 gctacgcctc cacagcgcca gacctgagtg acaacagccg ctacgacttc ttctcccgcg 661 tggtgccctc ggacacgtac caggcccagg ccatggtgga catcgtccgt gccctcaagt 721 ggaactacgt gtccacagtg gcctcggagg gcagctatgg tgagagcggt gtggaggcct 781 tcatccagaa gtcccgtgag gacgggggcg tgtgcatcgc ccagtcggtg aagataccac 841 gggagcccaa ggcaggcgag ttcgacaaga tcatccgccg cctcctggag acttcgaacg 901 ccagggcagt catcatcttt gccaacgagg atgacatcag gcgtgtgctg gaggcagcac 961 gaagggccaa ccagacaggc catttcttct ggatgggctc tgacagctgg ggctccaaga 1021 ttgcacctgt gctgcacctg gaggaggtgg ctgagggtgc tgtcacgatc ctccccaaga 1081 ggatgtccgt acgaggcttc gaccgctact tctccagccg cacgctggac aacaaccggc 1141 gcaacatctg gtttgccgag ttctgggagg acaacttcca ctgcaagctg agccgccacg 1201 ccctcaagaa gggcagccac gtcaagaagt gcaccaaccg tgagcgaatt gggcaggatt 1261 cagcttatga gcaggagggg aaggtgcagt ttgtgatcga tgccgtgtac gccatgggcc 1321 acgcgctgca cgccatgcac cgtgacctgt gtcccggccg cgtggggctc tgcccgcgca 1381 tggaccctgt agatggcacc cagctgctta agtacatccg aaacgtcaac ttctcaggca 1441 tcgcagggaa ccctgtgacc ttcaatgaga atggagatgc gcctgggcgc tatgacatct 1501 accaatacca gctgcgcaac gattccgccg agtacaaggt cattggctcc tggactgacc 1561 acctgcacct tagaatagag cggatgcact ggccggggag cgggcagcag ctgccccgct 1621 ccatctgcag cctgccctgc caaccgggtg agcggaagaa gacagtgaag ggcatgcctt 1681 gctgctggca ctgcgagcct tgcacagggt accagtacca ggtggaccgc tacacctgta 1741 agacgtgtcc ctatgacatg cggcccacag agaaccgcac gggctgccgg cccatcccca 1801 tcatcaagct tgagtggggc tcgccctggg ccgtgctgcc cctcttcctg gccgtggtgg 1861 gcatcgctgc cacgttgttc gtggtgatca cctttgtgcg ctacaacgac acgcccatcg 1921 tcaaggcctc gggccgtgaa ctgagctacg tgctgctggc aggcatcttc ctgtgctatg 1981 ccaccacctt cctcatgatc gctgagcccg accttggcac ctgctcgctg cgccgaatct 2041 tcctgggact agggatgagc atcagctatg cagccctgct caccaagacc aaccgcatct 2101 accgcatctt cgagcagggc aagcgctcgg tcagtgcccc acgcttcatc agccccgcct 2161 cacagctggc catcaccttc agcctcatct cgctgcagct gctgggcatc tgtgtgtggt 2221 ttgtggtgga cccctcccac tcggtggtgg acttccagga ccagcggaca ctcgaccccc 2281 gcttcgccag gggtgtgctc aagtgtgaca tctcggacct gtcgctcatc tgcctgctgg 2341 gctacagcat gctgctcatg gtcacgtgca ccgtgtatgc catcaagaca cgcggcgtgc 2401 ccgagacctt caatgaggcc aagcccattg gcttcaccat gtacaccact tgcatcgtct 2461 ggctggccTT CATCCCCATC TTCTTTGGCA CCTCGCAGTC GGCCGACAAG CTGTACATCC 2521 AGACGACGAC GCTGACGGTC TCGGTGAGTC TGAGCGCCTC GGTGTCCCTG GGAATGCTCT 2581 ACATGCCCAA AGTCTACATC ATCCTCTTCC ACCCGGAGCA GAACGTGCCC AAGCGCAAGC 2641 GCAGCCTCAA AGCCGTCGTT ACGGCGGCCA CCATGTCCAA CAAGTTCACG CAGAAGGGCA 2701 ACTTCCGGCC CAACGGAGAG GCCAAGTCTG AGCTCTGCGA GAACCTTGAG GCCCCAGCGC 2761 TGGCCACCAA ACAGACTTAC GTCACTTACA CCAACCATGC AATCGACCCA GCTTTCTTGT 2821 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2881 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2941 GAAAGGACGA TGTCGATCGC GCACCGGAAC AAAAACGCGT TAAGTCgaca atcaacctct 3001 ggattacaaa atttgtgaaa gatt