Construct: ORF TRCN0000489624
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019703.1_s317c1
- DNA Barcode:
- GCCTTACCCTCATTAGGAGTCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADCY4 (196883)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489624
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 196883 | ADCY4 | adenylate cyclase 4 | NM_001198568.2 | 99.9% | 99.9% | 3231_3232insG |
| 2 | human | 196883 | ADCY4 | adenylate cyclase 4 | NM_001198592.2 | 99.9% | 99.9% | 3231_3232insG |
| 3 | human | 196883 | ADCY4 | adenylate cyclase 4 | NM_139247.4 | 99.9% | 99.9% | 3231_3232insG |
| 4 | mouse | 104110 | Adcy4 | adenylate cyclase 4 | NM_080435.1 | 85.9% | 91.7% | (many diffs) |
| 5 | mouse | 104110 | Adcy4 | adenylate cyclase 4 | XM_006518323.1 | 85.9% | 91.7% | (many diffs) |
| 6 | mouse | 104110 | Adcy4 | adenylate cyclase 4 | XM_006518324.1 | 85.9% | 91.7% | (many diffs) |
| 7 | mouse | 104110 | Adcy4 | adenylate cyclase 4 | XM_006518325.1 | 75.9% | 80.6% | (many diffs) |
| 8 | mouse | 104110 | Adcy4 | adenylate cyclase 4 | XM_006518326.2 | 60.9% | 64.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3309
- ORF length:
- 3234
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc cgcctcttca gcccccggcc gccccccagc gaagacctct 121 tctacgagac ctactacagc ctgagccagc agtacccgct gctgctgctg ctgctgggga 181 tcgtgctctg tgcgctcgcg gcgctgctcg cagtggcctg ggccagcggc agggagctga 241 cctcagaccc gagcttcctg accactgtgc tgtgcgcgct gggcggcttc tcgctgctgc 301 tgggcctcgc ttcccgggag cagcgactgc agcgctggac gcgtcccctg tccggcttgg 361 tatgggtcgc gctgctagcg ctaggccacg ccttcctgtt caccgggggc gtggtgagcg 421 cctgggacca ggtgtcctat tttctcttcg tcatcttcac ggcgtatgcc atgctgccct 481 tgggcatgcg ggacgccgcc gtcgcgggcc tcgcctcctc actctcgcat ctgctggtcc 541 tcgggctgta tcttgggcca cagccggact cacggcctgc actgctgccg cagttggcag 601 caaacgcagt gctgttcctg tgcgggaacg tggcaggagt gtaccacaag gcgctgatgg 661 agcgcgccct gcgggccacg ttccgggagg cactcagctc cctgcactca cgccggcggc 721 tggacaccga gaagaagcac caggaacacc ttctcttgtc catccttcct gcctacctgg 781 cccgagagat gaaggcagag atcatggcac ggctgcaggc aggacagggg tcacggccag 841 agagcactaa caatttccac agcctctatg tcaagaggca ccagggagtc agcgtgctgt 901 atgctgacat cgtgggcttc acgcggctgg ccagcgagtg ttcccctaag gagctggtgc 961 tcatgctcaa tgagctcttt ggcaagttcg accagattgc caaggagcat gaatgcatgc 1021 ggatcaagat cctgggggac tgttactact gtgtctctgg gctgccactc tcactgccag 1081 accatgccat caactgcgtg cgcatgggcc tggacatgtg ccgggccatc aggaaactgc 1141 gggcagccac tggcgtggac atcaacatgc gtgtgggcgt gcactcaggc agcgtactgt 1201 gtggagtcat cgggctgcag aagtggcagt acgacgtttg gtcacatgat gtcacactgg 1261 ctaaccacat ggaggcaggc ggtgtaccag ggcgagtgca catcacaggg gctaccctgg 1321 ccctgctggc aggggcttat gctgtggagg acgcaggcat ggagcatcgg gacccctacc 1381 ttcgggagct aggggagcct acctatctgg tcatcgatcc acgggcagag gaggaggatg 1441 agaagggcac tgcaggaggc ttgctgtcct cgcttgaggg cctcaagatg cgtccatcac 1501 tgctgatgac ccgttacctg gagtcctggg gcgcagccaa gccttttgcc cacctgagcc 1561 acggagacag ccctgtgtcc acctccaccc ctctcccgga gaagaccctg gcttccttca 1621 gcacccagtg gagcctggat cggagccgta ccccccgggg actagatgat gaactggaca 1681 ccggggatgc caagttcttc caggtcattg agcagctcaa ctcgcagaaa cagtggaagc 1741 agtcgaagga cttcaaccca ctgacactgt acttcagaga gaaggagatg gagaaagagt 1801 accgactctc tgcaatcccc gccttcaaat actatgaagc ctgcaccttc ctggtttttc 1861 tctccaactt catcatccag atgctagtga caaacaggcc cccagctctg gccatcacgt 1921 atagcatcac cttcctcctc ttcctcctca tcctttttgt ctgcttctca gaggacctga 1981 tgaggtgtgt cctgaaaggc cccaagatgc tgcactggct gcctgcactg tctggcctgg 2041 tggccacacg accaggactg agaatagcct tgggcaccgc caccatcctc cttgtctttg 2101 ccatggccat taccagcctg ttcttcttcc caacatcatc agactgccct ttccaagctc 2161 ccaatgtgtc ctccatgatt tccaacctct cctgggagct ccctgggtct ctgcctctca 2221 tcagtgtccc atactccatg cactgctgca cgctgggctt cctctcctgc tccctctttc 2281 tgcacatgag cttcgagctg aagctgctgc tgctcctgct gtggctggcg gcatcctgct 2341 ccctcttcct gcactcccat gcctggctgt cggaatgcct catcgtccgc ctctatctgg 2401 gccccttgga ctccaggccc ggagtgctga aggagcccaa actgatgggt gctatctcct 2461 tcttcatctt cttcttcacc ctccttgtcc tggctcgcca gaatgagtac tactgccgcc 2521 tggacttcct gtggaagaag aagctgaggc aggagaggga ggagacagag acgatggaga 2581 acctgactcg gctgctcttg gagaacgtgc tccctgcaca cgtggccccc cagttcattg 2641 gccagaaccg gcgcaacgag gatctctacc accagtccta tgaatgcgtt tgtgtcctct 2701 tcgcctcagt cccagacttc aaggagttct actctgaatc caacatcaat catgagggcc 2761 tagagtgtct gaggctgctc aatgagataa ttgctgattt tgatgagctg ctctccaagc 2821 ccaagttcag tggggtggag aagatcaaga ccatcggcag cacctacatg gcagccacag 2881 gcttaaatgc cacctctgga caggatgcac aacaggatgc tgaacggagc tgcagccacc 2941 ttggcactat ggtggaattt gccgtggccc tggggtctaa gctggacgtc atcaacaagc 3001 attcattcaa caacttccgc cTGCGAGTGG GGTTGAACCA TGGACCCGTA GTAGCTGGAG 3061 TTATTGGGGC CCAGAAGCCG CAATATGACA TTTGGGGCAA CACAGTGAAC GTGGCCAGCC 3121 GCATGGAGAG TACAGGAGTC CTTGGCAAAA TCCAAGTGAC TGAGGAGACA GCATGGGCCC 3181 TACAGTCCCT GGGCTACACC TGCTACAGCC GGGGTGTCAT CAAGGTGAAA GGCAAAGGGC 3241 AGCTCTGCAC CTACTTCCTG AACACAGACT TGACACGAAC TGGACCTCCT TCAGCTACCC 3301 TAGGCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 3361 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3421 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCTTACCC TCATTAGGAG TCTGTACGCG 3481 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt