Construct: ORF TRCN0000489632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021895.1_s317c1
- DNA Barcode:
- TATCATGCTGACCCAAGCCCCCGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RASGRP1 (10125)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10125 | RASGRP1 | RAS guanyl releasing protein 1 | NM_005739.4 | 100% | 100% | |
| 2 | human | 10125 | RASGRP1 | RAS guanyl releasing protein 1 | NM_001128602.2 | 95.6% | 95.6% | 1323_1324ins105 |
| 3 | human | 10125 | RASGRP1 | RAS guanyl releasing protein 1 | XM_017021860.1 | 93.9% | 93.9% | 0_1ins144 |
| 4 | human | 10125 | RASGRP1 | RAS guanyl releasing protein 1 | XM_005254114.3 | 93.6% | 93.6% | 1719_1720ins153 |
| 5 | human | 10125 | RASGRP1 | RAS guanyl releasing protein 1 | XM_011521151.3 | 79% | 78.2% | (many diffs) |
| 6 | human | 10125 | RASGRP1 | RAS guanyl releasing protein 1 | NM_001306086.2 | 74.6% | 73.9% | (many diffs) |
| 7 | mouse | 19419 | Rasgrp1 | RAS guanyl releasing protein 1 | NM_011246.2 | 86.7% | 90.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2463
- ORF length:
- 2391
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcacc ctgggcaagg cgagagaggc tccgcggaaa ccttcccatg 121 gctgcagagc tgcctctaaa gcaagactag aggcaaagcc agccaacagc cccttcccct 181 cccatcccag cttggcccac atcacccagt tccgaatgat ggtgtctctg ggacatttag 241 ccaaaggagc cagcctggac gatctcattg acagctgcat tcaatctttt gatgcagatg 301 gaaacctgtg tcgaagtaac caactgttgc aagtcatgct gaccatgcac cgaattgtca 361 tctcctctgc agaactgctc caaaaagtta tcaccctcta taaggatgct ttggcaaaga 421 attcaccagg actttgcctg aagatctgtt attttgtaag gtattggata acagaattct 481 gggtcatgtt taaaatggac gccagcttga cagacactat ggaggagttt caggaactgg 541 tgaaagctaa gggtgaggag ttacattgcc gcctgattga cacaactcaa atcaatgccc 601 gtgactggtc caggaaactt actcaaagga taaaatcaaa taccagcaag aaacggaaag 661 tctccctgct ctttgaccat ctggaaccag aagagctatc cgagcacctc acctaccttg 721 agttcaagtc tttccggagg atatcgttct ctgattatca gaattacctt gtaaatagct 781 gtgtgaagga aaaccccacc atggagcgat ctattgctct gtgcaacggc atctcccagt 841 gggtacaact gatggttctc agccgcccca cgccgcagct ccgagcagaa gtcttcatca 901 agttcatcca ggtggctcag aagctccacc aactacagaa cttcaataca ctgatggctg 961 tgataggtgg gctgtgtcac agctcaatct cgaggctcaa ggagacaagt tcgcatgtcc 1021 cacatgaaat caataaggtt ctcggtgaga tgactgagct gctgtcctcc tccagaaact 1081 acgacaatta ccggcgagcc tatggagagt gcaccgactt caagatcccc attctgggtg 1141 tgcatctcaa ggacctcatc tccctgtatg aagccatgcc tgactatctg gaggacggga 1201 aagtgaacgt ccataagcta ctggccctat acaatcatat cagtgaattg gtccagctgc 1261 aagaggtggc cccacccttg gaggctaaca aggacttggt acacttgctg acgttatccc 1321 tggatcttta ctacactgag gatgaaatct atgagctttc ctatgcccgg gaaccaagga 1381 accacagagc tccaccacta acaccttcaa agccaccagt agtagtggac tgggcttctg 1441 gagtgtctcc caaacctgat ccaaaaacca ttagcaaaca cgtccagagg atggtggatt 1501 ctgtcttcaa gaactatgat cacgaccagg atggatacat ttctcaggaa gaatttgaaa 1561 agattgctgc gagttttcca ttttccttct gtgtgatgga caaagacagg gaaggcctca 1621 tcagcaggga tgagatcaca gcctacttca tgagagccag ctcaatctat tccaagctgg 1681 gcctgggctt tcctcacaac ttccaagaga ccacctacct gaagcccact ttttgtgaca 1741 actgtgctgg atttctctgg ggagtgatca aacaaggata tcgatgtaaa gactgcggga 1801 tgaactgtca caaacaatgc aaagatctgg ttgtgtttga gtgtaagaag cgagccaaga 1861 acccagtagc tcccacagag aacaacactt ctgtggggcc agtgtccaac ctttgctcat 1921 tgggagccaa agatctgctc catgcacctg aggaaggacc ttttacattc cctaatgggg 1981 aggctgtgga acatggtgag gagagtaagg atcggaccat catgctgatg ggagtgtcct 2041 cacagaagat ttctcttcgg ctgaagaggg ctgttgccca caaggccacc cagactgaat 2101 cacagccttg gattggCAGT GAGGGCCCTT CAGGTCCCTT TGTGCTGTCT TCCCCAAGGA 2161 AGACAGCCCA GGATACTCTA TATGTGCTTC CCAGTCCCAC CTCTCCATGT CCTAGCCCAG 2221 TCTTGGTCAG AAAGCGGGCT TTTGTCAAGT GGGAGAATAA AGACTCCCTC ATAAAATCAA 2281 AGGAGGAGCT CCGTCACCTC AGACTGCCTA CCTACCAAGA ACTGGAACAG GAAATAAATA 2341 CTCTGAAAGC AGATAATGAT GCCCTAAAGA TCCAACTGAA ATATGCACAG AAGAAAATAG 2401 AATCCCTCCA GCTTGAAAAA AGCAATCATG TCTTAGCTCA AATGGAGCAG GGTGACTGTT 2461 CTTAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2521 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2581 CTTGGCCTTA TATATCTTGT GGAAAGGACG ATATCATGCT GACCCAAGCC CCCGGACGCG 2641 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt