Construct: ORF TRCN0000489634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021970.1_s317c1
- DNA Barcode:
- TACGATAGGAAGGGGGCTCTCGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DVL2 (1856)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1856 | DVL2 | dishevelled segment polarit... | NM_004422.3 | 100% | 100% | |
| 2 | human | 1856 | DVL2 | dishevelled segment polarit... | XM_005256502.2 | 99.4% | 99.3% | 508_509insGTGAGCATGGCG |
| 3 | human | 1856 | DVL2 | dishevelled segment polarit... | XR_001752443.2 | 78.9% | (many diffs) | |
| 4 | human | 1856 | DVL2 | dishevelled segment polarit... | XR_001752442.2 | 71.6% | 1_282del;1826_1891del;2557_3083del | |
| 5 | human | 1856 | DVL2 | dishevelled segment polarit... | XR_001752441.2 | 63.6% | (many diffs) | |
| 6 | mouse | 13543 | Dvl2 | dishevelled segment polarit... | NM_007888.3 | 88.1% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2277
- ORF length:
- 2208
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgggtagc agcactgggg gcggtggggt tggggagacg aaggtgattt 121 accacctgga tgaggaagag actccctacc tggtgaagat ccctgtcccc gccgagcgca 181 tcaccctcgg cgatttcaag agcgtcctgc agcggcccgc gggcgccaag tactttttca 241 agtctatgga tcaggatttc ggggtggtga aggaagaaat ttcagatgac aacgcccgcc 301 tcccctgctt caacggaagg gtggtatcct ggctggtgtc ctcagataat ccccaacccg 361 agatggcccc tccagtccat gagcctcggg cagaactggc gcctccagcc ccacctttac 421 ctcctttgcc acccgagagg accagcggca ttggggactc aaggcctcca tccttccacc 481 ctaatgtgtc cagcagccat gagaatctgg agcctgagac agaaaccgag tcagtagtgt 541 cactgaggcg ggagcggcct cgcaggagag acagcagtga gcatggcgct gggggccaca 601 ggactggtgg cccctcaagg ctggagcgcc acctggccgg atacgagagc tcctctaccc 661 tcatgaccag cgagctggag agtaccagcc tgggggactc ggacgaggag gacaccatga 721 gcaggttcag cagctccacg gagcagagca gtgcctcccg cctccttaag cgccaccggc 781 ggcgaaggaa gcagaggcca ccccgcctgg agaggacgtc atccttcagc agcgtcacag 841 attccacaat gtctctcaat atcatcacag tcacgctaaa catggagaag tacaacttcc 901 tgggtatctc cattgttggc cagagcaatg agcggggaga cggaggcatc tacattggct 961 ccatcatgaa gggtggggct gtggcggccg acgggcgcat tgagccaggg gacatgcttt 1021 tgcaggtgaa tgacatgaac tttgagaaca tgagcaacga tgacgctgtg cgggtgctga 1081 gggacattgt gcacaagcct ggccccattg tgctgactgt ggccaagtgc tgggatccct 1141 ctcctcaggc ctatttcact ctcccccgaa atgagcccat ccagccaatt gaccctgctg 1201 cctgggtgtc ccattccgcg gctctgactg gcaccttccc agcctatcca ggttcctcct 1261 ccatgagcac cattacatct ggatcgtctt tgcctgatgg ctgtgaaggc cggggtctct 1321 ccgtccatac ggacatggca tcggtgacca aggccatggc agctccagag tctggactgg 1381 aagtccggga ccgcatgtgg ctcaagatca ccatccctaa tgcctttctg ggctcggatg 1441 tggttgactg gctctaccat cacgtggagg gctttcctga gcggcgggag gcccgcaagt 1501 atgccagcgg gctgctcaaa gcaggcctga tccgacacac cgtcaacaag atcaccttct 1561 ctgagcagtg ctattacgtc ttcggagacc tcagtggtgg ctgtgagagc tacctagtca 1621 acctgtctct caatgacaac gatggctcca gtggggcttc agaccaggat accctggctc 1681 ctctgcctgg ggccaccccc tggcccctgc tgcccacttt ctcctaccaa taccctgccc 1741 cacaccccta cagcccgcag cctccaccct accatgagct ttcatcttac acctatggtg 1801 ggggcagtgc cagcagccag catagtgagg gcagccggag cagtgggtcg acacggagtg 1861 atgggggggc agggcgcacg gggaggcccg aggagcgggc ccccgagtcc aagtccggca 1921 gtggcagtga gtctgagccc tccagccgag ggggcagccT TCGGCGGGGT GGGGAAGCAA 1981 GTGGGACTAG CGATGGGGGC CCTCCTCCAT CCAGAGGCTC AACTGGGGGT GCCCCTAATC 2041 TCCGAGCCCA CCCAGGGCTC CATCCCTATG GACCGCCCCC TGGCATGGCC CTCCCCTACA 2101 ACCCCATGAT GGTGGTCATG ATGCCCCCAC CTCCACCTCC AGTCCCTCCA GCAGTGCAGC 2161 CTCCGGGGGC CCCTCCAGTC AGAGACCTGG GCTCTGTGCC CCCAGAACTG ACAGCCAGCC 2221 GCCAAAGCTT CCACATGGCC ATGGGCAATC CCAGCGAGTT CTTTGTGGAT GTTATGTAGA 2281 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2341 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2401 TTTATATATC TTGTGGAAAG GACGATACGA TAGGAAGGGG GCTCTCGTCA CGCGTTAAGT 2461 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt