Construct: ORF TRCN0000489639

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021492.1_s317c1
DNA Barcode:
ATTGATGTCTGGGAAGTCTCCCAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
TPK1 (27010)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489639
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350879.1 100% 100%
2 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_022445.4 100% 100%
3 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_005249970.1 100% 100%
4 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516031.1 90.3% 90.3% 256_333del
5 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516032.2 90.3% 90.3% 256_333del
6 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516033.2 90.3% 90.3% 256_333del
7 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516034.2 90.3% 90.3% 256_333del
8 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350881.1 90% 86.4% (many diffs)
9 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516035.3 81.5% 78% (many diffs)
10 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011969.1 81.2% 73.4% (many diffs)
11 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011972.1 80.3% 76.9% (many diffs)
12 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001042482.1 79.8% 79.8% 354_355ins147
13 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350880.1 79.8% 79.8% 354_355ins147
14 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516040.2 79.5% 76.5% (many diffs)
15 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516043.1 72.1% 72.1% 256_333del;432_433ins147
16 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350882.1 69.7% 76.8% 0_1ins136;48_168del
17 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350883.1 69.7% 76.8% 0_1ins136;48_168del
18 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350884.2 69.7% 76.8% 0_1ins136;48_168del
19 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516039.2 68.5% 64.3% 0_1ins136;50_107del;178_255del
20 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011974.1 68.5% 64.3% 0_1ins136;50_107del;178_255del
21 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011975.1 68.5% 64.3% 0_1ins136;50_107del;178_255del
22 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011980.2 66.4% 65% (many diffs)
23 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011981.2 66.4% 65% (many diffs)
24 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516037.2 63.9% 69.7% 0_1ins136;48_168del;241_318del
25 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011970.1 63.9% 69.7% 0_1ins136;48_168del;241_318del
26 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011971.1 63.9% 69.7% 0_1ins136;48_168del;241_318del
27 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350885.1 56.3% 56.3% 0_1ins318
28 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350886.1 56.3% 56.3% 0_1ins318
29 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350887.1 56.3% 56.3% 0_1ins318
30 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350889.1 56.3% 56.3% 0_1ins318
31 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350893.1 56.3% 56.3% 0_1ins318
32 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350894.1 56.3% 56.3% 0_1ins318
33 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516047.2 56.3% 56.3% 0_1ins318
34 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516048.1 56.3% 56.3% 0_1ins318
35 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011982.1 56.3% 56.3% 0_1ins318
36 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446717.1 56.3% 56.3% 0_1ins318
37 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446715.1 52.4% 57.6% 0_1ins136;48_168del;339_340ins147
38 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350895.1 51.7% 51.4% 0_1ins351;2T>A
39 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446716.1 51.7% 51.4% 0_1ins351;2T>A
40 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516046.1 44.4% 43.8% (many diffs)
41 human 27010 TPK1 thiamin pyrophosphokinase 1 XR_001744630.1 35.4% (many diffs)
42 human 27010 TPK1 thiamin pyrophosphokinase 1 NR_146936.2 24.2% (many diffs)
43 human 27010 TPK1 thiamin pyrophosphokinase 1 NR_146934.1 23.7% (many diffs)
44 mouse 29807 Tpk1 thiamine pyrophosphokinase NM_013861.4 86.8% 89.3% (many diffs)
45 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506219.3 78.9% 81% (many diffs)
46 mouse 29807 Tpk1 thiamine pyrophosphokinase NM_001311111.1 70% 70.7% (many diffs)
47 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506218.3 67.2% 66.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
801
ORF length:
729
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggagcat gcctttaccc cgttggagcc cctgctttcc actgggaatt
121tgaagtactg ccttgtaatt cttaatcagc ctttggacaa ctattttcgt catctttgga
181acaaagctct tttaagagcc tgtgccgatg gaggtgccaa ccgcttatat gatatcaccg
241aaggagagag agaaagcttt ttgcctgaat tcatcaatgg agactttgat tctattaggc
301ctgaagtcag agaatactat gctactaagg gatgtgagct catttcaact cctgatcaag
361accacactga ctttactaag tgCCTTAAAA TGCTCCAAAA GAAGATAGAA GAAAAAGACT
421TAAAGGTTGA TGTGATCGTG ACACTGGGAG GCCTTGCTGG GCGTTTTGAC CAGATTATGG
481CATCTGTGAA TACCTTGTTC CAAGCGACTC ACATCACTCC TTTTCCAATT ATAATAATCC
541AAGAGGAATC GCTGATCTAC CTGCTCCAAC CAGGAAAGCA CAGGTTGCAT GTAGACACTG
601GAATGGAGGG TGATTGGTGT GGCCTTATTC CTGTTGGACA GCCTTGTATG CAGGTTACAA
661CCACAGGCCT CAAGTGGAAC CTCACAAATG ATGTGCTTGC TTTTGGAACA TTGGTCAGTA
721CTTCCAATAC CTACGACGGG TCTGGTGTTG TGACTGTGGA AACTGACCAC CCACTCCTCT
781GGACCATGGC CATCAAAAGC TAAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA
841AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
901TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TTGATGTCTG
961GGAAGTCTCC CACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1021att

Download FASTA (ORF) (Full)