Construct: ORF TRCN0000489657
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019652.1_s317c1
- DNA Barcode:
- CCTTTACAGACTCCCAAACAAGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR2T4 (127074)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489657
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127074 | OR2T4 | olfactory receptor family 2... | NM_001004696.1 | 99.9% | 99.7% | 1044_1045insG |
| 2 | human | 343563 | OR2T29 | olfactory receptor family 2... | NM_001004694.2 | 85.3% | 81.6% | (many diffs) |
| 3 | human | 401993 | OR2T5 | olfactory receptor family 2... | NM_001004697.2 | 85.1% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1122
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggac aacatcacct ggatggccag ccacactgga tggtcggatt 121 tcatcctgat gggactcttc agacaatcca aacatccaat ggccaatatc acctggatgg 181 ccaaccacac tggatggtcg gatttcatcc tgttgggact cttcagacaa tccaaacatc 241 cagcactact ttgtgtggtc atttttgtgg ttttcctgat ggcgttgtct ggaaatgctg 301 tcctgatcct tctgatacac tgtgacgccc acctccacac ccccatgtac tttttcatca 361 gtcaattgtc tctcatggac atggcgtaca tttctgtcac tgtgcccaag atgctcctgg 421 accaggtcat gggtgtgaat aagatctcag cccctgagtg tgggatgcag atgttcttct 481 acgtgacact agcaggttca gaatttttcc ttctagccac catggcctat gaccgctacg 541 tggccatctg ccatcctctc cgttaccctg tcctcatgaa ccatagggtg tgtctcttcc 601 tgtcatcagg ctgctggttc ctgggctcag tggatggctt cacattcact cccatcacca 661 tgaccttccc cttccgtgga tcccgggaga ttcatcattt cttctgtgaa gttcctgctg 721 tattgaaTCT CTCCTGCTCA GACACCTCAC TCTATGAGAT TTTCATGTAC TTGTGCTGTG 781 TCCTCATGCT CCTCATCCCT GTGGTGATCA TTTCAAGCTC CTATTTACTC ATCCTCCTCA 841 CCATCCACGG GATGAACTCA GCAGAGGGCC GGAAAAAGGC CTTTGCCACC TGCTCCTCCC 901 ACCTGACTGT GGTCATCCTC TTCTATGGGG CTGCCATCTA CACCTACATG CTCCCCAGCT 961 CCTACCACAC CCCTGAGAAG GACATGATGG TATCTGTCTT CTATACCATC CTCACTCCAG 1021 TGGTGAACCC TTTAATCTAT AGTCTTAGGA ATAAGGATGT CATGGGGGCT CTGAAGAAAA 1081 TGTTAACAGT GGAACCTGCC TTTCAAAAAG CTATGGAGGA CCCAGCTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGACCTTTA CAGACTCCCA AACAAGAGAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt