Construct: ORF TRCN0000489665
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021285.1_s317c1
- DNA Barcode:
- TTTTACCGAGTGAGAGTTTGTCTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RAB33A (9363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489665
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9363 | RAB33A | RAB33A, member RAS oncogene... | NM_004794.3 | 100% | 100% | |
| 2 | human | 9363 | RAB33A | RAB33A, member RAS oncogene... | XM_017029963.2 | 64.1% | 63.7% | (many diffs) |
| 3 | mouse | 19337 | Rab33a | RAB33A, member RAS oncogene... | NM_011228.2 | 92.2% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 783
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgcag cccatcctgg gccatgggag cctgcagccc gcctcggccg 121 ctggcctggc gtccctggag ctcgactcgt cgctggacca gtacgtgcag attcgcatct 181 tcaaaataat cgtgattggg gactccaacg tgggcaagac ctgcctgacc ttccgcttct 241 gcgggggtac cttcccagac aagactgaag ccaccatcgg cgtggacttc agggagaaga 301 ccgtggaaat cgagggcgag aagatcaagg ttcaggtgtg ggacacagca ggTCAGGAAC 361 GTTTCCGCAA AAGCATGGTC GAGCATTACT ACCGCAACGT ACATGCCGTG GTCTTCGTCT 421 ATGACGTCAC CAAGATGACA TCTTTCACCA ACCTCAAAAT GTGGATCCAA GAATGCAATG 481 GGCATGCTGT GCCCCCACTA GTCCCCAAAG TGCTTGTGGG CAACAAGTGT GACTTGAGGG 541 AACAGATCCA GGTGCCCTCC AACTTAGCCC TGAAATTTGC TGATGCCCAC AACATGCTCT 601 TGTTTGAGAC ATCGGCCAAG GACCCCAAAG AGAGCCAGAA CGTGGAGTCG ATTTTCATGT 661 GCTTGGCTTG CCGATTGAAG GCCCAGAAAT CCCTGCTGTA TCGTGATGCT GAGAGGCAGC 721 AGGGGAAGGT GCAGAAACTG GAGTTCCCAC AGGAAGCTAA CAGTAAAACT TCCTGTCCTT 781 GTTAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 841 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 901 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTTTACCGA GTGAGAGTTT GTCTAACGCG 961 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt