Construct: ORF TRCN0000489667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020607.1_s317c1
- DNA Barcode:
- GTATAACCCGCCAGGCTTGTTATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EPHB1 (2047)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2047 | EPHB1 | EPH receptor B1 | NM_004441.5 | 100% | 100% | |
| 2 | human | 2047 | EPHB1 | EPH receptor B1 | XM_017005866.2 | 94.7% | 94.7% | 1297_1458del |
| 3 | human | 2047 | EPHB1 | EPH receptor B1 | XM_017005867.1 | 93.2% | 92.8% | (many diffs) |
| 4 | human | 2047 | EPHB1 | EPH receptor B1 | XM_024453389.1 | 92.6% | 92.6% | 0_1ins66;1231_1392del |
| 5 | human | 2047 | EPHB1 | EPH receptor B1 | XM_024453390.1 | 92.6% | 92.6% | 0_1ins66;1231_1392del |
| 6 | mouse | 270190 | Ephb1 | Eph receptor B1 | NM_173447.3 | 91.3% | 98.8% | (many diffs) |
| 7 | mouse | 270190 | Ephb1 | Eph receptor B1 | NM_001168296.1 | 87.3% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3024
- ORF length:
- 2952
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccctg gattatctac tactgctcct cctggcatcc gcagtggctg 121 cgatggaaga aacgttaatg gacaccagaa cggctactgc agagctgggc tggacggcca 181 atcctgcgtc cgggtgggaa gaagtcagtg gctacgatga aaacctgaac accatccgca 241 cctaccaggt gtgcaatgtc ttcgagccca accagaacaa ttggctgctc accaccttca 301 tcaaccggcg gggggcccat cgcatctaca cagagatgcg cttcactgtg agagactgca 361 gcagcctccc taatgtccca ggatcctgca aggagacctt caacttgtat tactatgaga 421 ctgactctgt cattgccacc aagaagtcag ccttctggtc tgaggccccc tacctcaaag 481 tagacaccat tgctgcagat gagagcttct cccaggtgga ctttggggga aggctgatga 541 aggtaaacac agaagtcagg agctttgggc ctcttactcg gaatggtttt tacctcgctt 601 ttcaggatta tggagcctgt atgtctcttc tttctgtccg tgtcttcttc aaaaagtgtc 661 ccagcattgt gcaaaatttt gcagtgtttc cagagactat gacaggggca gagagcacat 721 ctctggtgat tgctcggggc acatgcatcc ccaacgcaga ggaagtggac gtgcccatca 781 aactctactg caacggggat ggggaatgga tggtgcctat tgggcgatgc acctgcaagc 841 ctggctatga gcctgagaac agcgtggcat gcaaggcttg ccctgcaggg acattcaagg 901 ccagccagga agctgaaggc tgctcccact gcccctccaa cagccgctcc cctgcagagg 961 cgtctcccat ctgcacctgt cggaccggtt attaccgagc ggactttgac cctccagaag 1021 tggcatgcac tagcgtccca tcaggtcccc gcaatgttat ctccatcgtc aatgagacgt 1081 ccatcattct ggagtggcac cctccaaggg agacaggtgg gcgggatgat gtgacctaca 1141 acatcatctg caaaaagtgc cgggcagacc gccggagctg ctcccgctgt gacgacaatg 1201 tggagtttgt gcccaggcag ctgggcctga cggagtgccg cgtctccatc agcagcctgt 1261 gggcccacac cccctacacc tttgacatcc aggccatcaa tggagtctcc agcaagagtc 1321 ccttcccccc acagcacgtc tctgtcaaca tcaccacaaa ccaagccgcc ccctccaccg 1381 ttcccatcat gcaccaagtc agtgccacta tgaggagcat caccttgtca tggccacagc 1441 cggagcagcc caatggcatc atcctggact atgagatccg gtactatgag aaggaacaca 1501 atgagttcaa ctcctccatg gccaggagtc agaccaacac agcaaggatt gatgggctgc 1561 ggcctggcat ggtatatgtg gtacaggtgc gtgcccgcac tgttgctggc tacggcaagt 1621 tcagtggcaa gatgtgcttc cagactctga ctgacgatga ttacaagtca gagctgaggg 1681 agcagctgcc cctgattgct ggctcggcag cggccggggt cgtgttcgtt gtgtccttgg 1741 tggccatctc tatcgtctgt agcaggaaac gggcttatag caaagaggct gtgtacagcg 1801 ataagctcca gcattacagc acaggccgag gctccccagg gatgaagatc tacattgacc 1861 ccttcactta cgaggatccc aacgaagctg tccgggagtt tgccaaggag attgatgtat 1921 cttttgtgaa aattgaagag gtcatcggag caggggagtt tggagaagtg tacaaggggc 1981 gtttgaaact gccaggcaag agggaaatct acgtggccat caagaccctg aaggcagggt 2041 actcggagaa gcagcgtcgg gactttctga gtgaggcgag catcatgggc cagttcgacc 2101 atcctaacat cattcgcctg gagggtgtgg tcaccaagag tcggcctgtc atgatcatca 2161 cagagttcat ggagaatggt gcattggatt ctttcctcag gcaaaatgac gggcagttca 2221 ccgtgatcca gcttgtgggt atgctcaggg gcatcgctgc tggcatgaag tacctggctg 2281 agatgaatta tgtgcatcgg gacctggctg ctaggaacat tctggtcaac agtaacctgg 2341 tgtgcaaggt gtccgacttt ggcctctccc gctacctcca ggatgacacc tcagatccca 2401 cctacaccag ctccttggga gggaagatcc ctgtgagatg gacagctcca gaggccatcg 2461 cctaccgcaa gttcacttca gccagcgacg tttggagcta tgggatcgtc atgtgggaag 2521 tcatgtcatt tggagagaga ccctattggg atatgtccaa ccaagatgtc atcaatgcca 2581 tcgagcagga ctaccggctg cccccaccca tggactgtcc agctgctcta caccagctca 2641 tgctggactg ttggcagaag gaccggaaca gccggccccg gtttgcggag attgtcaaca 2701 ccctagataa gatgatccgg aacccggcaa gtctcaagac tgtggcaacc atcaccgccg 2761 tgccttccca gccccTGCTC GACCGCTCCA TCCCAGACTT CACGGCCTTT ACCACCGTGG 2821 ATGACTGGCT CAGCGCCATC AAAATGGTCC AGTACAGGGA CAGCTTCCTC ACTGCTGGCT 2881 TCACCTCCCT CCAGCTGGTC ACCCAGATGA CATCAGAAGA CCTCCTGAGA ATAGGCATCA 2941 CCTTGGCAGG CCATCAGAAG AAGATCCTGA ACAGCATTCA TTCTATGAGG GTCCAGATAA 3001 GTCAGTCACC AACGGCAATG GCATAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3061 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3121 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTATAACC 3181 CGCCAGGCTT GTTATGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3241 aagatt