Construct: ORF TRCN0000489686
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020892.1_s317c1
- DNA Barcode:
- AAGACGGACTACGCCATATATGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HTR5A (3361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489686
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3361 | HTR5A | 5-hydroxytryptamine recepto... | NM_024012.3 | 99.9% | 100% | 12T>A |
| 2 | mouse | 15563 | Htr5a | 5-hydroxytryptamine (seroto... | NM_008314.2 | 84.3% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1143
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggattta ccagtgaacc taacctcctt ttccctctcc accccctccc 121 ctttggagac caaccacagc ctcggcaaag acgacctgcg ccccagctcg cccctgctct 181 cggtcttcgg agtgcttatt ctcaccttgc tgggctttct ggtggcggcg acgttcgcct 241 ggaacctgct ggtgctggcg accatcctcc gtgtacgcac cttccaccgc gtgccccaca 301 acctggtggc atccatggcc gtctcggatg tcctggtggc cgcgctggtc atgccgctga 361 gcctggtgca cgagctgtcc gggcgccgct ggcagctagg tcggaggctg tgccagcttt 421 ggatcgcgtg cgacgtgctt tgctgcacgg ccagcatctg gaacgtgacg gccatagccc 481 tggaccgcta ctggtccatc acgcgccaca tggaatacac gctccgcacc cgcaagtgcg 541 tctccaacgt catgatcgcg ctcacctggg cactctccgc tgtcatctct ctggccccgc 601 tgctttttgg ctggggagag acgtactctg agggcagcga ggagtgccag gtaagccgcg 661 agccttccta cgccgtgttc tccaccgtag gcgccttcta cctgccgctc tgtGTGGTGC 721 TCTTCGTGTA CTGGAAGATC TACAAGGCTG CCAAGTTCCG CGTGGGCTCC AGGAAGACCA 781 ATAGCGTCTC ACCCATATCC GAAGCTGTGG AGGTGAAGGA CTCTGCCAAA CAGCCCCAGA 841 TGGTGTTCAC GGTCCGCCAC GCCACCGTCA CCTTCCAGCC AGAAGGGGAC ACGTGGCGGG 901 AGCAGAAGGA GCAGCGGGCC GCCCTCATGG TGGGCATCCT CATTGGCGTG TTCGTGCTCT 961 GCTGGATCCC CTTCTTTCTC ACCGAGCTCA TCAGTCCCCT CTGCTCCTGT GACATCCCCG 1021 CCATCTGGAA AAGCATCTTC CTGTGGCTTG GCTACTCCAA CTCCTTCTTT AACCCCCTGA 1081 TCTATACGGC TTTCAACAAG AACTACAACA GCGCCTTCAA GAACTTCTTT TCTAGGCAAC 1141 ACTAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1201 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1261 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAAGACGGAC TACGCCATAT ATGATACGCG 1321 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt