Construct: ORF TRCN0000489692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020021.1_s317c1
- DNA Barcode:
- TGCTGGAACGATACCCATGTTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSNK1E (1454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102800317 | TPTEP2-CSNK1E | TPTEP2-CSNK1E readthrough | NM_001289912.2 | 99.9% | 99.7% | 1248_1249insG |
| 2 | human | 1454 | CSNK1E | casein kinase 1 epsilon | NM_001894.4 | 99.9% | 99.7% | 1248_1249insG |
| 3 | human | 1454 | CSNK1E | casein kinase 1 epsilon | NM_152221.3 | 99.9% | 99.7% | 1248_1249insG |
| 4 | human | 1453 | CSNK1D | casein kinase 1 delta | NM_139062.4 | 77.9% | 84.1% | (many diffs) |
| 5 | human | 1453 | CSNK1D | casein kinase 1 delta | NM_001363749.2 | 74.1% | 78.6% | (many diffs) |
| 6 | human | 1453 | CSNK1D | casein kinase 1 delta | XM_005256336.4 | 70.5% | 74.5% | (many diffs) |
| 7 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | NM_001289898.1 | 90.6% | 98.5% | (many diffs) |
| 8 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | NM_013767.6 | 90.6% | 98.5% | (many diffs) |
| 9 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_017316657.1 | 90.6% | 98.5% | (many diffs) |
| 10 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_017316658.1 | 90.6% | 98.5% | (many diffs) |
| 11 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_017316659.1 | 90.6% | 98.5% | (many diffs) |
| 12 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_006521043.3 | 85.3% | 67.9% | (many diffs) |
| 13 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245649.1 | 85.3% | 67.9% | (many diffs) |
| 14 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245650.2 | 85.3% | 67.9% | (many diffs) |
| 15 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245651.1 | 85.3% | 67.9% | (many diffs) |
| 16 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245652.2 | 85.3% | 67.9% | (many diffs) |
| 17 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245653.2 | 85.3% | 67.9% | (many diffs) |
| 18 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_011245654.2 | 85.3% | 67.9% | (many diffs) |
| 19 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | NM_001289899.1 | 75.4% | 73.1% | (many diffs) |
| 20 | mouse | 27373 | Csnk1e | casein kinase 1, epsilon | XM_017316660.1 | 75.4% | 73.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1320
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagctacgt gtggggaaca agtaccgcct gggacggaag atcgggagcg 121 ggtccttcgg agatatctac ctgggtgcca acatcgcctc tggtgaggaa gtcgccatca 181 agctggagtg tgtgaagaca aagcaccccc agctgcacat cgagagcaag ttctacaaga 241 tgatgcaggg tggcgtgggg atcccgtcca tcaagtggtg cggagctgag ggcgactaca 301 acgtgatggt catggagctg ctggggccta gcctcgagga cctgttcaac ttctgttccc 361 gcaaattcag cctcaagacg gtgctgctct tggccgacca gatgatcagc cgcatcgagt 421 atatccactc caagaacttc atccaccggg acgtcaagcc cgacaacttc ctcatggggc 481 tggggaagaa gggcaacctg gtctacatca tcgacttcgg cctggccaag aagtaccggg 541 acgcccgcac ccaccagcac attccctacc gggaaaacaa gaacctgacc ggcacggccc 601 gctacgcttc catcaacacg cacctgggca ttgagcaaag ccgtcgagat gacctggaga 661 gcctgggcta cgtgctcatg tacttcaacc tgggctccct gccctggcag gggctcaaag 721 cagccaccaa gcgccagaag tatgaacgga tcagcgagaa gaagatgtca acgcccatcg 781 aggtcctctg caaaggctat ccctccgaat tctcaacata cctcaacttc tgccgctccc 841 tgcggtttga cgacaagccc gactactctt acctacgtca gctcttccgc aacctcttcc 901 accggcaggg cttctcctat GACTACGTCT TTGACTGGAA CATGCTGAAA TTCGGTGCAG 961 CCCGGAATCC CGAGGATGTG GACCGGGAGC GGCGAGAACA CGAACGCGAG GAGAGGATGG 1021 GGCAGCTACG GGGGTCCGCG ACCCGAGCCC TGCCCCCTGG CCCACCCACG GGGGCCACTG 1081 CCAACCGGCT CCGCAGTGCC GCCGAGCCCG TGGCTTCCAC GCCAGCCTCC CGCATCCAGC 1141 CGGCTGGCAA TACTTCTCCC AGAGCGATCT CGCGGGTCGA CCGGGAGAGG AAGGTGAGTA 1201 TGAGGCTGCA CAGGGGTGCG CCCGCCAACG TCTCCTCCTC AGACCTCACT GGGCGGCAAG 1261 AGGTCTCCCG GATCCCAGCC TCACAGACAA GTGTGCCATT TGACCATCTC GGGAAGGACC 1321 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GATGCTGGAA CGATACCCAT GTTATCACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt