Construct: ORF TRCN0000489697
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019913.1_s317c1
- DNA Barcode:
- ACCTTGCTCTTAACTTGACGTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHKG2 (5261)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489697
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5261 | PHKG2 | phosphorylase kinase cataly... | NM_000294.3 | 99.9% | 99.7% | 1218_1219insG |
| 2 | human | 5261 | PHKG2 | phosphorylase kinase cataly... | NM_001172432.1 | 90.5% | 88.5% | (many diffs) |
| 3 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | NM_026888.3 | 89.5% | 93.3% | (many diffs) |
| 4 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508167.3 | 89.5% | 93.3% | (many diffs) |
| 5 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_011241891.1 | 89.5% | 93.3% | (many diffs) |
| 6 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508168.2 | 67.5% | 69.2% | (many diffs) |
| 7 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508169.3 | 67.5% | 69.2% | (many diffs) |
| 8 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508170.2 | 66.6% | 69.7% | (many diffs) |
| 9 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508171.2 | 66.6% | 69.7% | (many diffs) |
| 10 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508172.3 | 64.8% | 68% | (many diffs) |
| 11 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_017312279.1 | 64.8% | 68% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1290
- ORF length:
- 1221
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gacgctggac gtggggccgg aggatgagct gcccgactgg gccgccgcca 121 aagagtttta ccagaagtac gaccctaagg acgtcatcgg cagaggagtg agctctgtgg 181 tccgccgttg tgttcatcga gctactggcc acgagtttgc ggtgaagatt atggaagtga 241 cagctgagcg gctgagtcct gagcagctgg aggaggtgcg ggaagccaca cggcgagaga 301 cacacatcct tcgccaggtc gccggccacc cccacatcat caccctcatc gattcctacg 361 agtcttctag cttcatgttc ctggtgtttg acctgatgcg gaagggagag ctgtttgact 421 atctcacaga gaaggtggcc ctctctgaaa aggaaaccag gtccatcatg cggtctctgc 481 tggaagcagt gagctttctc catgccaaca acattgtgca tcgagatctg aagcccgaga 541 atattctcct agatgacaat atgcagatcc gactttcaga tttcgggttc tcctgccact 601 tggaacctgg cgagaagctt cgagagttgt gtgggacccc agggtatcta gcgccagaga 661 tccttaaatg ctccatggat gaaacccacc caggctatgg caaggaggtc gacctctggg 721 cctgtggggt gatcttgttc acactcctgg ctggctcgcc acccttctgg caccggcggc 781 agatcctgat gttacgcatg atcatggagg gccagtacca gttcagttcc cccgagtggg 841 atgaccgttc cagcactgtc aaagacctga tctccaggct gctgcaggtg gatcctgagg 901 cacgcctgac agctgagcag gcccTACAGC ACCCCTTCTT TGAGCGTTGT GAAGGCAGCC 961 AACCCTGGAA CCTCACCCCC CGCCAGCGGT TCCGGGTGGC AGTGTGGACA GTGCTGGCTG 1021 CTGGACGAGT GGCCCTAAGC ACCCATCGTG TACGGCCACT GACCAAGAAT GCACTGTTGA 1081 GGGACCCTTA TGCGCTGCGG TCAGTGCGGC ACCTCATCGA CAACTGTGCC TTCCGGCTCT 1141 ACGGGCACTG GGTAAAGAAA GGGGAGCAGC AGAACCGGGC GGCTCTCTTT CAGCACCGGC 1201 CCCCTGGGCC TTTTCCCATC ATGGGCCCTG AAGAGGAGGG AGACTCTGCT GCTATAACTG 1261 AGGATGAGGC CGTGCTTGTG CTGGGCGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCTTGCT 1441 CTTAACTTGA CGTGTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt