Construct: ORF TRCN0000489710
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020596.1_s317c1
- DNA Barcode:
- TGTCAATGGTAATAGGAATCCGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- IRAK1 (3654)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489710
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001569.4 | 100% | 100% | |
| 2 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001025242.2 | 95.7% | 95.7% | 1536_1537ins90 |
| 3 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | XM_005274668.4 | 92.4% | 92.4% | 136_213del;1614_1615ins90 |
| 4 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001025243.2 | 88.9% | 88.9% | 1301_1302ins237 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2208
- ORF length:
- 2136
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccggg gggccgggcc cgggggagcc cgcagccccc ggcgcccagc 121 acttcttgta cgaggtgccg ccctgggtca tgtgccgctt ctacaaagtg atggacgccc 181 tggagcccgc cgactggtgc cagttcgccg ccctgatcgt gcgcgaccag accgagctgc 241 ggctgtgcga gcgctccggg cagcgcacgg ccagcgtcct gtggccctgg atcaaccgca 301 acgcccgtgt ggccgacctc gtgcacatcc tcacgcacct gcagctgctc cgtgcgcggg 361 acatcatcac agcctggcac cctcccgccc cgcttccgtc cccaggcacc actgccccga 421 ggcccagcag catccctgca cccgccgagg ccgaggcctg gagcccccgg aagttgccat 481 cctcagcctc caccttcctc tccccagctt ttccaggctc ccagacccat tcagggcctg 541 agctcggcct ggtcccaagc cctgcttccc tgtggcctcc accgccatct ccagcccctt 601 cttctaccaa gccaggccca gagagctcag tgtccctcct gcagggagcc cgcccctttc 661 cgttttgctg gcccctctgt gagatttccc ggggcaccca caacttctcg gaggagctca 721 agatcgggga gggtggcttt gggtgcgtgt accgggcggt gatgaggaac acggtgtatg 781 ctgtgaagag gctgaaggag aacgctgacc tggagtggac tgcagtgaag cagagcttcc 841 tgaccgaggt ggagcagctg tccaggtttc gtcacccaaa cattgtggac tttgctggct 901 actgtgctca gaacggcttc tactgcctgg tgtacggctt cctgcccaac ggctccctgg 961 aggaccgtct ccactgccag acccaggcct gcccacctct ctcctggcct cagcgactgg 1021 acatccttct gggtacagcc cgggcaattc agtttctaca tcaggacagc cccagcctca 1081 tccatggaga catcaagagt tccaacgtcc ttctggatga gaggctgaca cccaagctgg 1141 gagactttgg cctggcccgg ttcagccgct ttgccgggtc cagccccagc cagagcagca 1201 tggtggcccg gacacagaca gtgcggggca ccctggccta cctgcccgag gagtacatca 1261 agacgggaag gctggctgtg gacacggaca ccttcagctt tggggtggta gtgctagaga 1321 ccttggctgg tcagagggct gtgaagacgc acggtgccag gaccaagtat ctgaaagacc 1381 tggtggaaga ggaggctgag gaggctggag tggctttgag aagcacccag agcacactgc 1441 aagcaggtct ggctgcagat gcctgggctg ctcccatcgc catgcagatc tacaagaagc 1501 acctggaccc caggcccggg ccctgcccac ctgagctggg cctgggcctg ggccagctgg 1561 cctgctgctg cctgcaccgc cgggccaaaa ggaggcctcc tatgacccag gtgtacgaga 1621 ggctagagaa gctgcaggca gtggtggcgg gggtgcccgg gcattcggag gccgccagct 1681 gcatcccccc ttccccgcag gagaactcct acgtgtccag cactggcaga gcccacagtg 1741 gggctgctcc atggcagccc ctggcagcgc catcaggagc cagtgcccag gcagcagagc 1801 agctgcagag aggccccaac cagcccgtgg agagtgacga gagcctaggc ggcctctctg 1861 ctgccctgcg ctcctggcac ttgactccaa gctgccctct ggacccagca cccctcaggg 1921 aggccggctg tccTCAGGGG GACACGGCAG GAGAATCGAG CTGGGGGAGT GGCCCAGGAT 1981 CCCGGCCCAC AGCCGTGGAA GGACTGGCCC TTGGCAGCTC TGCATCATCG TCGTCAGAGC 2041 CACCGCAGAT TATCATCAAC CCTGCCCGAC AGAAGATGGT CCAGAAGCTG GCCCTGTACG 2101 AGGATGGGGC CCTGGACAGC CTGCAGCTGC TGTCGTCCAG CTCCCTCCCA GGCTTGGGCC 2161 TGGAACAGGA CAGGCAGGGG CCCGAAGAAA GTGATGAATT TCAGAGCTGA GACCCAGCTT 2221 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2281 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2341 CTTGTGGAAA GGACGATGTC AATGGTAATA GGAATCCGTG ACGCGTTAAG TCgacaatca 2401 acctctggat tacaaaattt gtgaaagatt