Construct: ORF TRCN0000489716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019197.1_s317c1
- DNA Barcode:
- CGAGGCGCCGCAGTTACAATTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR139 (124274)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124274 | GPR139 | G protein-coupled receptor 139 | NM_001002911.3 | 93.9% | 93.8% | 1046_1047insA;1053_1057delATCCC;1059_1060ins62 |
| 2 | human | 124274 | GPR139 | G protein-coupled receptor 139 | NM_001318483.1 | 69% | 68.9% | (many diffs) |
| 3 | mouse | 209776 | Gpr139 | G protein-coupled receptor 139 | NM_001024138.1 | 82.6% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1194
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag cacacgcacg cccacctcgc agccaacagc tcgctgtctt 121 ggtggtcccc cggctcggcc tgcggcttgg gtttcgtgcc cgtggtctac tacagcctct 181 tgctgtgcct cggtttacca gcaaatatct tgacagtgat catcctctcc cagctggtgg 241 caagaagaca gaagtcctcc tacaactatc tcttggcact cgctgctgcc gacatcttgg 301 tcctcttttt catagtgttt gtggacttcc tgttggaaga tttcatcttg aacatgcaga 361 tgcctcaggt ccccgacaag atcatagaag tgctggaatt ctcatccatc cacacctcca 421 tatggattac tgtaccgtta accattgaca ggtatatcgc tgtctgccac ccgctcaagt 481 accacacggt ctcataccca gcccgcaccc ggaaagtcat tgtaagtgtt tacatcacct 541 gcttcctgac cagcatcccc tattactggt ggcccaacat ctggactgaa gactacatca 601 gcacctctgt gcatcacgtc ctcatctgga tccactgctt caccgtctac ctggtgccct 661 gctccatctt cttcatcttg aactcaatca ttgtgtacaa gctcaggagg aagagcaatt 721 ttcgtctccg tggctactcc acggggaaga ccaccgccat cttgttcacc attacctcca 781 tctttgccac actttGGGCC CCCCGCATCA TCATGATTCT TTACCACCTC TATGGGGCGC 841 CCATCCAGAA CCGCTGGCTG GTACACATCA TGTCCGACAT TGCCAACATG CTAGCCCTTC 901 TGAACACAGC CATCAACTTC TTCCTCTACT GCTTCATCAG CAAGCGGTTC CGCACCATGG 961 CAGCCGCCAC GCTCAAGGCT TTCTTCAAGT GCCAGAAGCA ACCTGTACAG TTCTACACCA 1021 ATCATAACTT TTCCATAACA AGTAGCCCCT GGATCTCGCC GGCAAACTCA CACTGCATCA 1081 AGATGCTGGT GTACCAGTAT GACAAAAATG GAAAACCTAT AAAAAGTCGT AATGACAGCA 1141 AAAGCTCCTA CCAGTTTGAA GATGCCATTG GAGCTTGTGT CATCATCCTG GACCCAGCTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGACGAG GCGCCGCAGT TACAATTATT ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt