Construct: ORF TRCN0000489733
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021300.1_s317c1
- DNA Barcode:
- CCGTTTTCCGCGAATTTCCTCTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ANAPC10 (10393)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489733
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NM_001256706.2 | 100% | 100% | |
| 2 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NM_001256707.1 | 100% | 100% | |
| 3 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NM_001256708.1 | 100% | 100% | |
| 4 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NM_014885.4 | 100% | 100% | |
| 5 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_011531524.3 | 100% | 100% | |
| 6 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_024453875.1 | 100% | 100% | |
| 7 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NM_001256709.1 | 94.3% | 94.3% | 1_33del |
| 8 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_017007640.2 | 94.3% | 94.3% | 1_33del |
| 9 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NM_001318367.1 | 84.4% | 84.4% | 1_102del |
| 10 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_017007639.2 | 84.4% | 84.4% | 1_102del |
| 11 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_024453874.1 | 82.9% | 82.9% | 1_114del |
| 12 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_017007642.1 | 64.5% | 57.3% | (many diffs) |
| 13 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NM_001256710.1 | 60.1% | 60.5% | (many diffs) |
| 14 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NM_001256711.1 | 60.1% | 60.5% | (many diffs) |
| 15 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_017007644.2 | 60.1% | 60.5% | (many diffs) |
| 16 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_011531532.2 | 59% | 58.9% | 328A>C;330_330delGins226 |
| 17 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_017007641.1 | 57.9% | 51.3% | (many diffs) |
| 18 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_011531525.2 | 56.9% | 50.4% | (many diffs) |
| 19 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_011531527.2 | 52.1% | 48.4% | (many diffs) |
| 20 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_024453876.1 | 49.9% | 50.2% | (many diffs) |
| 21 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_011531529.2 | 49% | 48.8% | 1_114del;442A>C;444_444delGins226 |
| 22 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NM_001256712.1 | 44.8% | 36.3% | 205_206ins121;249_250ins185 |
| 23 | human | 10393 | ANAPC10 | anaphase promoting complex ... | XM_011531531.3 | 37.2% | 30.2% | 1_114del;319_320ins121;363_364ins185 |
| 24 | human | 10393 | ANAPC10 | anaphase promoting complex ... | NR_046345.1 | 27.5% | (many diffs) | |
| 25 | mouse | 68999 | Anapc10 | anaphase promoting complex ... | NM_026904.2 | 92.6% | 99.4% | (many diffs) |
| 26 | mouse | 68999 | Anapc10 | anaphase promoting complex ... | XM_006531339.3 | 92.6% | 99.4% | (many diffs) |
| 27 | mouse | 68999 | Anapc10 | anaphase promoting complex ... | XM_006531340.3 | 92.6% | 99.4% | (many diffs) |
| 28 | mouse | 68999 | Anapc10 | anaphase promoting complex ... | XM_006531343.3 | 92.6% | 99.4% | (many diffs) |
| 29 | mouse | 68999 | Anapc10 | anaphase promoting complex ... | XM_006531341.3 | 54.7% | 58.3% | (many diffs) |
| 30 | mouse | 68999 | Anapc10 | anaphase promoting complex ... | XM_006531342.1 | 54.7% | 58.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 627
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgactaca ccaaacaaga cacctcctgg tgctgacccc aagcagttgg 121 aaaggactgg aacagtacgg gaaattgggt cacaagctgt ttggtcactc tcatcttgca 181 aaccaggatt tggagtggat cagttacgag atgacaatct agaaacttat tggcaatcag 241 atggttccca gcctcattta gtgaaCATCC AATTCAGAAG AAAAACAACA GTGAAGACAT 301 TATGTATTTA TGCAGACTAC AAATCTGATG AAAGCTATAC TCCAAGCAAG ATCTCAGTCA 361 GAGTAGGAAA TAATTTTCAC AACCTTCAAG AAATTCGGCA ACTTGAGTTG GTGGAACCAA 421 GTGGCTGGAT TCATGTTCCC TTAACTGACA ATCATAAGAA GCCAACTCGT ACATTCATGA 481 TACAGATTGC TGTTCTAGCC AATCACCAGA ATGGAAGAGA CACCCATATG AGACAAATTA 541 AAATATACAC ACCAGTAGAA GAGAGCTCCA TTGGTAAATT TCCTAGATGT ACAACTATAG 601 ATTTCATGAT GTATCGTTCA ATAAGGTAGA ACCCAGCTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCGTT 781 TTCCGCGAAT TTCCTCTGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt