Transcript: Human XM_011531531.3

PREDICTED: Homo sapiens anaphase promoting complex subunit 10 (ANAPC10), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANAPC10 (10393)
Length:
1362
CDS:
15..380

Additional Resources:

NCBI RefSeq record:
XM_011531531.3
NBCI Gene record:
ANAPC10 (10393)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011531531.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000004433 ACAAGGCATCCGTTATATCTA pLKO.1 645 3UTR 100% 5.625 7.875 N ANAPC10 n/a
2 TRCN0000010876 TGGAGTGGATCAGTTACGAGA pLKO.1 248 CDS 100% 2.640 3.696 N ANAPC10 n/a
3 TRCN0000315145 CCCTTAACTGACAATCATAAG pLKO_005 374 CDS 100% 10.800 7.560 N ANAPC10 n/a
4 TRCN0000315080 GTACATTCATGATACAGATTG pLKO_005 405 3UTR 100% 10.800 7.560 N ANAPC10 n/a
5 TRCN0000315081 TTCTAGCCAATCACCAGAATG pLKO_005 429 3UTR 100% 10.800 7.560 N ANAPC10 n/a
6 TRCN0000004434 GACAATCATAAGAAGCCAACT pLKO.1 383 3UTR 100% 4.050 2.835 N ANAPC10 n/a
7 TRCN0000004431 AGTACGGGAAATTGGGTCACA pLKO.1 191 CDS 100% 2.640 1.848 N ANAPC10 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011531531.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02418 pDONR223 100% 37.2% 30.2% None 1_114del;319_320ins121;363_364ins185 n/a
2 ccsbBroad304_02418 pLX_304 0% 37.2% 30.2% V5 1_114del;319_320ins121;363_364ins185 n/a
3 TRCN0000473829 CATAGCCTTGAACAAGGAGAACCC pLX_317 94.4% 37.2% 30.2% V5 1_114del;319_320ins121;363_364ins185 n/a
4 TRCN0000489733 CCGTTTTCCGCGAATTTCCTCTGT pLX_317 64.2% 37.2% 30.2% V5 (not translated due to prior stop codon) 1_114del;319_320ins121;363_364ins185 n/a
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