Construct: ORF TRCN0000489751

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019333.1_s317c1
DNA Barcode:
GACCACGGCGGCAACATTCCAGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHRM3 (1131)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489751
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1131 CHRM3 cholinergic receptor muscar... NM_000740.3 99.9% 99.8% 1770_1771insG
2 human 1131 CHRM3 cholinergic receptor muscar... NM_001347716.2 99.9% 99.8% 1770_1771insG
3 human 1131 CHRM3 cholinergic receptor muscar... XM_005273032.3 99.9% 99.8% 1770_1771insG
4 human 1131 CHRM3 cholinergic receptor muscar... XM_011544041.2 99.9% 99.8% 1770_1771insG
5 human 1131 CHRM3 cholinergic receptor muscar... XM_011544043.2 99.9% 99.8% 1770_1771insG
6 human 1131 CHRM3 cholinergic receptor muscar... XM_011544044.2 99.9% 99.8% 1770_1771insG
7 human 1131 CHRM3 cholinergic receptor muscar... XM_011544046.2 99.9% 99.8% 1770_1771insG
8 human 1131 CHRM3 cholinergic receptor muscar... XM_011544047.2 99.9% 99.8% 1770_1771insG
9 human 1131 CHRM3 cholinergic receptor muscar... XM_017000152.2 99.9% 99.8% 1770_1771insG
10 human 1131 CHRM3 cholinergic receptor muscar... XM_017000153.1 99.9% 99.8% 1770_1771insG
11 human 1131 CHRM3 cholinergic receptor muscar... XM_017000154.1 99.9% 99.8% 1770_1771insG
12 human 1131 CHRM3 cholinergic receptor muscar... XM_017000155.1 99.9% 99.8% 1770_1771insG
13 human 1131 CHRM3 cholinergic receptor muscar... XM_017000156.1 99.9% 99.8% 1770_1771insG
14 human 1131 CHRM3 cholinergic receptor muscar... XM_017000157.2 99.9% 99.8% 1770_1771insG
15 human 1131 CHRM3 cholinergic receptor muscar... XM_017000158.1 99.9% 99.8% 1770_1771insG
16 human 1131 CHRM3 cholinergic receptor muscar... XM_017000159.1 99.9% 99.8% 1770_1771insG
17 human 1131 CHRM3 cholinergic receptor muscar... XM_017000160.2 99.9% 99.8% 1770_1771insG
18 human 1131 CHRM3 cholinergic receptor muscar... XM_017000161.1 99.9% 99.8% 1770_1771insG
19 human 1131 CHRM3 cholinergic receptor muscar... XM_017000162.1 99.9% 99.8% 1770_1771insG
20 human 1131 CHRM3 cholinergic receptor muscar... XM_017000163.2 99.9% 99.8% 1770_1771insG
21 mouse 12671 Chrm3 cholinergic receptor, musca... NM_033269.4 86.9% 91.5% (many diffs)
22 mouse 12671 Chrm3 cholinergic receptor, musca... XM_006516468.3 86.9% 91.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1845
ORF length:
1773
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaccttg cacaataaca gtacaacctc gcctttgttt ccaaacatca
121gctcctcctg gatacacagc ccctccgatg cagggctgcc cccgggaacc gtcactcatt
181tcggcagcta caatgtttct cgagcagctg gcaatttctc ctctccagac ggtaccaccg
241atgaccctct gggaggtcat accgtctggc aagtggtctt catcgctttc ttaacgggca
301tcctggcctt ggtgaccatc atcggcaaca tcctggtaat tgtgtcattt aaggtcaaca
361agcagctgaa gacggtcaac aactacttcc tcttaagcct ggcctgtgcc gatctgatta
421tcggggtcat ttcaatgaat ctgtttacga cctacatcat catgaatcga tgggccttag
481ggaacttggc ctgtgacctc tggcttgcca ttgactacgt agccagcaat gcctctgtta
541tgaatcttct ggtcatcagc tttgacagat acttttccat cacgaggccg ctcacgtacc
601gagccaaacg aacaacaaag agagccggtg tgatgatcgg tctggcttgg gtcatctcct
661ttgtcctttg ggctcctgcc atcttgttct ggcaatactt tgttggaaag agaactgtgc
721ctccgggaga gtgcttcatt cagttcctca gtgagcccac cattactttt ggcacagcca
781tcgctgcttt ttatatgcct gtcaccatta tgactatttt atactggagg atctataagg
841aaactgaaaa gcgtaccaaa gagcttgctg gcctgcaagc ctctgggaca gaggcagaga
901cagaaaactt tgtccacccc acgggcagtt ctcgaagctg cagcagttac gaacttcaac
961agcaaagcat gaaacgctcc aacaggagga agtatggccg ctgccacttc tggttcacaa
1021ccaagagctg gaaacccagc tccgagcaga tggaccaaga ccacagcagc agtgacagtt
1081ggaacaacaa tgatgctgct gcctccctgg agaactccgc ctcctccgac gaggaggaca
1141ttggctccga gacgagagcc atctactcca tcgtgctcaa gcttccgggt cacagcacca
1201tcctcaactc caccaagtta ccctcatcgg acaacctgca ggtgcctgag gaggagctgg
1261ggatggtgga cttggagagg aaagccgaca agctgcaggc ccagaagagc gtggacgatg
1321gaggcagttt tccaaaaagc ttctccaagc ttcccatcca gctagagtca gccgtggaca
1381cagctaagac ttctgacgtc aactcctcag tgggtaagag cacggccact ctacctctgt
1441ccttCAAGGA AGCCACTCTG GCCAAGAGGT TTGCTCTGAA GACCAGAAGT CAGATCACTA
1501AGCGGAAAAG GATGTCCCTG GTCAAGGAGA AGAAAGCGGC CCAGACCCTC AGTGCGATCT
1561TGCTTGCCTT CATCATCACT TGGACCCCAT ACAACATCAT GGTTCTGGTG AACACCTTTT
1621GTGACAGCTG CATACCCAAA ACCTTTTGGA ATCTGGGCTA CTGGCTGTGC TACATCAACA
1681GCACCGTGAA CCCCGTGTGC TATGCTCTGT GCAACAAAAC ATTCAGAACC ACTTTCAAGA
1741TGCTGCTGCT GTGCCAGTGT GACAAAAAAA AGAGGCGCAA GCAGCAGTAC CAGCAGAGAC
1801AGTCGGTCAT TTTTCACAAG CGCGCACCCG AGCAGGCCTT GGACCCAGCT TTCTTGTACA
1861AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1921AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1981AGGACGAGAC CACGGCGGCA ACATTCCAGC AACGCGTTAA GTCgacaatc aacctctgga
2041ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)