Construct: ORF TRCN0000489756
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020679.1_s317c1
- DNA Barcode:
- ACGCGTAAGGTGTACTAGAAAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TRIM24 (8805)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489756
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8805 | TRIM24 | tripartite motif containing 24 | NM_015905.3 | 99.9% | 100% | 1161G>A |
| 2 | human | 8805 | TRIM24 | tripartite motif containing 24 | NM_003852.4 | 96.7% | 96.7% | 1161G>A;1425_1426ins102 |
| 3 | human | 8805 | TRIM24 | tripartite motif containing 24 | XM_024446981.1 | 92.4% | 87.9% | (many diffs) |
| 4 | mouse | 21848 | Trim24 | tripartite motif-containing 24 | NM_145076.4 | 88.9% | 93.2% | (many diffs) |
| 5 | mouse | 21848 | Trim24 | tripartite motif-containing 24 | NM_001272064.1 | 85.7% | 90% | (many diffs) |
| 6 | mouse | 21848 | Trim24 | tripartite motif-containing 24 | NM_001272076.1 | 81.4% | 83.9% | (many diffs) |
| 7 | mouse | 21848 | Trim24 | tripartite motif-containing 24 | XM_006505895.2 | 65.8% | 68.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3222
- ORF length:
- 3150
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggtg gcggtggaga aggcggtggc ggcggcggca gcggcctcgg 121 ctgcggcctc cggggggccc tcggcggcgc cgagcgggga gaacgaggcc gagagtcggc 181 agggcccgga ctcggagcgc ggcggcgagg cggcccggct caacctgttg gacacttgcg 241 ccgtgtgcca ccagaacatc cagagccggg cgcccaagct gctgccctgc ctgcactctt 301 tctgccagcg ctgcctgccc gcgccccagc gctacctcat gctgcccgcg cccatgctgg 361 gctcggccga gaccccgcca cccgtccctg cccccggctc gccggtcagc ggctcgtcgc 421 cgttcgccac ccaagttgga gtcattcgtt gcccagtttg cagccaagaa tgtgcagaga 481 gacacatcat agataacttt tttgtgaagg acactactga ggttcccagc agtacagtag 541 aaaagtcaaa tcaggtatgt acaagctgtg aggacaacgc agaagccaat gggttttgtg 601 tagagtgtgt tgaatggctc tgcaagacgt gtatcagagc tcatcagagg gtaaagttca 661 caaaagacca cactgtcaga cagaaagagg aagtatctcc agaggcagtt ggtgtcacca 721 gccagcgacc agtgttttgt ccttttcata aaaaggagca gctgaagctg tactgtgaga 781 catgtgacaa actgacatgt cgagactgtc agttgttaga acataaagag catagatacc 841 aatttataga agaagctttt cagaatcaga aagtgatcat agatacacta atcaccaaac 901 tgatggaaaa aacaaaatac ataaaattca caggaaatca gatccaaaac agaattattg 961 aagtaaatca aaatcaaaag caggtggaac aggatattaa agttgctata tttacactga 1021 tggtagaaat aaataaaaaa ggaaaagctc tactgcatca gttagagagc cttgcaaagg 1081 accatcgcat gaaacttatg caacaacaac aggaagtggc tggactctct aaacaattgg 1141 agcatgtcat gcatttttct aaatgggcag tttccagtgg cagcagtaca gcattacttt 1201 atagcaaacg actgattaca taccggttac gacacctcct tcgtgcaagg tgtgatgcat 1261 ccccagtgac caacaacacc atccaatttc actgtgatcc tagtttctgg gctcaaaata 1321 tcatcaactt aggttcttta gtaatcgagg ataaagagag ccagccacaa atgcctaagc 1381 agaatcctgt cgtggaacag aattcacagc caccaagtgg tttatcatca aaccagttat 1441 ccaagttccc aacacagatc agcctagctc aattacggct ccagcatatg cagcaacagg 1501 taatggctca gaggcaacag gtgcaacgga ggccagcacc tgtgggttta ccaaacccta 1561 gaatgcaggg gcccatccag caaccttcca tctctcatca gcaaccgcct ccacgtttga 1621 taaactttca gaatcacagc cccaaaccca atggaccagt tcttcctcct catcctcaac 1681 aactgagata tccaccaaac cagaacatac cacgacaagc aataaagcca aaccccctac 1741 agatggcttt cttggctcaa caagccataa aacagtggca gatcagcagt ggacagggaa 1801 ccccatcaac taccaacagc acatcctcta ctccttccag ccccacgatt actagtgcag 1861 caggatatga tggaaaggct tttggttcac ctatgatcga tttgagctca ccagtgggag 1921 ggtcttataa tcttccctct cttccggata ttgactgttc aagtactatt atgctggaca 1981 atattgtgag gaaagatact aatatagatc atggccagcc aagaccaccc tcaaacagaa 2041 cggtccagtc accaaattca tcagtgccat ctccaggcct tgcaggacct gttactatga 2101 ctagtgtaca ccccccaata cgttcaccta gtgcctccag cgttggaagc cgaggaagct 2161 ctggctcttc cagcaaacca gcaggagctg actctacaca caaagtccca gtggtcatgc 2221 tggagccaat tcgaataaaa caagaaaaca gtggaccacc ggaaaattat gatttccctg 2281 ttgttatagt gaagcaagaa tcagatgaag aatctaggcc tcaaaatgcc aattatccaa 2341 gaagcatact cacctccctg ctcttaaata gcagtcagag ctctacttct gaggagactg 2401 tgctaagatc agatgcccct gatagtacag gagatcaacc tggacttcac caggacaatt 2461 cctcaaatgg aaagtctgaa tggttggatc cttcccagaa gtcacctctt catgttggag 2521 agacaaggaa agaggatgac cccaatgagg actggtgtgc agtttgtcaa aacggagggg 2581 aactcctctg ctgtgaaaag tgccccaaag tattccatct ttcttgtcat gtgcccacat 2641 tgacaaattt tccaagtgga gagtggattt gcactttctg ccgagactta tctaaaccag 2701 aagttgaata tgattgtgat gctcccagtc acaactcaga aaaaaagaaa actgaaggcc 2761 ttgttaagtt aacacctata gataaaagga agtgtgagcg cctactttta tttctttact 2821 gccatgaaat gagcctggct tttcaagacc ctgttcctct aactgtgccT GATTATTACA 2881 AAATAATTAA AAATCCAATG GATTTGTCAA CCATCAAGAA AAGACTACAA GAAGATTATT 2941 CCATGTACTC AAAACCTGAA GATTTTGTAG CTGATTTTAG ATTGATCTTT CAAAACTGTG 3001 CTGAATTCAA TGAGCCTGAT TCAGAAGTAG CCAATGCTGG TATAAAACTT GAAAATTATT 3061 TTGAAGAACT TCTAAAGAAC CTCTATCCAG AAAAAAGGTT TCCCAAACCA GAATTCAGGA 3121 ATGAATCAGA AGATAATAAA TTTAGTGATG ATTCAGATGA TGACTTTGTA CAGCCCCGGA 3181 AGAAACGCCT CAAAAGCATT GAAGAACGCC AGTTGCTTAA ATAGAACCCA GCTTTCTTGT 3241 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3301 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3361 GAAAGGACGA ACGCGTAAGG TGTACTAGAA AATCACGCGT TAAGTCgaca atcaacctct 3421 ggattacaaa atttgtgaaa gatt