Construct: ORF TRCN0000489764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019954.1_s317c1
- DNA Barcode:
- CTGGAGACAAGTGCGACAGCCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VDR (7421)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489764
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7421 | VDR | vitamin D receptor | NM_000376.3 | 99.9% | 99.7% | 1281_1282insG |
| 2 | human | 7421 | VDR | vitamin D receptor | NM_001017535.1 | 99.9% | 99.7% | 1281_1282insG |
| 3 | human | 7421 | VDR | vitamin D receptor | XM_006719587.3 | 99.9% | 99.7% | 1281_1282insG |
| 4 | human | 7421 | VDR | vitamin D receptor | XM_011538720.2 | 99.9% | 99.7% | 1281_1282insG |
| 5 | human | 7421 | VDR | vitamin D receptor | XM_024449178.1 | 94.8% | 94.6% | 1_69del;1350_1351insG |
| 6 | human | 7421 | VDR | vitamin D receptor | NM_001017536.2 | 89.4% | 89.3% | 1_150del;1431_1432insG |
| 7 | human | 7421 | VDR | vitamin D receptor | NM_001364085.1 | 86.4% | 99.7% | 1282_1482delinsG |
| 8 | mouse | 22337 | Vdr | vitamin D (1,25-dihydroxyvi... | NM_009504.4 | 86% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1356
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggca atggcggcca gcacttccct gcctgaccct ggagactttg 121 accggaacgt gccccggatc tgtggggtgt gtggagaccg agccactggc tttcacttca 181 atgctatgac ctgtgaaggc tgcaaaggct tcttcaggcg aagcatgaag cggaaggcac 241 tattcacctg ccccttcaac ggggactgcc gcatcaccaa ggacaaccga cgccactgcc 301 aggcctgccg gctcaaacgc tgtgtggaca tcggcatgat gaaggagttc attctgacag 361 atgaggaagt gcagaggaag cgggagatga tcctgaagcg gaaggaggag gaggccttga 421 aggacagtct gcggcccaag ctgtctgagg agcagcagcg catcattgcc atactgctgg 481 acgcccacca taagacctac gaccccacct actccgactt ctgccagttc cggcctccag 541 ttcgtgtgaa tgatggtgga gggagccatc cttccaggcc caactccaga cacactccca 601 gcttctctgg ggactcctcc tcctcctgct cagatcactg tatcacctct tcagacatga 661 tggactcgtc cagcttctcc aatctggatc tgagtgaaga agattcagat gacccttctg 721 tgaccctaga gctgtcccag ctctccatgc tgccccacct ggctgacctg gtcagttaca 781 gcatccaaaa ggtcattggc tttgctaaga tgataccagg attcagagac ctcacctctg 841 aggaccagat cgtactgctg aagtcaagtg ccattgaggt catcatgttg cgctccaatg 901 agtccttcac catggacgac atgtcctgga cctgtggcaa ccaagactac aagtaccgcg 961 tcagtgacgt gaccaaagcc ggacacagcc tggagctgat tgagccccTC ATCAAGTTCC 1021 AGGTGGGACT GAAGAAGCTG AACTTGCATG AGGAGGAGCA TGTCCTGCTC ATGGCCATCT 1081 GCATCGTCTC CCCAGATCGT CCTGGGGTGC AGGACGCCGC GCTGATTGAG GCCATCCAGG 1141 ACCGCCTGTC CAACACACTG CAGACGTACA TCCGCTGCCG CCACCCGCCC CCGGGCAGCC 1201 ACCTGCTCTA TGCCAAGATG ATCCAGAAGC TAGCCGACCT GCGCAGCCTC AATGAGGAGC 1261 ACTCCAAGCA GTACCGCTGC CTCTCCTTCC AGCCTGAGTG CAGCATGAAG CTAACGCCCC 1321 TTGTGCTCGA AGTGTTTGGC AATGAGATCT CCGACCCAGC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 1501 GGAGACAAGT GCGACAGCCA TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt