Construct: ORF TRCN0000489770
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021894.1_s317c1
- DNA Barcode:
- GAAGAAAACACCGAGAGAACTGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HDAC6 (10013)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489770
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321226.2 | 99.9% | 100% | 2265A>G |
| 2 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321227.2 | 99.9% | 100% | 2265A>G |
| 3 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321228.2 | 99.9% | 100% | 2265A>G |
| 4 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321229.1 | 99.9% | 100% | 2265A>G |
| 5 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_006044.4 | 99.9% | 100% | 2265A>G |
| 6 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321225.2 | 98.8% | 98.8% | 1_42del;2307A>G |
| 7 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_002958756.1 | 85.7% | (many diffs) | |
| 8 | human | 10013 | HDAC6 | histone deacetylase 6 | NR_135591.1 | 85.2% | (many diffs) | |
| 9 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_246712.2 | 84.3% | (many diffs) | |
| 10 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_001755632.2 | 83.8% | (many diffs) | |
| 11 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_002958757.1 | 81% | (many diffs) | |
| 12 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_002958755.1 | 80.7% | 1_79del;2071_2072ins343;3382_3748del | |
| 13 | human | 10013 | HDAC6 | histone deacetylase 6 | XM_017029187.1 | 49.4% | 48.7% | 1_42del;1832_1833ins134;1866_1867ins1687 |
| 14 | human | 10013 | HDAC6 | histone deacetylase 6 | NR_135592.2 | 27.2% | (many diffs) | |
| 15 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321230.2 | 11.9% | 11.9% | 438_438delGins3208 |
| 16 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321231.2 | 11.9% | 11.9% | 438_438delGins3208 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3717
- ORF length:
- 3645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacctca accggccagg attccaccac aaccaggcag cgaagaagta 121 ggcagaaccc ccagtcgccc cctcaggact ccagtgtcac ttcgaagcga aatattaaaa 181 agggagccgt tccccgctct atccccaatc tagcggaggt aaagaagaaa ggcaaaatga 241 agaagctcgg ccaagcaatg gaagaagacc taatcgtggg actgcaaggg atggatctga 301 accttgaggc tgaagcactg gctggcactg gcttggtgtt ggatgagcag ttaaatgaat 361 tccattgcct ctgggatgac agcttcccgg aaggccctga gcggctccat gccatcaagg 421 agcaactgat ccaggagggc ctcctagatc gctgcgtgtc ctttcaggcc cggtttgctg 481 aaaaggaaga gctgatgttg gttcacagcc tagaatatat tgatctgatg gaaacaaccc 541 agtacatgaa tgagggagaa ctccgtgtcc tagcagacac ctacgactca gtttatctgc 601 atccgaactc atactcctgt gcctgcctgg cctcaggctc tgtcctcagg ctggtggatg 661 cggtcctggg ggctgagatc cggaatggca tggccatcat taggcctcct ggacatcacg 721 cccagcacag tcttatggat ggctattgca tgttcaacca cgtggctgtg gcagcccgct 781 atgctcaaca gaaacaccgc atccggaggg tccttatcgt agattgggat gtgcaccacg 841 gtcaaggaac acagttcacc ttcgaccagg accccagtgt cctctatttc tccatccacc 901 gctacgagca gggtaggttc tggccccacc tgaaggcctc taactggtcc accacaggtt 961 tcggccaagg ccaaggatat accatcaatg tgccttggaa ccaggtgggg atgcgggatg 1021 ctgactacat tgctgctttc ctgcacgtcc tgctgccagt cgccctcgag ttccagcctc 1081 agctggtcct ggtggctgct ggatttgatg ccctgcaagg ggaccccaag ggtgagatgg 1141 ccgccactcc ggcagggttc gcccagctaa cccacctgct catgggtctg gcaggaggca 1201 agctgatcct gtctctggag ggtggctaca acctccgcgc cctggctgaa ggcgtcagtg 1261 cttcgctcca cacccttctg ggagaccctt gccccatgct ggagtcacct ggtgccccct 1321 gccggagtgc ccaggcttca gtttcctgtg ctctggaagc ccttgagccc ttctgggagg 1381 ttcttgtgag atcaactgag accgtggaga gggacaacat ggaggaggac aatgtagagg 1441 agagcgagga ggaaggaccc tgggagcccc ctgtgctccc aatcctgaca tggccagtgc 1501 tacagtctcg cacagggctg gtctatgacc aaaatatgat gaatcactgc aacttgtggg 1561 acagccacca ccctgaggta ccccagcgca tcttgcggat catgtgccgt ctggaggagc 1621 tgggccttgc cgggcgctgc ctcaccctga caccgcgccc tgccacagag gctgagctgc 1681 tcacctgtca cagtgctgag tacgtgggtc atctccgggc cacagagaaa atgaaaaccc 1741 gggagctgca ccgtgagagt tccaactttg actccatcta tatctgcccc agtaccttcg 1801 cctgtgcaca gcttgccact ggcgctgcct gccgcctggt ggaggctgtg ctctcaggag 1861 aggttctgaa tggtgctgct gtggtgcgtc ccccaggaca ccacgcagag caggatgcag 1921 cttgcggttt ttgctttttc aactctgtgg ctgtggctgc tcgccatgcc cagactatca 1981 gtgggcatgc cctacggatc ctgattgtgg attgggatgt ccaccacggt aatggaactc 2041 agcacatgtt tgaggatgac cccagtgtgc tatatgtgtc cctgcaccgc tatgatcatg 2101 gcaccttctt ccccatgggg gatgagggtg ccagcagcca gatcggccgg gctgcgggca 2161 caggcttcac cgtcaacgtg gcatggaacg ggccccgcat gggtgatgct gactacctag 2221 ctgcctggca tcgcctggtg cttcccattg cctacgagtt taacccagaa ctggtgctgg 2281 tctcagctgg ctttgatgct gcacgggggg atccgctggg gggctgccag gtgtcgcctg 2341 agggttatgc ccacctcacc cacctgctga tgggccttgc cagtggccgc attatcctta 2401 tcctagaggg tggctataac ctgacatcca tctcagagtc catggctgcc tgcactcgct 2461 ccctccttgg agacccacca cccctgctga ccctgccacg gcccccacta tcaggggccc 2521 tggcctcaat cactgagacc atccaagtcc atcgcagata ctggcgcagc ttacgggtca 2581 tgaaggtaga agacagagaa ggaccctcca gttctaagtt ggtcaccaag aaggcacccc 2641 aaccagccaa acctaggtta gctgagcgga tgaccacacg agaaaagaag gttctggaag 2701 caggcatggg gaaagtcacc tcggcatcat ttggggaaga gtccactcca ggccagacta 2761 actcagagac agctgtggtg gccctcactc aggaccagcc ctcagaggca gccacagggg 2821 gagccactct ggcccagacc atttctgagg cagccattgg gggagccatg ctgggccaga 2881 ccacctcaga ggaggctgtc gggggagcca ctccggacca gaccacctca gaggagactg 2941 tgggaggagc cattctggac cagaccacct cagaggatgc tgttggggga gccacgctgg 3001 gccagactac ctcagaggag gctgtaggag gagctacact ggcccagacc acctcggagg 3061 cagccatgga gggagccaca ctggaccaga ctacgtcaga ggaggctcca gggggcaccg 3121 agctgatcca aactcctcta gcctcgagca cagaccacca gaccccccca acctcacctg 3181 tgcagggaac tacaccccag atatctccca gtacactgat tgggagtctc aggaccttgg 3241 agctaggcag cgaatctcag ggggcctcag aatctcaggc cccaggagag gagaacctac 3301 taggagaggc agctggaggt caggacatgg ctgattcgat gctgatgcag ggatctaggg 3361 gcctcactga tcaggccata tTTTATGCTG TGACACCACT GCCCTGGTGT CCCCATTTGG 3421 TGGCAGTATG CCCCATACCT GCAGCAGGCC TAGACGTGAC CCAACCTTGT GGGGACTGTG 3481 GAACAATCCA AGAGAATTGG GTGTGTCTCT CTTGCTATCA GGTCTACTGT GGTCGTTACA 3541 TCAATGGCCA CATGCTCCAA CACCATGGAA ATTCTGGACA CCCGCTGGTC CTCAGCTACA 3601 TCGACCTGTC AGCCTGGTGT TACTACTGTC AGGCCTATGT CCACCACCAG GCTCTCCTAG 3661 ATGTGAAGAA CATCGCCCAC CAGAACAAGT TTGGGGAGGA TATGCCCCAC CCACACTAAG 3721 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3841 TTTATATATC TTGTGGAAAG GACGAGAAGA AAACACCGAG AGAACTGCAA CGCGTTAAGT 3901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt