Construct: ORF TRCN0000489777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020916.1_s317c1
- DNA Barcode:
- TGACATACCTCCTTCTTAAACTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRB3 (577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489777
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 577 | ADGRB3 | adhesion G protein-coupled ... | NM_001704.3 | 99.9% | 99.9% | 1508A>G;4008G>A |
| 2 | mouse | 210933 | Adgrb3 | adhesion G protein-coupled ... | NM_175642.4 | 92.3% | 98.4% | (many diffs) |
| 3 | mouse | 210933 | Adgrb3 | adhesion G protein-coupled ... | XM_006495817.4 | 90.2% | 96.1% | (many diffs) |
| 4 | mouse | 210933 | Adgrb3 | adhesion G protein-coupled ... | XM_017319555.2 | 71.6% | 76.3% | (many diffs) |
| 5 | mouse | 210933 | Adgrb3 | adhesion G protein-coupled ... | XM_006495818.4 | 56.5% | 60.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4638
- ORF length:
- 4566
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaggct gttcgtaacc tgctgattta tatattttcc acctatctcc 121 tggttatgtt tggatttaat gctgcccaag acttctggtg ttcaactttg gtgaagggag 181 tcatttatgg atcgtattct gtaagtgaaa tgtttcctaa aaactttaca aactgcactt 241 ggacgctgga aaatccagat ccaaccaaat atagcattta cctgaaattt tccaaaaagg 301 accttagctg ctctaacttt tcactcctgg cttatcagtt tgatcatttt tcccatgaaa 361 aaataaagga tcttttaaga aagaatcatt ctataatgca actctgcaat tccaagaatg 421 ctttcgtttt tctacagtat gataaaaatt ttattcaaat acgtcgagta tttccaacta 481 atttcccagg attacagaaa aaaggggaag aagatcagaa atcttttttt gagtttttgg 541 tattgaacaa ggtcagccca agccagtttg gttgccatgt attatgtact tggttggaga 601 gctgcttaaa atcagaaaat gggagaacag aatcatgtgg gatcatgtat acaaaatgca 661 cctgccctca gcatttggga gagtggggga tcgacgacca gtcgctgatt ttgttaaata 721 acgtggtgtt acccctgaat gagcagacag agggctgcct gacccaggag ctgcaaacca 781 cccaagtctg caatcttacc agggaggcca agcgaccacc caaagaagaa tttggaatga 841 tgggagatca tacaattaaa agtcagcgac ctcgatctgt tcatgaaaaa agggtccctc 901 aggaacaagc tgatgctgct aaatttatgg cacaaactgg tgaatctggt gtggaagagt 961 ggtcccagtg gagcacatgt tcggttactt gtggtcaagg gtcgcaggtg cgaaccagaa 1021 cttgtgtatc accttacggg acacactgca gcggcccatt aagagaatca agggtttgca 1081 ataacactgc cctctgtcca gtacacggag tatgggagga atggtcacca tggagtttat 1141 gttcatttac atgtggtcga ggccaaagaa caagaacaag gtcatgcaca cctcctcagt 1201 atggaggaag gccgtgtgaa ggacctgaaa cacatcataa gccttgtaat attgctcttt 1261 gcccagttga tggacagtgg caagagtgga gttcgtggag ccagtgctca gtaacgtgct 1321 cgaatgggac tcagcagaga agccggcagt gcactgcagc tgcccatgga ggctccgaat 1381 gcagagggcc atgggcagaa agcagagagt gctataaccc tgaatgtaca gccaatggtc 1441 aatggaatca gtggggtcat tggagtggtt gttccaagtc ctgtgatggc ggctgggaaa 1501 ggcgaataag gacctgtcag ggtgcagtga taacagggca gcaatgtgaa ggaacgggcg 1561 aagaagtgag aagatgcagt gagcagcgat gccctgcacc ttatgaaata tgccctgagg 1621 attatctgat gtcgatggtg tggaaaagaa ctccagcagg cgacttggca ttcaatcaat 1681 gtcccctgaa tgccacaggc accactagca gacgctgctc tctcagtctt catggagtgg 1741 ccttctggga acagccgagc tttgcaagat gcatatcaaa tgagtacaga cacttgcagc 1801 attcaattaa agagcacctt gctaaggggc agcgaatgct ggcaggtgat ggaatgtccc 1861 aggtgaccaa gacactgttg gatttaactc agagaaaaaa tttctatgca ggcgatcttc 1921 tgatgtctgt ggagatcctg agaaatgtga cagacacatt taaaagggca agttacatcc 1981 ctgcatctga tggtgtccag aacttctttc aaatagttag caaccttcta gatgaagaaa 2041 acaaggaaaa atgggaagat gcacaacaga tttatccagg gtcaatagag ttaatgcagg 2101 tgattgaaga ttttatacac attgttggaa tggggatgat ggactttcag aattcatact 2161 taatgactgg aaatgtagtg gctagtattc agaagcttcc tgcagcctct gttctaacag 2221 acatcaactt tccaatgaaa ggacggaagg gaatggttga ctgggcaaga aactcagaag 2281 atagggtagt aattccaaaa agcattttca ctccggtgtc atcaaaagaa ttagatgaat 2341 catctgtatt tgttcttggc gcagtcctat acaaaaactt agatctaatt ttgcccactt 2401 tgagaaatta tactgtcatt aattccaaaa tcatcgtggt cacaataagg cctgaaccca 2461 aaacaaccga ttcgtttctg gagatagaac tagctcattt ggctaatggt actttgaatc 2521 cctattgtgt attgtgggat gactccaaaa cgaacgagtc tttgggaacg tggtccaccc 2581 agggatgtaa aactgtgctt accgatgcat cccatacgaa atgcttatgt gatcgtctct 2641 ctaccttcgc cattttggct cagcaaccta gagaaataat catggaatcc tctggcacac 2701 cttcagttac cctaatagta ggcagtggtc tttcttgctt ggccttgatt accctagcag 2761 ttgtctatgc agcattatgg aggtacatac gctctgagag atccataata ctaattaact 2821 tctgcctgtc tatcatctca tccaatatcc tcatactggt tggacagact cagacacata 2881 ataagagtat ctgcacaacc accactgcat ttttgcactt tttcttcctg gcttcattct 2941 gttgggtttt gactgaggcg tggcaatcat atatggctgt aactggaaaa attaggacac 3001 ggcttataag aaaacgcttt ttgtgccttg gatggggttt accagcatta gtagtggcca 3061 catcagtagg cttcaccaga acaaaaggat atggcactga tcactactgc tggctctctc 3121 ttgaaggagg actactctat gcttttgtgg gacctgcagc cgctgttgtc ctggtcaaca 3181 tggtgattgg cattttggta tttaataaac ttgtttccag agatggaatc ctagataaaa 3241 agctcaaaca cagagccggt cagatgagtg agcctcatag cggtttgacg ctcaaatgtg 3301 ccaagtgtgg agtagtttca acaacagctt tgtcagccac caccgccagt aacgccatgg 3361 cgtctctttg gagctcctgt gtggtgttgc cccttctggc tttgacgtgg atgtctgcgg 3421 ttctggccat gacagataaa cgctccatat tgtttcaaat actttttgct gtgtttgatt 3481 cattgcaagg ctttgttata gtcatggtcc actgcattct tcggagagag gttcaggatg 3541 catttagatg ccgattgaga aactgtcagg atcccatcaa tgcagattct tcgagttcgt 3601 ttcctaatgg gcatgctcaa atcatgacag actttgaaaa ggatgtagac attgcctgtc 3661 gatcagttct tcataaggat attggtcctt gccgagcagc cacaataaca ggaacacttt 3721 ctaggatttc tctaaatgat gatgaagaag aaaagggaac aaaccctgaa gggctaagct 3781 attcaacatt gcctggaaat gtcatttcca aagtcatcat ccagcaaccc acaggtttgc 3841 acatgcccat gagtatgaat gagcttagca atccatgttt gaaaaaagaa aatagtgaat 3901 tgcggagaac tgtgtactta tgtacggatg ataatttgag aggggctgac atggacatag 3961 tccatcctca agaaagaatg atggaaagtg actatattgt gatgcccaga agttctgtaa 4021 ataaccagcc ttcaatgaaa gaagaaagca aaatgaatat tggcatggaa accttgccac 4081 atgaaaggct attgcactac aaagtaaacc ctgaattcaa tatgaatccc cctgtaatgg 4141 accagttcaa tatgaactta gagcaacatc tcgcacccca ggaacatatg cagaatttgc 4201 cctttgaacc tcgcacagct gtgaagaatt tcatggcctc tgagttggat gataatgcag 4261 gactatcaag aagtgaaact ggaTCAACGA TATCAATGAG TTCTTTAGAG AGAAGAAAAT 4321 CACGATATTC AGACCTTGAC TTTGAGAAGG TCATGCATAC AAGGAAGAGG CATATGGAAC 4381 TATTTCAAGA ACTAAATCAG AAATTTCAAA CTTTGGACAG ATTTCGGGAT ATACCAAATA 4441 CAAGCAGTAT GGAAAACCCC GCACCAAACA AGAATCCATG GGACACTTTC AAAAACCCCA 4501 GTGAATACCC GCATTACACC ACAATCAATG TCTTAGACAC AGAGGCAAAG GATGCTTTGG 4561 AACTGAGGCC AGCAGAGTGG GAGAAGTGTC TGAATTTGCC TCTGGATGTG CAAGAGGGTG 4621 ACTTTCAAAC AGAAGTTTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 4681 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 4741 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGAC ATACCTCCTT 4801 CTTAAACTTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt