Construct: ORF TRCN0000489789
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019644.1_s317c1
- DNA Barcode:
- AGTCACTTGGGAACTCGTTTGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNG7 (2788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489789
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2788 | GNG7 | G protein subunit gamma 7 | NM_052847.3 | 99.5% | 98.5% | 204_205insG |
| 2 | human | 2788 | GNG7 | G protein subunit gamma 7 | XM_017026606.1 | 99.5% | 98.5% | 204_205insG |
| 3 | mouse | 14708 | Gng7 | guanine nucleotide binding ... | NM_001038655.1 | 81.7% | 91.4% | (many diffs) |
| 4 | mouse | 14708 | Gng7 | guanine nucleotide binding ... | NM_010319.3 | 81.7% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 240
- ORF end:
- 447
- ORF length:
- 207
- Sequence:
-
1 GTCTTTAGGT TGGGGGGAGG GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA 61 GACTGAAGTT AGGCCAGCTT GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA 121 GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT 181 TCAGGTGTCG TGAGGCTAGC ATCGATTGAT CAACAAGTTT GTACAAAAAA GCAGGCTTCA 241 TGTCAGCCAC TAACAACATA GCCCAGGCCC GGAAGCTGGT GGAACAGCTA CGCATAGAAG 301 CCGGGATTGA GCGCATCAAG GTCTCCAAAG CGGCGTCTGA CCTCATGAGC TACTGTGAGC 361 AACATGCCCG GAACGACCCC CTGCTGGTCG GAGTCCCTGC CTCGGAGAAC CCCTTTAAGG 421 ACAAGAAACC TTGTATTATT TTAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GTCACTTGGG 601 AACTCGTTTG ACTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att