Construct: ORF TRCN0000489792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019498.1_s317c1
- DNA Barcode:
- GGCTGGCGCCTCCGGCAGGACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIMK2 (3985)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3985 | LIMK2 | LIM domain kinase 2 | NM_005569.4 | 99.9% | 100% | 1209C>G |
| 2 | human | 3985 | LIMK2 | LIM domain kinase 2 | NM_016733.3 | 95.3% | 94.5% | (many diffs) |
| 3 | human | 3985 | LIMK2 | LIM domain kinase 2 | NM_001031801.2 | 83.9% | 74.3% | (many diffs) |
| 4 | mouse | 16886 | Limk2 | LIM motif-containing protei... | NM_010718.4 | 88.2% | 93.1% | (many diffs) |
| 5 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_006514548.2 | 87.4% | 92.2% | (many diffs) |
| 6 | mouse | 16886 | Limk2 | LIM motif-containing protei... | NM_173053.1 | 85.6% | 89.9% | (many diffs) |
| 7 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_006514550.3 | 84.8% | 89.1% | (many diffs) |
| 8 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_006514547.1 | 80.5% | 84.9% | (many diffs) |
| 9 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_011243666.2 | 77.3% | 82.7% | (many diffs) |
| 10 | mouse | 16886 | Limk2 | LIM motif-containing protei... | NM_001034030.2 | 62.9% | 67.2% | (many diffs) |
| 11 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_006514552.3 | 62.3% | 66.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 2004
- ORF length:
- 1914
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccaag cttgggacca tgtccgcgct ggcgggtgaa gatgtctgga 121 ggtgtccagg ctgtggggac cacattgctc caagccagat atggtacagg actgtcaacg 181 aaacctggca cggctcttgc ttccggtgtt cagaatgcca ggattccctc accaactggt 241 actatgagaa ggatgggaag ctctactgcc ccaaggacta ctgggggaag tttggggagt 301 tctgtcatgg gtgctccctg ctgatgacag ggccttttat ggtggctggg gagttcaagt 361 accacccaga gtgctttgcc tgtatgagct gcaaggtgat cattgaggat ggggatgcat 421 atgcactggt gcagcatgcc accctctact gtgggaagtg ccacaatgag gtggtgctgg 481 cacccatgtt tgagagactc tccacagagt ctgttcagga gcagctgccc tactctgtca 541 cgctcatctc catgccggcc accactgaag gcaggcgggg cttctccgtg tccgtggaga 601 gtgcctgctc caactacgcc accactgtgc aagtgaaaga ggtcaaccgg atgcacatca 661 gtcccaacaa tcgaaacgcc atccaccctg gggaccgcat cctggagatc aatgggaccc 721 ccgtccgcac acttcgagtg gaggaggtgg aggatgcaat tagccagacg agccagacac 781 ttcagctgtt gattgaacat gaccccgtct cccaacgcct ggaccagctg cggctggagg 841 cccggctcgc tcctcacatg cagaatgccg gacaccccca cgccctcagc accctggaca 901 ccaaggagaa tctggagggg acactgagga gacgttccct aaggcgcagt aacagtatct 961 ccaagtcccc tggccccagc tccccaaagg agcccctgct gttcagccgt gacatcagcc 1021 gctcagaatc ccttcgttgt tccagcagct attcacagca gatcttccgg ccctgtgacc 1081 taatccatgg ggaggtcctg gggaagggct tctttgggca ggctatcaag gtgacacaca 1141 aagccacggg caaagtgatg gtcatgaaag agttaattcg atgtgatgag gagacccaga 1201 aaacttttct gactgaggtg aaagtgatgc gcagcctgga ccaccccaat gtgctcaagt 1261 tcattggtgt gctgtacaag gataagaagc tgaacctgct gacagagtac attgaggggg 1321 gcacactgaa ggactttctg cgcagtatgg atccgttccc ctggcagcag aaggtcaggt 1381 ttgccaaagg aatcgcctcc ggaatggcct atttgcactc tatgtgcatc atccaccggg 1441 atctgaactc gcacaactgc ctcatcaagt tggacaagac tgtggtggtg gcagactttg 1501 ggctgtcacg gctcatagtg gaagagagga aaagggcccc catggagaag gccaccacca 1561 agaaacgcac cttgcgcaag aacgaccgca agaagcgcta cacggtggtg ggaaacccct 1621 actggatggc ccctGAGATG CTGAACGGAA AGAGCTATGA TGAGACGGTG GATATCTTCT 1681 CCTTTGGGAT CGTTCTCTGT GAGATCATTG GGCAGGTGTA TGCAGATCCT GACTGCCTTC 1741 CCCGAACACT GGACTTTGGC CTCAACGTGA AGCTTTTCTG GGAGAAGTTT GTTCCCACAG 1801 ATTGTCCCCC GGCCTTCTTC CCGCTGGCCG CCATCTGCTG CAGACTGGAG CCTGAGAGCA 1861 GACCAGCATT CTCGAAATTG GAGGACTCCT TTGAGGCCCT CTCCCTGTAC CTGGGGGAGC 1921 TGGGCATCCC GCTGCCTGCA GAGCTGGAGG AGTTGGACCA CACTGTGAGC ATGCAGTACG 1981 GCCTGACCCG GGACTCACCT CCCAAGGGTG GGCGCGCCGA CCCAGCTTTC TTGTACAAAG 2041 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2101 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2161 ACGAGGCTGG CGCCTCCGGC AGGACCCCAC GCGTTAAGTC gacaatcaac ctctggatta 2221 caaaatttgt gaaagatt