Construct: ORF TRCN0000489793
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019417.1_s317c1
- DNA Barcode:
- CGTGGTTAGAGCTGTGTCAAACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR1F (3355)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489793
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | NM_000866.4 | 99.9% | 99.7% | 1098_1099insG |
| 2 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | NM_001322208.2 | 99.9% | 99.7% | 1098_1099insG |
| 3 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | NM_001322209.2 | 99.9% | 99.7% | 1098_1099insG |
| 4 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | NM_001322210.2 | 99.9% | 99.7% | 1098_1099insG |
| 5 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | XM_005264751.3 | 99.9% | 99.7% | 1098_1099insG |
| 6 | human | 3355 | HTR1F | 5-hydroxytryptamine recepto... | XM_011533664.2 | 99.9% | 99.7% | 1098_1099insG |
| 7 | mouse | 15557 | Htr1f | 5-hydroxytryptamine (seroto... | NM_008310.3 | 87.8% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1176
- ORF length:
- 1101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggat ttcttaaatt catctgatca aaacttgacc tcagaggaac 121 tgttaaacag aatgccatcc aaaattctgg tgtccctcac tctgtctggg ctggcactga 181 tgacaacaac tatcaactcc cttgtgatcg ctgcaattat tgtgacccgg aagctgcacc 241 atccagccaa ttatttaatt tgttcccttg cagtcacaga ttttcttgtg gctgtcctgg 301 tgatgccctt cagcattgtg tatattgtga gagagagctg gattatgggg caagtggtct 361 gtgacatttg gctgagtgtt gacattacct gctgcacgtg ctccatcttg catctctcag 421 ctatagcttt ggatcggtat cgagcaatca cagatgctgt tgagtatgcc aggaaaagga 481 ctccaaagca tgctggcatt atgattacaa tagtttggat tatatctgtt tttatctcta 541 tgcctcctct attctggagg caccaaggaa ctagcagaga tgatgaatgc atcatcaagc 601 acgaccacat tgtttccacc atttactcaa catttggagc tttctacatc ccactggcat 661 tgattttgat cctttactac aaaatatata gagcagcaaa gacattatac cacaagagac 721 aagcaagtag gattgcaaag gaggaggtga atggCCAAGT CCTTTTGGAG AGTGGTGAGA 781 AAAGCACTAA ATCAGTTTCC ACATCCTATG TACTAGAAAA GTCTTTATCT GACCCATCAA 841 CAGACTTTGA TAAAATTCAT AGCACAGTGA GAAGTCTCAG GTCTGAATTC AAGCATGAGA 901 AATCTTGGAG AAGGCAAAAG ATCTCAGGTA CAAGAGAACG GAAAGCAGCC ACTACCCTGG 961 GATTAATCTT GGGTGCATTT GTAATATGTT GGCTTCCTTT TTTTGTAAAA GAATTAGTTG 1021 TTAATGTCTG TGACAAATGT AAAATTTCTG AAGAAATGTC CAATTTTTTG GCATGGCTTG 1081 GGTATCTCAA TTCCCTTATA AATCCACTGA TTTACACAAT CTTTAATGAA GACTTCAAGA 1141 AAGCATTCCA AAAGCTTGTG CGATGTCGAT GTGACCCAGC TTTCTTGTAC AAAGTGGTTG 1201 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1261 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 1321 TGGTTAGAGC TGTGTCAAAC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1381 ttgtgaaaga tt