Construct: ORF TRCN0000489796
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021985.1_s317c1
- DNA Barcode:
- TCGCCCCTTGGAGTGATACTTGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PXDN (7837)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489796
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7837 | PXDN | peroxidasin | NM_012293.3 | 100% | 100% | |
| 2 | human | 7837 | PXDN | peroxidasin | XM_005264707.3 | 98.3% | 98.3% | 342_343ins72 |
| 3 | human | 7837 | PXDN | peroxidasin | XM_011510396.1 | 87.6% | 87.6% | 0_1ins549 |
| 4 | mouse | 69675 | Pxdn | peroxidasin | NM_181395.2 | 84% | 91% | (many diffs) |
| 5 | mouse | 69675 | Pxdn | peroxidasin | XM_006515205.3 | 79.6% | 86.3% | (many diffs) |
| 6 | mouse | 69675 | Pxdn | peroxidasin | XM_006515206.1 | 44.2% | 47.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4506
- ORF length:
- 4437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggccaagcgc tccaggggcc ccgggcgccg ctgcctgttg gcgctcgtgc 121 tgttctgcgc ctgggggacg ctggccgtgg tggcccagaa gccgggcgca gggtgtccga 181 gccgctgcct gtgcttccgc accaccgtgc gctgcatgca tctgctgctg gaggccgtgc 241 ccgccgtggc gccgcagacc tccatcctag atcttcgctt taacagaatc agagagatcc 301 aacctggggc attcaggcgg ctgaggaact tgaacacatt gcttctcaat aataatcaga 361 tcaagaggat acctagtgga gcatttgaag acttggaaaa tttaaaatat ctctatctgt 421 acaagaatga gatccagtca attgacaggc aagcatttaa gggacttgcc tctctagagc 481 aactatacct gcactttaat cagatagaaa ctttggaccc agattcgttc cagcatctcc 541 cgaagctcga gaggctattt ttgcataaca accggattac acatttagtt ccagggacat 601 ttaatcactt ggaatctatg aagagattgc gactggactc aaacacactt cactgcgact 661 gtgaaatcct gtggttggcg gatttgctga aaacctacgc ggagtcgggg aacgcgcagg 721 cagcggccat ctgtgaatat cccagacgca tccagggacg ctcagtggca accatcaccc 781 cggaagagct gaactgtgaa aggccccgga tcacctccga gccccaggac gcagatgtga 841 cctcggggaa caccgtgtac ttcacctgca gagccgaagg caaccccaag cctgagatca 901 tctggctgcg aaacaataat gagctgagca tgaagacaga ttcccgccta aacttgctgg 961 acgatgggac cctgatgatc cagaacacac aggagacaga ccagggtatc taccagtgca 1021 tggcaaagaa cgtggccgga gaggtgaaga cgcaagaggt gaccctcagg tacttcgggt 1081 ctccagctcg acccactttt gtaatccagc cacagaatac agaggtgctg gttggggaga 1141 gcgtcacgct ggagtgcagc gccacaggcc accccccgcc gcggatctcc tggacgagag 1201 gtgaccgcac acccttgcca gttgacccgc gggtgaacat cacgccttct ggcgggcttt 1261 acatacagaa cgtcgtacag ggggacagcg gagagtatgc gtgctctgcg accaacaaca 1321 ttgacagcgt ccatgccacc gctttcatca tcgtccaggc tcttcctcag ttcactgtga 1381 cgcctcagga cagagtcgtt attgagggcc agaccgtgga tttccagtgt gaagccaagg 1441 gcaacccgcc gcccgtcatc gcctggacca agggagggag ccagctctcc gtggaccggc 1501 ggcacctggt cctgtcatcg ggaacactta gaatctctgg tgttgccctc cacgaccagg 1561 gccagtacga atgccaggct gtcaacatca tcggctccca gaaggtcgtg gcccacctga 1621 ctgtgcagcc cagagtcacc ccagtgtttg ccagcattcc cagcgacaca acagtggagg 1681 tgggcgccaa tgtgcagctc ccgtgcagct cccagggcga gcccgagcca gccatcacct 1741 ggaacaagga tggggttcag gtgacagaaa gtggaaaatt tcacatcagc cctgaaggat 1801 tcttgaccat caatgacgtt ggccctgcag acgcaggtcg ctatgagtgt gtggcccgga 1861 acaccattgg gtcggcctcg gtgagcatgg tgctcagtgt gaatgttcct gacgtcagtc 1921 gaaatggaga tccgtttgta gctacctcca tcgtggaagc gattgcgact gttgacagag 1981 ctataaactc aacccgaaca catttgtttg acagccgtcc tcgttctcca aatgatttgc 2041 tggccttgtt ccggtatccg agggatcctt acacagttga acaggcacgg gcgggagaaa 2101 tctttgaacg gacattgcag ctcattcagg agcatgtaca gcatggcttg atggtcgacc 2161 tcaacggaac aagttaccac tacaacgacc tggtgtctcc acagtacctg aacctcatcg 2221 caaacctgtc gggctgtacc gcccaccggc gcgtgaacaa ctgctcggac atgtgcttcc 2281 accagaagta ccggacgcac gacggcacct gtaacaacct gcagcacccc atgtggggcg 2341 cctcgctgac cgccttcgag cgcctgctga aatccgtgta cgagaatggc ttcaacaccc 2401 ctcggggcat caacccccac cgactgtaca acgggcacgc ccttcccatg ccgcgcctgg 2461 tgtccaccac cctgatcggg acggagaccg tcacacccga cgagcagttc acccacatgc 2521 tgatgcagtg gggccagttc ctggaccacg acctcgactc cacggtggtg gccctgagcc 2581 aggcacgctt ctccgacgga cagcactgca gcaacgtgtg cagcaacgac cccccctgct 2641 tctctgtcat gatccccccc aatgactccc gggccaggag cggggcccgc tgcatgttct 2701 tcgtgcgctc cagccctgtg tgcggcagcg gcatgacttc gctgctcatg aactccgtgt 2761 acccgcggga gcagatcaac cagctcacct cctacataga cgcatccaac gtgtacggga 2821 gcacggagca tgaggcccgc agcatccgcg acctggccag ccaccgcggc ctgctgcggc 2881 agggcatcgt gcagcggtcc gggaagccgc tgctcccctt cgccaccggg ccgcccacgg 2941 agtgcatgcg ggacgagaac gagagcccca tcccctgctt cctggccggg gaccaccgcg 3001 ccaacgagca gctgggcctg accagcatgc acacgctgtg gttccgcgag cacaaccgca 3061 ttgccacgga gctgctcaag ctgaacccgc actgggacgg cgacaccatc tactatgaga 3121 ccaggaagat cgtgggtgcg gagatccagc acatcaccta ccagcactgg ctcccgaaga 3181 tcctggggga ggtgggcatg aggacgctgg gagagtacca cggctacgac cccggcatca 3241 atgctggcat cttcaacgcc ttcgccaccg cggccttcag gtttggccac acgcttgtca 3301 acccactgct ttaccggctg gacgagaact tccagcccat tgcacaagat cacctccccc 3361 ttcacaaagc tttcttctct cccttccgga ttgtgaatga gggcggcatc gatccgcttc 3421 tcagggggct gttcggggtg gcggggaaaa tgcgtgtgcc ctcgcagctg ctgaacacgg 3481 agctcacgga gcggctgttc tccatggcac acacggtggc tctggacctg gcggccatca 3541 acatccagcg gggccgggac cacgggatcc caccctacca cgactacagg gtctactgca 3601 atctatcggc ggcacacacg ttcgaggacc tgaaaaatga gattaaaaac cctgagatcc 3661 gggagaaact gaaaaggttg tatggctcga cactcaacat cgacctgttt ccggcgctcg 3721 tggtggagga cctggtgcct ggcagccggc tgggccccac cctgatgtgt cttctcagca 3781 cacagttcaa gcgcctgcga gatggggaca ggttgtggta tgagaaccct ggggtgttct 3841 ccccggccca gctgactcag atcaagcaga cgtcgctggc caggatccta tgcgacaacg 3901 cggacaacat cacccgggtg cagagcgacg tgttcagggt ggcggagttc cctcacggct 3961 acggcagctg tgacgagatc cccagggtag acctccgggt gtggcaggac tgctgtgaag 4021 actgtaggac cagggggcag ttcaatgcct tttcctatca tttccgaggc agacggtctc 4081 ttgagttcag ctaccaggag gacaagccga ccaagaaaac aagaccacgg aaaataccca 4141 gtgttgggag acagggggaa catctcagca acagcacctc agccttcagc acacgcTCAG 4201 ATGCATCTGG GACAAATGAC TTCAGAGAGT TTGTTCTGGA AATGCAGAAG ACCATCACAG 4261 ACCTCAGAAC ACAGATAAAG AAACTTGAAT CACGGCTCAG TACCACAGAG TGCGTGGATG 4321 CCGGGGGCGA ATCTCACGCC AACAACACCA AGTGGAAAAA AGATGCATGC ACCATTTGTG 4381 AATGCAAAGA CGGGCAGGTC ACCTGCTTCG TGGAAGCTTG CCCCCCTGCC ACCTGTGCTG 4441 TCCCCGTGAA CATCCCAGGG GCCTGCTGTC CAGTCTGCTT ACAGAAGAGG GCGGAGGAAA 4501 AGCCCTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 4561 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 4621 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCGCCC CTTGGAGTGA TACTTGGTAC 4681 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt