Construct: ORF TRCN0000489806
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020859.1_s317c1
- DNA Barcode:
- TCTAACGTCTTTTTCTTTCCTTAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LPAR2 (9170)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489806
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | NM_004720.6 | 99.9% | 100% | 714C>T |
| 2 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | XM_017027468.2 | 99.8% | 100% | 714C>T;864C>A |
| 3 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | XM_017027469.1 | 99.8% | 100% | 714C>T;864C>A |
| 4 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | XM_024451774.1 | 99.8% | 100% | 714C>T;864C>A |
| 5 | mouse | 53978 | Lpar2 | lysophosphatidic acid recep... | NM_020028.3 | 85.7% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1125
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtcatc atgggccagt gctactacaa cgagaccatc ggcttcttct 121 ataacaacag tggcaaagag ctcagctccc actggcggcc caaggatgtg gtcgtggtgg 181 cactggggct gaccgtcagc gtgctggtgc tgctgaccaa tctgctggtc atagcagcca 241 tcgcctccaa ccgccgcttc caccagccca tctactacct gctcggcaat ctggccgcgg 301 ctgacctctt cgcgggcgtg gcctacctct tcctcatgtt ccacactggt ccccgcacag 361 cccgactttc acttgagggc tggttcctgc ggcagggctt gctggacaca agcctcactg 421 cgtcggtggc cacactgctg gccatcgccg tggagcggca ccgcagtgtg atggccgtgc 481 agctgcacag ccgcctgccc cgtggccgcg tggtcatgct cattgtgggc gtgtgggtgg 541 ctgccctggg cctggggctg ctgcctgccc actcctggca ctgcctctgt gccctggacc 601 gctgctcacg catggcaccc ctgctcagcc gctcctattt ggccgtctgg gctctgtcga 661 gcctgcttgt cttcctgctc atggtggctg tgtacacccg cattttcttc TACGTGCGGC 721 GGCGAGTGCA GCGCATGGCA GAGCATGTCA GCTGCCACCC CCGCTACCGA GAGACCACGC 781 TCAGTCTGGT CAAGACTGTT GTCATCATCC TGGGGGCGTT CGTGGTCTGC TGGACACCAG 841 GCCAGGTGGT ACTGCTCCTG GATGGTTTAG GCTGTGAGTC CTGCAATGTC CTGGCTGTAG 901 AAAAGTACTT CCTACTGTTG GCCGAGGCCA ACTCACTGGT CAATGCTGCT GTGTACTCTT 961 GCCGAGATGC TGAGATGCGC CGCACCTTCC GCCGCCTTCT CTGCTGCGCG TGCCTCCGCC 1021 AGTCCACCCG CGAGTCTGTC CACTATACAT CCTCTGCCCA GGGAGGTGCC AGCACTCGCA 1081 TCATGCTTCC CGAGAACGGC CACCCACTGA TGGACTCCAC CCTTTAGAAC CCAGCTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGATCTAACG TCTTTTTCTT TCCTTATACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt