Construct: ORF TRCN0000489822
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021206.1_s317c1
- DNA Barcode:
- CTTTTACAAGCTCTGGTGAGACGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OR51E2 (81285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489822
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81285 | OR51E2 | olfactory receptor family 5... | NM_030774.4 | 99.8% | 100% | 777G>T |
| 2 | mouse | 170639 | Olfr78 | olfactory receptor 78 | NM_001168503.1 | 86.8% | 92.8% | (many diffs) |
| 3 | mouse | 170639 | Olfr78 | olfactory receptor 78 | NM_130866.4 | 86.8% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1032
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagttcc tgcaacttca cacatgccac ctttgtgctt attggtatcc 121 caggattaga gaaagcccat ttctgggttg gcttccccct cctttccatg tatgtagtgg 181 caatgtttgg aaactgcatc gtggtcttca tcgtaaggac ggaacgcagc ctgcacgctc 241 cgatgtacct ctttctctgc atgcttgcag ccattgacct ggccttatcc acatccacca 301 tgcctaagat ccttgccctt ttctggtttg attcccgaga gattagcttt gaggcctgtc 361 ttacccagat gttctttatt catgccctct cagccattga atccaccatc ctgctggcca 421 tggcctttga ccgttatgtg gccatctgcc acccactgcg ccatgctgca gtgctcaaca 481 atacagtaac agcccagatt ggcatcgtgg ctgtggtccg cggatccctc ttttttttcc 541 cactgcctct gctgatcaag cggctggcct tctgccactc caatgtcctc tcgcactcct 601 ATTGTGTCCA CCAGGATGTA ATGAAGTTGG CCTATGCAGA CACTTTGCCC AATGTGGTAT 661 ATGGTCTTAC TGCCATTCTG CTGGTCATGG GCGTGGACGT AATGTTCATC TCCTTGTCCT 721 ATTTTCTGAT AATACGAACG GTTCTGCAAC TGCCTTCCAA GTCAGAGCGG GCCAAGGCCT 781 TTGGAACCTG TGTGTCACAC ATTGGTGTGG TACTCGCCTT CTATGTGCCA CTTATTGGCC 841 TCTCAGTTGT ACACCGCTTT GGAAACAGCC TTCATCCCAT TGTGCGTGTT GTCATGGGTG 901 ACATCTACCT GCTGCTGCCT CCTGTCATCA ATCCCATCAT CTATGGTGCC AAAACCAAAC 961 AGATCAGAAC ACGGGTGCTG GCTATGTTCA AGATCAGCTG TGACAAGGAC TTGCAGGCTG 1021 TGGGAGGCAA GTAGAACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTTTTACAAG CTCTGGTGAG 1201 ACGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt