Construct: ORF TRCN0000489831
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019194.1_s317c1
- DNA Barcode:
- CATATGCTTGTGTTTTGGCCGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRY1 (1407)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489831
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1407 | CRY1 | cryptochrome circadian regu... | NM_004075.5 | 99.9% | 100% | 636T>C |
| 2 | human | 1407 | CRY1 | cryptochrome circadian regu... | XM_017018832.2 | 93.8% | 91.3% | (many diffs) |
| 3 | human | 1407 | CRY1 | cryptochrome circadian regu... | XM_024448844.1 | 92.6% | 87.2% | 636T>C;1656_1729del;1833_1896del |
| 4 | human | 1407 | CRY1 | cryptochrome circadian regu... | XM_024448845.1 | 86.9% | 79.3% | (many diffs) |
| 5 | mouse | 12952 | Cry1 | cryptochrome 1 (photolyase-... | NM_007771.3 | 86.3% | 93.3% | (many diffs) |
| 6 | mouse | 12952 | Cry1 | cryptochrome 1 (photolyase-... | XM_011243348.1 | 79% | 83.7% | (many diffs) |
| 7 | mouse | 12952 | Cry1 | cryptochrome 1 (photolyase-... | XM_011243349.1 | 62.2% | 67.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1833
- ORF length:
- 1758
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggg gtgaacgccg tgcactggtt ccgaaagggg ctccggctcc 121 acgacaaccc cgccctgaag gagtgcattc agggcgccga caccatccgc tgcgtctaca 181 tcctggaccc ctggttcgcc ggctcctcca atgtgggcat caacaggtgg cgatttttgc 241 ttcagtgtct tgaggatctt gatgccaatc tacgaaaatt aaactcccgt ctgtttgtga 301 ttcgtggaca accagcagat gtgtttccca ggcttttcaa ggaatggaac attactaaac 361 tttcaattga gtatgattct gagccctttg gaaaggaacg agacgcagct attaagaaac 421 tggcaactga agctggagta gaagtcattg taagaatttc acatacatta tatgacctag 481 acaagatcat agaactcaat ggtggacaac cgcctctaac ttataaaaga ttccagactc 541 tcatcagcaa aatggaacca ctagagatac cagtagagac aattacttca gaagtgatag 601 aaaagtgcac aactcctctg tctgatgacc atgatgagaa atatggagtc ccttcactgg 661 aagagctagg ttttgataca gatggcttat cctctgcagt gtggccaggc ggagaaactg 721 aagcacttac tcgtttggaa aggcatttgg aaagaaaagc ttgggtggca aattttgaaa 781 gacctcgaat gaatgcgaat tctctgcttg caagccctac tggacttagt ccttatctcc 841 gatttggttg tttgtcatgt cgactgtttt acttcaaact aacagatctc tacaaaaagg 901 taaagaagaa cagttcccct cccctttccc tttatgggca actgttatgg cgtgaatttt 961 tctatacagc agcaacaaat aatccacgct ttgataaaat ggaaggaaac cctatctgtg 1021 ttcagattcc ttgggataaa aatcctgagg ctttagccaa atgggcggaa ggccggacag 1081 gctttccatg gattgatgcc atcatgacac agcttcgtca ggagggttgg attcatcatc 1141 tagccaggca tgcagttgct tgcttcctga cacgagggga cctgtggatt agttgggaag 1201 aaggaatgaa ggtatttgaa gaattattgc ttgatgcaga ttggagcata aatgctggaa 1261 gttggatgtg gctgtcttgt agttcctttt ttcaacagtt ttttcactgc tattgccctg 1321 ttggttttgg taggagaaca gatcccaatg gagactatat caggcgttat ttgcctgtcc 1381 taagaggctt ccctgcaaaa tatatctatg atccctggaa tgcaccagaa ggtatccaaa 1441 aggtagCCAA ATGTTTGATA GGAGTTAATT ATCCTAAACC AATGGTGAAC CATGCTGAGG 1501 CAAGCCGTTT GAATATCGAA AGGATGAAAC AGATCTATCA GCAGCTTTCA CGATATAGAG 1561 GACTAGGTCT TCTGGCATCA GTACCTTCTA ATCCTAATGG GAATGGAGGC TTCATGGGAT 1621 ATTCTGCAGA AAATATCCCA GGTTGTAGCA GCAGTGGAAG TTGCTCTCAA GGGAGTGGTA 1681 TTTTACACTA TGCTCATGGC GACAGTCAGC AAACTCACCT GTTGAAGCAA GGAAGAAGCT 1741 CCATGGGCAC TGGTCTCAGT GGTGGGAAAC GTCCTAGTCA GGAAGAGGAC ACACAGAGTA 1801 TTGGTCCTAA AGTCCAGAGA CAGAGCACTA ATGACCCAGC TTTCTTGTAC AAAGTGGTTG 1861 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1921 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1981 TATGCTTGTG TTTTGGCCGG AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2041 ttgtgaaaga tt