Construct: ORF TRCN0000489832
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019156.1_s317c1
- DNA Barcode:
- TCATACGTCGTTGTAGGAGTTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRB1 (575)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489832
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | NM_001702.3 | 89.8% | 87.8% | (many diffs) |
| 2 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013696.1 | 87.9% | 86% | (many diffs) |
| 3 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013695.1 | 87.6% | 85.7% | (many diffs) |
| 4 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013694.1 | 87.3% | 85.4% | (many diffs) |
| 5 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013692.1 | 85.6% | 83.7% | (many diffs) |
| 6 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013691.1 | 85.5% | 83.7% | (many diffs) |
| 7 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013693.1 | 85.4% | 83.5% | (many diffs) |
| 8 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013697.1 | 82.2% | 80.4% | (many diffs) |
| 9 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_011517202.2 | 81.4% | 79.6% | 3117G>C;3397_3398ins100;3483_3484ins690 |
| 10 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | NM_174991.3 | 79.5% | 83.6% | (many diffs) |
| 11 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_011245404.1 | 79.3% | 83.3% | (many diffs) |
| 12 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_011245403.1 | 77.9% | 81.9% | (many diffs) |
| 13 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_006520269.2 | 77.7% | 81.7% | (many diffs) |
| 14 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_006520270.2 | 77.6% | 81.6% | (many diffs) |
| 15 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_006520271.2 | 77.5% | 81.5% | (many diffs) |
| 16 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_011245406.2 | 70.6% | 75.3% | (many diffs) |
| 17 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_011245405.1 | 70% | 73.5% | (many diffs) |
| 18 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XR_383967.3 | 65.5% | (many diffs) | |
| 19 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XR_383969.2 | 59.3% | (many diffs) | |
| 20 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XR_875215.1 | 59.1% | (many diffs) | |
| 21 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_006520276.2 | 44.3% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 4350
- ORF length:
- 4275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgagg ggccaggccg ccgccccggg ccccgtctgg atcctcgccc 121 cgctgctact gctgctgctg ctgctgggac gccgcgcgcg ggcggccgcc ggagcagacg 181 cggggcccgg gcccgagccg tgcgccacgc tggtgcaggg aaagttcttc ggctacttct 241 ccgcggccgc cgtgttcccg gccaacgcct cgcgctgctc ctggacgcta cgcaacccgg 301 acccgcggcg ctacactctc tacatgaagg tggccaaggc gcccgtgccc tgcagcggcc 361 ccggccgcgt gcgcacctac cagttcgact ccttcctcga gtccacgcgc acctacctgg 421 gcgtggagag cttcgacgag gtgctgcggc tctgcgaccc ctccgcaccc ctggccttcc 481 tgcaggccag caagcagttc ctgcagatgc ggcgccagca gccgccccag cacgacgggc 541 tccggccccg ggccgggccg ccgggcccca ccgacgactt ctccgtggag tacctggtgg 601 tggggaaccg caaccccagc cgtgccgcct gccagatgct gtgccgctgg ctggacgcgt 661 gtctggccgg tagtcgcagc tcgcacccct gcgggatcat gcagaccccc tgcgcctgcc 721 tgggcggcga ggcgggcggc cctgccgcgg gacccctggc cccccgcggg gatgtctgct 781 tgagagatgc ggtggctggt ggccctgaaa actgcctcac cagcctgacc caggaccggg 841 gcgggcacgg cgccacaggc ggctggaagc tgtggtccct gtggggcgaa tgcacgcggg 901 actgcggggg aggcctccag acgcggacgc gcacctgcct gcccgcgccg ggcgtggagg 961 gcggcggctg cgagggggtg ctggaggagg gtcgccagtg caaccgcgag gcctgcggcc 1021 ccgctgggcg caccagctcc cggagccagt ccctgcggtc cacagatgcc cggcggcgcg 1081 aggagctggg ggacgagctg cagcagtttg ggttcccagc cccccagacc ggtgacccag 1141 cagccgagga gtggtccccg tggagcgtgt gctccagcac ctgcggcgag ggctggcaga 1201 cccgcacgcg cttctgcgtg tcctcctcct acagcacgca gtgcagcgga cccctgcgcg 1261 agcagcggct gtgcaacaac tctgccgtgt gcccagtgca tggtgcctgg gatgagtggt 1321 cgccctggag cctctgctcc agcacctgtg gccgtggctt tcgggatcgc acgcgcacct 1381 gcaggccccc ccagtttggg ggcaacccct gtgagggccc tgagaagcaa accaagttct 1441 gcaacattgc cctgtgccct ggccgggcag tggatggaaa ctggaatgag tggtcgagct 1501 ggagcgcctg ctccgccagc tgctcccagg gccgacagca gcgcacgcgt gaatgcaacg 1561 ggccttccta cgggggtgcg gagtgccagg gccactgggt ggagacccga gactgcttcc 1621 tgcagcagtg cccagtggat ggcaagtggc aggcctgggc gtcatggggc agttgcagcg 1681 tcacgtgtgg ggctggcagc cagcgacggg agcgtgtctg ctctgggccc ttcttcgggg 1741 gagcagcctg ccagggcccc caggatgagt accggcagtg cggcacccag cggtgtcccg 1801 agccccatga gatctgtgat gaggacaact ttggtgctgt gatctggaag gagaccccag 1861 cgggagaggt ggctgctgtc cggtgtcccc gcaacgccac aggactcatc ctgcgacggt 1921 gtgagctgga cgaggaaggc atcgcctact gggagccccc cacctacatc cgctgtgttt 1981 ccattgacta cagaaacatc cagatgatga cccgggagca cctggccaag gctcagcgag 2041 ggctgcctgg ggagggggtc tcggaggtca tccagacact ggtggagatc tctcaggacg 2101 ggaccagcta cagtggggac ctgctgtcca ccatcgatgt cctgaggaac atgacagaga 2161 ttttccggag agcgtactac agccccaccc ctggggacgt acagaacttt gtccagatcc 2221 ttagcaacct gttggcagag gagaatcggg acaagtggga ggaggcccag ctggcgggcc 2281 ccaacgccaa ggagctgttc cggctggtgg aggactttgt ggacgtcatc ggcttccgca 2341 tgaaggacct gagggatgca taccaggtga cagacaacct ggttctcagc atccataagc 2401 tcccagccag cggagccact gacatcagct tccccatgaa gggctggcgg gccacgggtg 2461 actgggccaa ggtgccagag gacagggtca ctgtgtccaa gagtgtcttc tccacggggc 2521 tgacagaggc cgatgaagca tccgtgtttg tggtgggcac cgtgctctac aggaacctgg 2581 gcagcttcct ggccctgcag aggaacacga ccgtcctgaa ttctaaggtg atctccgtga 2641 ctgtgaaacc cccgcctcgc tccctgcgca cacccttgga gatcgagttt gcccacatgt 2701 ataatggcac caccaaccag acctgtatcc tgtgggatga gacggatgta ccctcctcct 2761 ccgccccccc gcagctcggg ccctggtcgt ggcgcggctg ccgcacggtg cccctcgacg 2821 ccctccggac gcgctgcctc tgtgaccggc tctccacctt cgccatctta gcccagctca 2881 gcgccgacgc gaacatggag aaggcgactc tgccgtcggt gacgctcatc gtgggctgtg 2941 gcgtgtcctc tctcaccctg ctcatgctgg tcatcatcta cgtgtccgtg tggaggtaca 3001 ttcgctcaga gcgttctgtc atcctcatca acttctgcct gtccatcatc tcctccaatg 3061 ccctcatcct catcgggcag acccagaccc gcaacaaggt ggtgtgcacg ctggtggccg 3121 ccttcctgca cttcttcttc ctgtcctcct tctgctgggt gctcaccgag gcctggcagt 3181 cctacatggc cgtgacgggc cacctccgga accgcctcat ccgcaagcgc ttcctctgcc 3241 tgggctgggg gctccctgca ctggttgtgg ccatttctgt gggattcacc aaggccaaag 3301 ggtacagcac catgaactac tgctggctct ccctggaggg gggactgctc tatgccttcg 3361 tgggacctgc cgctgccgtt gtgctggtga acatggtcat tgggatcctg gtgttcaaca 3421 agctcgtgtc caaagacggc atcacggaca agaagctgaa ggagcgggca ggggcctccc 3481 tgtggagctc ctgcgtggtg ctgccgctgc tggcgctgac ctggatgtcg gctgtgctcg 3541 ccgtcaccga ccgccgctcc gccctcttcc agatcctctt cgctgtcttc gactcgctgg 3601 agggcttcgt catcgtcatg gtgcactgta tcctccgtag agaggtccag gacgctgtga 3661 aatgccgtgt ggttgaccgg caggaggagg gcaacgggga ctcagggggc tccttccaga 3721 acggccacgc ccagctcatg accgacttcg agaaggacgt ggatctggcc tgtagatcag 3781 tgctgaacaa ggacatcgcg gcctgccgca ctgccaccat cacgggcaca ctgaagcggc 3841 cgtctctgcc cgaggaggag aagctgaagc tggcccatgc caaggggccg cccaccaatt 3901 tcaacagcct gccggccaac gtgtccaagc tgcacctgca cggctcaccc cgctatcccg 3961 gcgggcccct gcccgacttc cccaaccact cactgaccct caagagggac aaggcgccca 4021 agtcctccTT CGTCGGTGAC GGGGACATCT TCAAGAAGCT GGACTCGGAT CTGAGCCGGG 4081 CCCAGGAGAA GGCTCTGGAC ACGAGCTACG TGATCCTGCC CACGGCCACG GCCACGCTGC 4141 GGCCCAAGCC CAAGGAGGAG CCCAAGTACA GCATCCACAT TGACCAGATG CCGCAGACCC 4201 GCCTCATCCA CCTCAGCAGC CCCTGCCCCC ACCGCCCAAT CTGGAGCCGG CACCCCCCAG 4261 CCTGGGGGAT CCCGGGGAGC CTGCCGCCCA TCCGGGACCC AGCACGGGGC CCAGCACCAA 4321 GAACGAGAAT GTCGCCACCT TGTCTGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 4381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 4441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCATACGT 4501 CGTTGTAGGA GTTGAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4561 aagatt