Construct: ORF TRCN0000489837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021900.1_s317c1
- DNA Barcode:
- TACGGCAAGTGGATTATCAAACTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GNAI3 (2773)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489837
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2773 | GNAI3 | G protein subunit alpha i3 | NM_006496.4 | 100% | 100% | |
| 2 | human | 2770 | GNAI1 | G protein subunit alpha i1 | NM_002069.6 | 80.9% | 93.7% | (many diffs) |
| 3 | human | 2770 | GNAI1 | G protein subunit alpha i1 | NM_001256414.2 | 68.3% | 79.3% | (many diffs) |
| 4 | mouse | 14679 | Gnai3 | guanine nucleotide binding ... | NM_010306.3 | 93.5% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1134
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggctgc acgttgagcg ccgaagacaa ggcggcagtg gagcgaagca 121 agatgatcga ccgcaactta cgggaggacg gggaaaaagc ggccaaagaa gtgaagctgc 181 tgctactcgg tgctggagaa tctggtaaaa gcaccattgt gaaacagatg aaaatcattc 241 atgaggatgg ctattcagag gatgaatgta aacaatataa agtagttgtc tacagcaata 301 ctatacagtc catcattgca atcataagag ccatgggacg gctaaagatt gactttgggg 361 aagctgccag ggcagatgat gcccggcaat tatttgtttt agctggcagt gctgaagaag 421 gagtcatgac tccagaacta gcaggagtga ttaaacggtt atggcgagat ggtggggtac 481 aagcttgctt cagcagatcc agggaatatc agctcaatga ttctgcttca tattatctaa 541 atgatctgga tagaatatcc cagtctaact acattccaac tcagcaagat gttcttcgga 601 cgagagtgaa gaccacaggc attgtagaaa cacatttcac cttcaaagac ctatacttca 661 agatgtttga tgtaggtggc caaagatcag aacgaaaaaa gtggattcac tgttttgagg 721 gagtgacagc aattatcttc tgtgtggccc TCAGTGATTA TGACCTTGTT CTGGCTGAGG 781 ACGAGGAGAT GAACCGAATG CATGAAAGCA TGAAACTGTT TGACAGCATT TGTAATAACA 841 AATGGTTTAC AGAAACTTCA ATCATTCTCT TCCTTAACAA GAAAGACCTT TTTGAGGAAA 901 AAATAAAGAG GAGTCCGTTA ACTATCTGTT ATCCAGAATA CACAGGTTCC AATACATATG 961 AAGAGGCAGC TGCCTATATT CAATGCCAGT TTGAAGATCT GAACAGAAGA AAAGATACCA 1021 AGGAGATCTA TACTCACTTC ACCTGTGCCA CAGACACGAA GAATGTGCAG TTTGTTTTTG 1081 ATGCTGTTAC AGATGTCATC ATTAAAAACA ACTTAAAGGA ATGTGGACTT TATTGAGACC 1141 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GATACGGCAA GTGGATTATC AAACTGACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt