Transcript: Human NM_002069.6

Homo sapiens G protein subunit alpha i1 (GNAI1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
GNAI1 (2770)
Length:
10083
CDS:
331..1395

Additional Resources:

NCBI RefSeq record:
NM_002069.6
NBCI Gene record:
GNAI1 (2770)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002069.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425261 CAAACCAAATGAGTACTTATA pLKO_005 1425 3UTR 100% 13.200 18.480 N GNAI1 n/a
2 TRCN0000036471 CCAAATTACATCCCGACTCAA pLKO.1 823 CDS 100% 4.950 6.930 N GNAI1 n/a
3 TRCN0000036472 GAGGAGTGTAAACAATACAAA pLKO.1 520 CDS 100% 5.625 4.500 N GNAI1 n/a
4 TRCN0000341948 CAGTTTGAAGACCTCAATAAA pLKO_005 1246 CDS 100% 15.000 10.500 N Gnai1 n/a
5 TRCN0000424753 ATGATTCTGCAGCATACTATT pLKO_005 776 CDS 100% 13.200 9.240 N GNAI1 n/a
6 TRCN0000418209 TACTGAATGACAGACTATTAC pLKO_005 1781 3UTR 100% 13.200 9.240 N GNAI1 n/a
7 TRCN0000421531 TTAAAGCTGGGCTCTAGTATA pLKO_005 1615 3UTR 100% 13.200 9.240 N GNAI1 n/a
8 TRCN0000036469 CCAGTCAATTATTGCTATCAT pLKO.1 564 CDS 100% 5.625 3.938 N GNAI1 n/a
9 TRCN0000036473 ACGGCGATCATCTTCTGTGTA pLKO.1 985 CDS 100% 4.950 3.465 N GNAI1 n/a
10 TRCN0000036470 GCAGGATCAAACACATATGAA pLKO.1 1201 CDS 100% 0.000 0.000 N GNAI1 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 5799 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 5799 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002069.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06291 pDONR223 100% 99.7% 99.4% None 655A>G;819C>T;863C>A n/a
2 ccsbBroad304_06291 pLX_304 0% 99.7% 99.4% V5 655A>G;819C>T;863C>A n/a
3 TRCN0000471008 GATTTGGTAGTCACACGCTTACGT pLX_317 38% 99.7% 99.4% V5 655A>G;819C>T;863C>A n/a
4 ccsbBroadEn_00651 pDONR223 100% 80.9% 93.7% None (many diffs) n/a
5 ccsbBroad304_00651 pLX_304 0% 80.9% 93.7% V5 (many diffs) n/a
6 TRCN0000467869 TGTGAGGTTCTAACACACTTTTAT pLX_317 36.4% 80.9% 93.7% V5 (many diffs) n/a
7 TRCN0000489837 TACGGCAAGTGGATTATCAAACTG pLX_317 36.3% 80.9% 93.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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