Transcript: Human NM_006496.4

Homo sapiens G protein subunit alpha i3 (GNAI3), mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
GNAI3 (2773)
Length:
9044
CDS:
107..1171

Additional Resources:

NCBI RefSeq record:
NM_006496.4
NBCI Gene record:
GNAI3 (2773)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006496.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000036475 GCAGGAGTGATTAAACGGTTA pLKO.1 476 CDS 100% 4.050 5.670 N GNAI3 n/a
2 TRCN0000300354 GCAGGAGTGATTAAACGGTTA pLKO_005 476 CDS 100% 4.050 5.670 N GNAI3 n/a
3 TRCN0000036474 GCTCAATGATTCTGCTTCATA pLKO.1 547 CDS 100% 5.625 3.938 N GNAI3 n/a
4 TRCN0000310624 GCTCAATGATTCTGCTTCATA pLKO_005 547 CDS 100% 5.625 3.938 N GNAI3 n/a
5 TRCN0000036477 CTTCAATCATTCTCTTCCTTA pLKO.1 891 CDS 100% 4.950 3.465 N GNAI3 n/a
6 TRCN0000300356 CTTCAATCATTCTCTTCCTTA pLKO_005 891 CDS 100% 4.950 3.465 N GNAI3 n/a
7 TRCN0000036476 GCCCTCAGTGATTATGACCTT pLKO.1 782 CDS 100% 2.640 1.848 N GNAI3 n/a
8 TRCN0000300355 GCCCTCAGTGATTATGACCTT pLKO_005 782 CDS 100% 2.640 1.848 N GNAI3 n/a
9 TRCN0000036478 CTTAAAGGAATGTGGACTTTA pLKO.1 1147 CDS 100% 1.320 0.924 N GNAI3 n/a
10 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 5387 3UTR 100% 4.950 2.475 Y n/a
11 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 6087 3UTR 100% 4.050 2.025 Y P3H4 n/a
12 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 6087 3UTR 100% 4.050 2.025 Y ORAI2 n/a
13 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 6087 3UTR 100% 4.050 2.025 Y P3H4 n/a
14 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3743 3UTR 100% 5.625 2.813 Y KLHL30 n/a
15 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 6215 3UTR 100% 4.950 2.475 Y DCAF11 n/a
16 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3743 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006496.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00651 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00651 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000467869 TGTGAGGTTCTAACACACTTTTAT pLX_317 36.4% 100% 100% V5 n/a
4 TRCN0000489837 TACGGCAAGTGGATTATCAAACTG pLX_317 36.3% 100% 100% V5 (not translated due to prior stop codon) n/a
5 ccsbBroadEn_06291 pDONR223 100% 80.9% 93.2% None (many diffs) n/a
6 ccsbBroad304_06291 pLX_304 0% 80.9% 93.2% V5 (many diffs) n/a
7 TRCN0000471008 GATTTGGTAGTCACACGCTTACGT pLX_317 38% 80.9% 93.2% V5 (many diffs) n/a
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