Construct: ORF TRCN0000489843
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021123.1_s317c1
- DNA Barcode:
- AGAATTGCATCTTTGGTCTGTATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CRY2 (1408)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489843
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1408 | CRY2 | cryptochrome circadian regu... | NM_021117.5 | 100% | 100% | |
| 2 | human | 1408 | CRY2 | cryptochrome circadian regu... | NM_001127457.2 | 89.3% | 88% | (many diffs) |
| 3 | mouse | 12953 | Cry2 | cryptochrome 2 (photolyase-... | NM_009963.4 | 90.2% | 94.2% | (many diffs) |
| 4 | mouse | 12953 | Cry2 | cryptochrome 2 (photolyase-... | XM_017315281.1 | 90.2% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1851
- ORF length:
- 1779
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggcg actgtggcga cggcggcagc tgtggccccg gcgccagcgc 121 ccggcacgga cagcgcctct tcggtgcact ggttccgcaa agggctgcga ctccacgaca 181 acccggcgtt gctggcggcc gtgcgcgggg cgcgctgcgt gcgctgcgtt tacattctcg 241 acccgtggtt cgcggcctcc tcctcagtcg ggatcaaccg atggaggttc ctacttcagt 301 ctctggaaga tttggacaca agtttaagga aactgaactc ccgcctgttt gtagtccggg 361 gacagccagc cgacgtgttc ccaaggctgt tcaaggaatg gggagtgacc cgcttgacct 421 ttgaatatga ctctgaaccc tttgggaaag aacgggatgc agccatcatg aagatggcca 481 aggaggctgg tgtggaagta gtgacggaga attctcatac cctctatgac ctggacagga 541 tcattgagct gaatgggcag aagccacccc ttacatacaa gcgctttcag gccatcatca 601 gccgcatgga gctgcccaag aagccagtgg gcttggtgac cagccagcag atggagagct 661 gcagggccga gatccaggag aaccacgacg agacctacgg cgtgccctcc ctggaggagc 721 tggggttccc cactgaagga cttggtccag ctgtctggca gggaggagag acagaagctc 781 tggcccgcct ggataagcac ttggaacgga aggcctgggt tgccaactat gagagacccc 841 gaatgaacgc caactccctc ctggccagcc ccacaggcct cagcccctac ctgcgctttg 901 gttgtctctc ctgccgcctc ttctactacc gcctgtggga cctgtataaa aaggtgaagc 961 ggaacagcac acctcccctc tccctatttg ggcaactcct atggcgagag ttcttctaca 1021 cggcagctac caacaacccc aggtttgacc gcatggaggg gaaccccatc tgcatccaga 1081 tcccctggga ccgcaatcct gaggccctgg ccaagtgggc tgagggcaag acaggcttcc 1141 cttggattga tgccatcatg acccaactga ggcaggaggg ctggatccac cacctggccc 1201 ggcatgccgt ggcctgcttc ctgacccgcg gggacctctg ggtcagctgg gagagcgggg 1261 tccgggtatt tgatgagctg ctcctggatg cagatttcag cgtgaacgca ggcagctgga 1321 tgtggctgtc ctgcagtgct ttcttccagc agttcttcca ctgctactgc cctgtgggct 1381 ttggccgtcG CACGGACCCC AGTGGGGACT ACATCAGGCG ATACCTGCCC AAATTGAAAG 1441 CGTTCCCCTC TCGATACATC TATGAGCCCT GGAATGCCCC AGAGTCAATT CAGAAGGCAG 1501 CCAAGTGCAT CATTGGTGTG GACTACCCAC GGCCCATCGT CAACCATGCC GAGACCAGCC 1561 GGCTTAACAT TGAACGAATG AAGCAGATTT ACCAGCAGCT TTCGCGCTAC CGGGGACTCT 1621 GTCTACTGGC ATCTGTCCCT TCCTGTGTGG AAGACCTCAG TCACCCTGTG GCAGAGCCCA 1681 GCTCGAGCCA GGCTGGCAGC ATGAGCAGTG CAGGCCCAAG ACCACTACCC AGTGGCCCAG 1741 CATCCCCCAA ACGCAAGCTG GAAGCAGCCG AGGAACCACC TGGTGAAGAA CTCAGCAAAC 1801 GGGCCCGGGT GGCAGAGTTG CCAACCCCAG AGCTGCCGAG CAAGGATGCC TAGAACCCAG 1861 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1921 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1981 TATCTTGTGG AAAGGACGAA GAATTGCATC TTTGGTCTGT ATCACGCGTT AAGTCgacaa 2041 tcaacctctg gattacaaaa tttgtgaaag att