Construct: ORF TRCN0000489844

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020966.1_s317c1
DNA Barcode:
ATCTCTAGAACCGTATCCGAGGAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CHRM3 (1131)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489844
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1131 CHRM3 cholinergic receptor muscar... NM_000740.3 100% 100%
2 human 1131 CHRM3 cholinergic receptor muscar... NM_001347716.2 100% 100%
3 human 1131 CHRM3 cholinergic receptor muscar... XM_005273032.3 100% 100%
4 human 1131 CHRM3 cholinergic receptor muscar... XM_011544041.2 100% 100%
5 human 1131 CHRM3 cholinergic receptor muscar... XM_011544043.2 100% 100%
6 human 1131 CHRM3 cholinergic receptor muscar... XM_011544044.2 100% 100%
7 human 1131 CHRM3 cholinergic receptor muscar... XM_011544046.2 100% 100%
8 human 1131 CHRM3 cholinergic receptor muscar... XM_011544047.2 100% 100%
9 human 1131 CHRM3 cholinergic receptor muscar... XM_017000152.2 100% 100%
10 human 1131 CHRM3 cholinergic receptor muscar... XM_017000153.1 100% 100%
11 human 1131 CHRM3 cholinergic receptor muscar... XM_017000154.1 100% 100%
12 human 1131 CHRM3 cholinergic receptor muscar... XM_017000155.1 100% 100%
13 human 1131 CHRM3 cholinergic receptor muscar... XM_017000156.1 100% 100%
14 human 1131 CHRM3 cholinergic receptor muscar... XM_017000157.2 100% 100%
15 human 1131 CHRM3 cholinergic receptor muscar... XM_017000158.1 100% 100%
16 human 1131 CHRM3 cholinergic receptor muscar... XM_017000159.1 100% 100%
17 human 1131 CHRM3 cholinergic receptor muscar... XM_017000160.2 100% 100%
18 human 1131 CHRM3 cholinergic receptor muscar... XM_017000161.1 100% 100%
19 human 1131 CHRM3 cholinergic receptor muscar... XM_017000162.1 100% 100%
20 human 1131 CHRM3 cholinergic receptor muscar... XM_017000163.2 100% 100%
21 mouse 12671 Chrm3 cholinergic receptor, musca... NM_033269.4 86.9% 91.6% (many diffs)
22 mouse 12671 Chrm3 cholinergic receptor, musca... XM_006516468.3 86.9% 91.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1842
ORF length:
1770
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaccttg cacaataaca gtacaacctc gcctttgttt ccaaacatca
121gctcctcctg gatacacagc ccctccgatg cagggctgcc cccgggaacc gtcactcatt
181tcggcagcta caatgtttct cgagcagctg gcaatttctc ctctccagac ggtaccaccg
241atgaccctct gggaggtcat accgtctggc aagtggtctt catcgctttc ttaacgggca
301tcctggcctt ggtgaccatc atcggcaaca tcctggtaat tgtgtcattt aaggtcaaca
361agcagctgaa gacggtcaac aactacttcc tcttaagcct ggcctgtgcc gatctgatta
421tcggggtcat ttcaatgaat ctgtttacga cctacatcat catgaatcga tgggccttag
481ggaacttggc ctgtgacctc tggcttgcca ttgactacgt agccagcaat gcctctgtta
541tgaatcttct ggtcatcagc tttgacagat acttttccat cacgaggccg ctcacgtacc
601gagccaaacg aacaacaaag agagccggtg tgatgatcgg tctggcttgg gtcatctcct
661ttgtcctttg ggctcctgcc atcttgttct ggcaatactt tgttggaaag agaactgtgc
721ctccgggaga gtgcttcatt cagttcctca gtgagcccac cattactttt ggcacagcca
781tcgctgcttt ttatatgcct gtcaccatta tgactatttt atactggagg atctataagg
841aaactgaaaa gcgtaccaaa gagcttgctg gcctgcaagc ctctgggaca gaggcagaga
901cagaaaactt tgtccacccc acgggcagtt ctcgaagctg cagcagttac gaacttcaac
961agcaaagcat gaaacgctcc aacaggagga agtatggccg ctgccacttc tggttcacaa
1021ccaagagctg gaaacccagc tccgagcaga tggaccaaga ccacagcagc agtgacagtt
1081ggaacaacaa tgatgctgct gcctccctgg agaactccgc ctcctccgac gaggaggaca
1141ttggctccga gacgagagcc atctactcca tcgtgctcaa gcttccgggt cacagcacca
1201tcctcaactc caccaagtta ccctcatcgg acaacctgca ggtgcctgag gaggagctgg
1261ggatggtgga cttggagagg aaagccgaca agctgcaggc ccagaagagc gtggacgatg
1321gaggcagttt tccaaaaagc ttctccaagc ttcccatcca gctagagtca gccgtggaca
1381cagctaagac ttctgacgtc aactcctcag tgggtaagag cacggccact ctacctctgt
1441ccttcaagga agcCACTCTG GCCAAGAGGT TTGCTCTGAA GACCAGAAGT CAGATCACTA
1501AGCGGAAAAG GATGTCCCTG GTCAAGGAGA AGAAAGCGGC CCAGACCCTC AGTGCGATCT
1561TGCTTGCCTT CATCATCACT TGGACCCCAT ACAACATCAT GGTTCTGGTG AACACCTTTT
1621GTGACAGCTG CATACCCAAA ACCTTTTGGA ATCTGGGCTA CTGGCTGTGC TACATCAACA
1681GCACCGTGAA CCCCGTGTGC TATGCTCTGT GCAACAAAAC ATTCAGAACC ACTTTCAAGA
1741TGCTGCTGCT GTGCCAGTGT GACAAAAAAA AGAGGCGCAA GCAGCAGTAC CAGCAGAGAC
1801AGTCGGTCAT TTTTCACAAG CGCGCACCCG AGCAGGCCTT GTAGGACCCA GCTTTCTTGT
1861ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1921AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1981GAAAGGACGA ATCTCTAGAA CCGTATCCGA GGACACGCGT TAAGTCgaca atcaacctct
2041ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.