Construct: ORF TRCN0000489844
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020966.1_s317c1
- DNA Barcode:
- ATCTCTAGAACCGTATCCGAGGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHRM3 (1131)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489844
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1131 | CHRM3 | cholinergic receptor muscar... | NM_000740.3 | 100% | 100% | |
| 2 | human | 1131 | CHRM3 | cholinergic receptor muscar... | NM_001347716.2 | 100% | 100% | |
| 3 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_005273032.3 | 100% | 100% | |
| 4 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544041.2 | 100% | 100% | |
| 5 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544043.2 | 100% | 100% | |
| 6 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544044.2 | 100% | 100% | |
| 7 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544046.2 | 100% | 100% | |
| 8 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544047.2 | 100% | 100% | |
| 9 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000152.2 | 100% | 100% | |
| 10 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000153.1 | 100% | 100% | |
| 11 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000154.1 | 100% | 100% | |
| 12 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000155.1 | 100% | 100% | |
| 13 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000156.1 | 100% | 100% | |
| 14 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000157.2 | 100% | 100% | |
| 15 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000158.1 | 100% | 100% | |
| 16 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000159.1 | 100% | 100% | |
| 17 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000160.2 | 100% | 100% | |
| 18 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000161.1 | 100% | 100% | |
| 19 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000162.1 | 100% | 100% | |
| 20 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000163.2 | 100% | 100% | |
| 21 | mouse | 12671 | Chrm3 | cholinergic receptor, musca... | NM_033269.4 | 86.9% | 91.6% | (many diffs) |
| 22 | mouse | 12671 | Chrm3 | cholinergic receptor, musca... | XM_006516468.3 | 86.9% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1842
- ORF length:
- 1770
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaccttg cacaataaca gtacaacctc gcctttgttt ccaaacatca 121 gctcctcctg gatacacagc ccctccgatg cagggctgcc cccgggaacc gtcactcatt 181 tcggcagcta caatgtttct cgagcagctg gcaatttctc ctctccagac ggtaccaccg 241 atgaccctct gggaggtcat accgtctggc aagtggtctt catcgctttc ttaacgggca 301 tcctggcctt ggtgaccatc atcggcaaca tcctggtaat tgtgtcattt aaggtcaaca 361 agcagctgaa gacggtcaac aactacttcc tcttaagcct ggcctgtgcc gatctgatta 421 tcggggtcat ttcaatgaat ctgtttacga cctacatcat catgaatcga tgggccttag 481 ggaacttggc ctgtgacctc tggcttgcca ttgactacgt agccagcaat gcctctgtta 541 tgaatcttct ggtcatcagc tttgacagat acttttccat cacgaggccg ctcacgtacc 601 gagccaaacg aacaacaaag agagccggtg tgatgatcgg tctggcttgg gtcatctcct 661 ttgtcctttg ggctcctgcc atcttgttct ggcaatactt tgttggaaag agaactgtgc 721 ctccgggaga gtgcttcatt cagttcctca gtgagcccac cattactttt ggcacagcca 781 tcgctgcttt ttatatgcct gtcaccatta tgactatttt atactggagg atctataagg 841 aaactgaaaa gcgtaccaaa gagcttgctg gcctgcaagc ctctgggaca gaggcagaga 901 cagaaaactt tgtccacccc acgggcagtt ctcgaagctg cagcagttac gaacttcaac 961 agcaaagcat gaaacgctcc aacaggagga agtatggccg ctgccacttc tggttcacaa 1021 ccaagagctg gaaacccagc tccgagcaga tggaccaaga ccacagcagc agtgacagtt 1081 ggaacaacaa tgatgctgct gcctccctgg agaactccgc ctcctccgac gaggaggaca 1141 ttggctccga gacgagagcc atctactcca tcgtgctcaa gcttccgggt cacagcacca 1201 tcctcaactc caccaagtta ccctcatcgg acaacctgca ggtgcctgag gaggagctgg 1261 ggatggtgga cttggagagg aaagccgaca agctgcaggc ccagaagagc gtggacgatg 1321 gaggcagttt tccaaaaagc ttctccaagc ttcccatcca gctagagtca gccgtggaca 1381 cagctaagac ttctgacgtc aactcctcag tgggtaagag cacggccact ctacctctgt 1441 ccttcaagga agcCACTCTG GCCAAGAGGT TTGCTCTGAA GACCAGAAGT CAGATCACTA 1501 AGCGGAAAAG GATGTCCCTG GTCAAGGAGA AGAAAGCGGC CCAGACCCTC AGTGCGATCT 1561 TGCTTGCCTT CATCATCACT TGGACCCCAT ACAACATCAT GGTTCTGGTG AACACCTTTT 1621 GTGACAGCTG CATACCCAAA ACCTTTTGGA ATCTGGGCTA CTGGCTGTGC TACATCAACA 1681 GCACCGTGAA CCCCGTGTGC TATGCTCTGT GCAACAAAAC ATTCAGAACC ACTTTCAAGA 1741 TGCTGCTGCT GTGCCAGTGT GACAAAAAAA AGAGGCGCAA GCAGCAGTAC CAGCAGAGAC 1801 AGTCGGTCAT TTTTCACAAG CGCGCACCCG AGCAGGCCTT GTAGGACCCA GCTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA ATCTCTAGAA CCGTATCCGA GGACACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt