Construct: ORF TRCN0000489868

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019489.1_s317c1
DNA Barcode:
CTATGATACTTTATCATCGTTCTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AKT2 (208)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489868
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 208 AKT2 AKT serine/threonine kinase 2 NM_001626.6 97.2% 97.1% (many diffs)
2 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526614.1 97.2% 97.1% (many diffs)
3 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526615.1 97.2% 97.1% (many diffs)
4 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526616.1 97.2% 97.1% (many diffs)
5 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526618.1 97.2% 97.1% (many diffs)
6 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526619.1 97.2% 97.1% (many diffs)
7 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526620.1 97.2% 97.1% (many diffs)
8 human 208 AKT2 AKT serine/threonine kinase 2 XM_017026470.2 97.2% 97.1% (many diffs)
9 human 208 AKT2 AKT serine/threonine kinase 2 XM_024451416.1 97.2% 97.1% (many diffs)
10 human 208 AKT2 AKT serine/threonine kinase 2 NM_001330511.1 88.5% 88.4% (many diffs)
11 human 208 AKT2 AKT serine/threonine kinase 2 XM_024451417.1 88.5% 88.4% (many diffs)
12 human 208 AKT2 AKT serine/threonine kinase 2 NM_001243027.3 84.7% 84.8% 0_1ins225;1257G>A
13 human 208 AKT2 AKT serine/threonine kinase 2 NM_001243028.3 84.7% 84.8% 0_1ins225;1257G>A
14 human 208 AKT2 AKT serine/threonine kinase 2 XM_011526622.2 80.3% 79.3% (many diffs)
15 mouse 11652 Akt2 thymoma viral proto-oncogene 2 NM_001110208.2 87.9% 95.3% (many diffs)
16 mouse 11652 Akt2 thymoma viral proto-oncogene 2 NM_001331108.1 87.9% 95.3% (many diffs)
17 mouse 11652 Akt2 thymoma viral proto-oncogene 2 NM_001331109.1 87.9% 95.3% (many diffs)
18 mouse 11652 Akt2 thymoma viral proto-oncogene 2 NM_007434.4 87.9% 95.3% (many diffs)
19 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539477.1 87.9% 95.3% (many diffs)
20 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539480.1 87.9% 95.3% (many diffs)
21 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539481.3 87.9% 95.3% (many diffs)
22 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539482.1 80.2% 86.6% (many diffs)
23 mouse 11652 Akt2 thymoma viral proto-oncogene 2 XM_006539483.1 80.2% 86.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
78
ORF end:
1560
ORF length:
1482
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg
61cggccgcccc cttcaccatg ggtagcaaca agagcaagcc caaggatgcc agccagcgga
121atgaggtgtc tgtcatcaaa gaaggctggc tccacaagcg tggtgaatac atcaagacct
181ggaggccacg gtatttcctg ctgaagagcg acggctcctt cattgggtac aaggagaggc
241ccgaggcccc tgatcagact ctacccccct taaacaactt ctccgtagca gaatgccagc
301tgatgaagac cgagaggccg cgacccaaca cctttgtcat acgctgcctg cagtggacca
361cagtcatcga gaggaccttc cacgtggatt ctccagacga gagggaggag tggatgcggg
421ccatccagat ggtcgccaac agcctcaagc agcgggcccc aggcgaggac cccatggact
481acaagtgtgg ctcccccagt gactcctcca cgactgagga gatggaagtg gcggtcagca
541aggcacgggc taaagtgacc atgaatgact tcgactatct caaactcctt ggcaagggaa
601cctttggcaa agtcatcctg gtgcgggaga aggccactgg ccgctactac gccatgaaga
661tcctgcggaa ggaagtcatc attgccaagg atgaagtcgc tcacacagtc accgagagcc
721gggtcctcca gaacaccagg cacccgttcc tcactgcgct gaagtatgcc ttccagaccc
781acgaccgcct gtgctttgtg atggagtatg ccaacggggg tgagctgttc ttccacctgt
841cccgggagcg tgtcttcaca gaggagcggg cccggtttta tggtgcagag attgtctcgg
901ctcttgagta cttgcactcg cgggacgtgg tataccgcga catcaagctg gaaaacctca
961tgctggacaa agatggccac atcaagatca ctgactttgg cctctgcaaa gagggcatca
1021gtgacggggc caccatgaaa accttctgtg ggaccccgga gtacctggcg cctgaggtgc
1081tggaggacaa tgactatggc cgggccgtgg actggtgggg gctgggtgtg gtcatgtacg
1141agatgatgtg cggccgcctg cccttctaca accaggacca cgagcgcctc ttcgagctca
1201tcctcatgga agagatccgc ttcccgcgca cgctcagccc cgaggccaag tccctgcttg
1261ctgggctgct taagaaggac cccaagcaga ggcttggtgg ggggcccagc gatgccaagg
1321aggtcatgga gcacaggttc ttcctcagca tcaactggca ggacgtggtc cagaagaagc
1381tcctgccaCC CTTCAAACCT CAGGTCACGT CCGAGGTCGA CACAAGGTAC TTCGATGATG
1441AATTTACCGC CCAGTCCATC ACAATCACAC CCCCTGACCG CTATGACAGC CTGGGCTTAC
1501TGGAGCTGGA CCAGCGGACC CACTTCCCCC AGTTCTCCTA CTCGGCCAGC ATCCGCGAAA
1561AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1621CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1681TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTATGATACT TTATCATCGT
1741TCTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

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