Construct: ORF TRCN0000489883
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020570.1_s317c1
- DNA Barcode:
- CATCGCAAGACCACGGGATCAGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MELK (9833)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489883
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9833 | MELK | maternal embryonic leucine ... | NM_014791.4 | 99.9% | 100% | 1686C>T |
| 2 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518076.2 | 99.9% | 100% | 1686C>T |
| 3 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518077.1 | 99.9% | 100% | 1686C>T |
| 4 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518078.2 | 99.9% | 100% | 1686C>T |
| 5 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518079.1 | 99.9% | 100% | 1686C>T |
| 6 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518080.1 | 95.4% | 95.5% | 834_835ins87;1599C>T |
| 7 | human | 9833 | MELK | maternal embryonic leucine ... | NM_001256689.1 | 95% | 93.2% | 0_1insATGAAAGATT;47_48ins86;1590C>T |
| 8 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518081.2 | 95% | 93.2% | 0_1insATGAAAGATT;47_48ins86;1590C>T |
| 9 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518082.2 | 95% | 93.2% | 0_1insATGAAAGATT;47_48ins86;1590C>T |
| 10 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518083.2 | 95% | 93.2% | 0_1insATGAAAGATT;47_48ins86;1590C>T |
| 11 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518084.2 | 95% | 93.2% | 0_1insATGAAAGATT;47_48ins86;1590C>T |
| 12 | human | 9833 | MELK | maternal embryonic leucine ... | NM_001256685.1 | 93.6% | 93.7% | 1051_1052ins123;1563C>T |
| 13 | human | 9833 | MELK | maternal embryonic leucine ... | NM_001256687.1 | 92.5% | 92.6% | 261_262ins144;1542C>T |
| 14 | human | 9833 | MELK | maternal embryonic leucine ... | NM_001256688.1 | 89% | 89% | 259_260ins213;1473C>T |
| 15 | human | 9833 | MELK | maternal embryonic leucine ... | NM_001256690.1 | 89% | 86.7% | 0_1insATGAAAGATT;46_47ins203;1473C>T |
| 16 | human | 9833 | MELK | maternal embryonic leucine ... | NM_001256691.1 | 87.6% | 85.8% | (many diffs) |
| 17 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518085.1 | 87.1% | 87.2% | 0_1ins249;1437C>T |
| 18 | human | 9833 | MELK | maternal embryonic leucine ... | NM_001256692.1 | 79.5% | 79.2% | (many diffs) |
| 19 | human | 9833 | MELK | maternal embryonic leucine ... | NR_046337.1 | 71.5% | (many diffs) | |
| 20 | human | 9833 | MELK | maternal embryonic leucine ... | NM_001256693.1 | 70.1% | 70.1% | 0_1ins582;1104C>T |
| 21 | human | 9833 | MELK | maternal embryonic leucine ... | XM_011518086.2 | 56.8% | 54.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2025
- ORF length:
- 1953
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaagat tatgatgaac ttctcaaata ttatgaatta catgaaacta 121 ttgggacagg tggctttgca aaggtcaaac ttgcctgcca tatccttact ggagagatgg 181 tagctataaa aatcatggat aaaaacacac tagggagtga tttgccccgg atcaaaacgg 241 agattgaggc cttgaagaac ctgagacatc agcatatatg tcaactctac catgtgctag 301 agacagccaa caaaatattc atggttcttg agtactgccc tggaggagag ctgtttgact 361 atataatttc ccaggatcgc ctgtcagaag aggagacccg ggttgtcttc cgtcagatag 421 tatctgctgt tgcttatgtg cacagccagg gctatgctca cagggacctc aagccagaaa 481 atttgctgtt tgatgaatat cataaattaa agctgattga ctttggtctc tgtgcaaaac 541 ccaagggtaa caaggattac catctacaga catgctgtgg gagtctggct tatgcagcac 601 ctgagttaat acaaggcaaa tcatatcttg gatcagaggc agatgtttgg agcatgggca 661 tactgttata tgttcttatg tgtggatttc taccatttga tgatgataat gtaatggctt 721 tatacaagaa gattatgaga ggaaaatatg atgttcccaa gtggctctct cccagtagca 781 ttctgcttct tcaacaaatg ctgcaggtgg acccaaagaa acggatttct atgaaaaatc 841 tattgaacca tccctggatc atgcaagatt acaactatcc tgttgagtgg caaagcaaga 901 atccttttat tcacctcgat gatgattgcg taacagaact ttctgtacat cacagaaaca 961 acaggcaaac aatggaggat ttaatttcac tgtggcagta tgatcacctc acggctacct 1021 atcttctgct tctagccaag aaggctcggg gaaaaccagt tcgtttaagg ctttcttctt 1081 tctcctgtgg acaagccagt gctaccccat tcacagacat caagtcaaat aattggagtc 1141 tggaagatgt gaccgcaagt gataaaaatt atgtggcggg attaatagac tatgattggt 1201 gtgaagatga tttatcaaca ggtgctgcta ctccccgaac atcacagttt accaagtact 1261 ggacagaatc aaatggggtg gaatctaaat cattaactcc agccttatgc agaacacctg 1321 caaataaatt aaagaacaaa gaaaatgtat atactcctaa gtctgctgta aagaatgaag 1381 agtactttat gtttcctgag ccaaagactc cagttaataa gaaccagcat aagagagaaa 1441 tactcactac gccaaatcgt tacactacac cctcaaaagc tagaaaccag tgcctgaaag 1501 aaactccaat taaaatacca gtaaattcaa caggaacaga caagttaatg acaggtgtca 1561 ttagccctga gaggcggtgc cgctcagtgg aattggatct caaccaagca catatggagg 1621 agactccaaa aagaaaggga gccaaagtgt ttgggagcct tgaaaggggg ttggataagg 1681 ttatcactgt gctcaccagg agcaaaagga agggttctgc cagagacggg cccagaagac 1741 taaagcttca ctataatgtG ACTACAACTA GATTAGTGAA TCCAGATCAA CTGTTGAATG 1801 AAATAATGTC TATTCTTCCA AAGAAGCATG TTGACTTTGT ACAAAAGGGT TATACACTGA 1861 AGTGTCAAAC ACAGTCAGAT TTTGGGAAAG TGACAATGCA ATTTGAATTA GAAGTGTGCC 1921 AGCTTCAAAA ACCCGATGTG GTGGGTATCA GGAGGCAGCG GCTTAAGGGC GATGCCTGGG 1981 TTTACAAAAG ATTAGTGGAA GACATCCTAT CTAGCTGCAA GGTATAAGCT AGCGACCCAG 2041 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2101 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2161 TATCTTGTGG AAAGGACGAC ATCGCAAGAC CACGGGATCA GTTACGCGTT AAGTCgacaa 2221 tcaacctctg gattacaaaa tttgtgaaag att