Construct: ORF TRCN0000489885
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020013.1_s317c1
- DNA Barcode:
- AACTGAGATCTTACTTGTTGGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPL (4023)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489885
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4023 | LPL | lipoprotein lipase | NM_000237.3 | 99.9% | 99.7% | 1425_1426insG |
| 2 | mouse | 16956 | Lpl | lipoprotein lipase | NM_008509.2 | 88% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1497
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagagcaaa gccctgctcg tgctgactct ggccgtgtgg ctccagagtc 121 tgaccgcctc ccgcggaggg gtggccgccg ccgaccaaag aagagatttt atcgacatcg 181 aaagtaaatt tgccctaagg acccctgaag acacagctga ggacacttgc cacctcattc 241 ccggagtagc agagtccgtg gctacctgtc atttcaatca cagcagcaaa accttcatgg 301 tgatccatgg ctggacggta acaggaatgt atgagagttg ggtgccaaaa cttgtggccg 361 ccctgtacaa gagagaacca gactccaatg tcattgtggt ggactggctg tcacgggctc 421 aggagcatta cccagtgtcc gcgggctaca ccaaactggt gggacaggat gtggcccggt 481 ttatcaactg gatggaggag gagtttaact accctctgga caatgtccat ctcttgggat 541 acagccttgg agcccatgct gctggcattg caggaagtct gaccaataag aaagtcaaca 601 gaattactgg cctcgatcca gctggaccta actttgagta tgcagaagcc ccgagtcgtc 661 tttctcctga tgatgcagat tttgtagacg tcttacacac attcaccaga gggtcccctg 721 gtcgaagcat tggaatccag aaaccagttg ggcatgttga catttacccg aatggaggta 781 cttttcagcc aggatgtaac attggagaag ctatccgcgt gattgcagag agaggacttg 841 gagatgtgga ccagctagtg aagtgctccc acgagcgctc cattcatctc ttcatcgact 901 ctctgttgaa tgaagaaaat ccaagtaagg cctacaggtg cagttccaag gaagcctttg 961 agaaagggct ctgcttgagt tgtagaaaga accgctgcaa caatctgggc tatgagatca 1021 ataaagtcag agccaaaaga agcagcaaaa tgtacctgaa gactcgttct cagatgccct 1081 acaaagtcTT CCATTACCAA GTAAAGATTC ATTTTTCTGG GACTGAGAGT GAAACCCATA 1141 CCAATCAGGC CTTTGAGATT TCTCTGTATG GCACCGTGGC CGAGAGTGAG AACATCCCAT 1201 TCACTCTGCC TGAAGTTTCC ACAAATAAGA CCTACTCCTT CCTAATTTAC ACAGAGGTAG 1261 ATATTGGAGA ACTACTCATG TTGAAGCTCA AATGGAAGAG TGATTCATAC TTTAGCTGGT 1321 CAGACTGGTG GAGCAGTCCC GGCTTCGCCA TTCAGAAGAT CAGAGTAAAA GCAGGAGAGA 1381 CTCAGAAAAA GGTGATCTTC TGTTCTAGGG AGAAAGTGTC TCATTTGCAG AAAGGAAAGG 1441 CACCTGCGGT ATTTGTGAAA TGCCATGACA AGTCTCTGAA TAAGAAGTCA GGCGACCCAG 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAA ACTGAGATCT TACTTGTTGG CACACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att