Construct: ORF TRCN0000489886
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019527.1_s317c1
- DNA Barcode:
- GGACTAAGTCGTAGTTTTATGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR5A (3361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489886
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3361 | HTR5A | 5-hydroxytryptamine recepto... | NM_024012.3 | 99.8% | 99.7% | 12T>A;1071_1072insG |
| 2 | mouse | 15563 | Htr5a | 5-hydroxytryptamine (seroto... | NM_008314.2 | 84.2% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1149
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggat ttaccagtga acctaacctc cttttccctc tccaccccct 121 cccctttgga gaccaaccac agcctcggca aagacgacct gcgccccagc tcgcccctgc 181 tctcggtctt cggagtgctt attctcacct tgctgggctt tctggtggcg gcgacgttcg 241 cctggaacct gctggtgctg gcgaccatcc tccgtgtacg caccttccac cgcgtgcccc 301 acaacctggt ggcatccatg gccgtctcgg atgtcctggt ggccgcgctg gtcatgccgc 361 tgagcctggt gcacgagctg tccgggcgcc gctggcagct aggtcggagg ctgtgccagc 421 tttggatcgc gtgcgacgtg ctttgctgca cggccagcat ctggaacgtg acggccatag 481 ccctggaccg ctactggtcc atcacgcgcc acatggaata cacgctccgc acccgcaagt 541 gcgtctccaa cgtcatgatc gcgctcacct gggcactctc cgctgtcatc tctctggccc 601 cgctgctttt tggctgggga gagacgtact ctgagggcag cgaggagtgc caggtaagcc 661 gcgagccttc ctacgccgtg ttctccaccg taggcgcctt ctaCCTGCCG CTCTGTGTGG 721 TGCTCTTCGT GTACTGGAAG ATCTACAAGG CTGCCAAGTT CCGCGTGGGC TCCAGGAAGA 781 CCAATAGCGT CTCACCCATA TCCGAAGCTG TGGAGGTGAA GGACTCTGCC AAACAGCCCC 841 AGATGGTGTT CACGGTCCGC CACGCCACCG TCACCTTCCA GCCAGAAGGG GACACGTGGC 901 GGGAGCAGAA GGAGCAGCGG GCCGCCCTCA TGGTGGGCAT CCTCATTGGC GTGTTCGTGC 961 TCTGCTGGAT CCCCTTCTTT CTCACCGAGC TCATCAGTCC CCTCTGCTCC TGTGACATCC 1021 CCGCCATCTG GAAAAGCATC TTCCTGTGGC TTGGCTACTC CAACTCCTTC TTTAACCCCC 1081 TGATCTATAC GGCTTTCAAC AAGAACTACA ACAGCGCCTT CAAGAACTTC TTTTCTAGGC 1141 AACACGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1201 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1261 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGACTAAGT CGTAGTTTTA TGTAAACGCG 1321 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt