Construct: ORF TRCN0000489887

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019411.1_s317c1
DNA Barcode:
GAACTTTCCGTTATGTCTCAATCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHRM3 (1131)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489887
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1131 CHRM3 cholinergic receptor muscar... NM_000740.3 99.9% 99.8% 1770_1771insG
2 human 1131 CHRM3 cholinergic receptor muscar... NM_001347716.2 99.9% 99.8% 1770_1771insG
3 human 1131 CHRM3 cholinergic receptor muscar... XM_005273032.3 99.9% 99.8% 1770_1771insG
4 human 1131 CHRM3 cholinergic receptor muscar... XM_011544041.2 99.9% 99.8% 1770_1771insG
5 human 1131 CHRM3 cholinergic receptor muscar... XM_011544043.2 99.9% 99.8% 1770_1771insG
6 human 1131 CHRM3 cholinergic receptor muscar... XM_011544044.2 99.9% 99.8% 1770_1771insG
7 human 1131 CHRM3 cholinergic receptor muscar... XM_011544046.2 99.9% 99.8% 1770_1771insG
8 human 1131 CHRM3 cholinergic receptor muscar... XM_011544047.2 99.9% 99.8% 1770_1771insG
9 human 1131 CHRM3 cholinergic receptor muscar... XM_017000152.2 99.9% 99.8% 1770_1771insG
10 human 1131 CHRM3 cholinergic receptor muscar... XM_017000153.1 99.9% 99.8% 1770_1771insG
11 human 1131 CHRM3 cholinergic receptor muscar... XM_017000154.1 99.9% 99.8% 1770_1771insG
12 human 1131 CHRM3 cholinergic receptor muscar... XM_017000155.1 99.9% 99.8% 1770_1771insG
13 human 1131 CHRM3 cholinergic receptor muscar... XM_017000156.1 99.9% 99.8% 1770_1771insG
14 human 1131 CHRM3 cholinergic receptor muscar... XM_017000157.2 99.9% 99.8% 1770_1771insG
15 human 1131 CHRM3 cholinergic receptor muscar... XM_017000158.1 99.9% 99.8% 1770_1771insG
16 human 1131 CHRM3 cholinergic receptor muscar... XM_017000159.1 99.9% 99.8% 1770_1771insG
17 human 1131 CHRM3 cholinergic receptor muscar... XM_017000160.2 99.9% 99.8% 1770_1771insG
18 human 1131 CHRM3 cholinergic receptor muscar... XM_017000161.1 99.9% 99.8% 1770_1771insG
19 human 1131 CHRM3 cholinergic receptor muscar... XM_017000162.1 99.9% 99.8% 1770_1771insG
20 human 1131 CHRM3 cholinergic receptor muscar... XM_017000163.2 99.9% 99.8% 1770_1771insG
21 mouse 12671 Chrm3 cholinergic receptor, musca... NM_033269.4 86.9% 91.5% (many diffs)
22 mouse 12671 Chrm3 cholinergic receptor, musca... XM_006516468.3 86.9% 91.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
1848
ORF length:
1773
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatgacc ttgcacaata acagtacaac ctcgcctttg tttccaaaca
121tcagctcctc ctggatacac agcccctccg atgcagggct gcccccggga accgtcactc
181atttcggcag ctacaatgtt tctcgagcag ctggcaattt ctcctctcca gacggtacca
241ccgatgaccc tctgggaggt cataccgtct ggcaagtggt cttcatcgct ttcttaacgg
301gcatcctggc cttggtgacc atcatcggca acatcctggt aattgtgtca tttaaggtca
361acaagcagct gaagacggtc aacaactact tcctcttaag cctggcctgt gccgatctga
421ttatcggggt catttcaatg aatctgttta cgacctacat catcatgaat cgatgggcct
481tagggaactt ggcctgtgac ctctggcttg ccattgacta cgtagccagc aatgcctctg
541ttatgaatct tctggtcatc agctttgaca gatacttttc catcacgagg ccgctcacgt
601accgagccaa acgaacaaca aagagagccg gtgtgatgat cggtctggct tgggtcatct
661cctttgtcct ttgggctcct gccatcttgt tctggcaata ctttgttgga aagagaactg
721tgcctccggg agagtgcttc attcagttcc tcagtgagcc caccattact tttggcacag
781ccatcgctgc tttttatatg cctgtcacca ttatgactat tttatactgg aggatctata
841aggaaactga aaagcgtacc aaagagcttg ctggcctgca agcctctggg acagaggcag
901agacagaaaa ctttgtccac cccacgggca gttctcgaag ctgcagcagt tacgaacttc
961aacagcaaag catgaaacgc tccaacagga ggaagtatgg ccgctgccac ttctggttca
1021caaccaagag ctggaaaccc agctccgagc agatggacca agaccacagc agcagtgaca
1081gttggaacaa caatgatgct gctgcctccc tggagaactc cgcctcctcc gacgaggagg
1141acattggctc cgagacgaga gccatctact ccatcgtgct caagcttccg ggtcacagca
1201ccatcctcaa ctccaccaag ttaccctcat cggacaacct gcaggtgcct gaggaggagc
1261tggggatggt ggacttggag aggaaagccg acaagctgca ggcccagaag agcgtggacg
1321atggaggcag ttttccaaaa agcttctcca agcttcccat ccagctagag tcagccgtgg
1381acacagctaa gacttctgac gtcaactcct cagtgggtaa gagcacggcc actctacctc
1441tgtccttcaa ggaagccact ctggccaaga ggtttgctct gaagaccaga agtcagaTCA
1501CTAAGCGGAA AAGGATGTCC CTGGTCAAGG AGAAGAAAGC GGCCCAGACC CTCAGTGCGA
1561TCTTGCTTGC CTTCATCATC ACTTGGACCC CATACAACAT CATGGTTCTG GTGAACACCT
1621TTTGTGACAG CTGCATACCC AAAACCTTTT GGAATCTGGG CTACTGGCTG TGCTACATCA
1681ACAGCACCGT GAACCCCGTG TGCTATGCTC TGTGCAACAA AACATTCAGA ACCACTTTCA
1741AGATGCTGCT GCTGTGCCAG TGTGACAAAA AAAAGAGGCG CAAGCAGCAG TACCAGCAGA
1801GACAGTCGGT CATTTTTCAC AAGCGCGCAC CCGAGCAGGC CTTGGACCCA GCTTTCTTGT
1861ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1921AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1981GAAAGGACGA GAACTTTCCG TTATGTCTCA ATCAACGCGT TAAGTCgaca atcaacctct
2041ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)