Construct: ORF TRCN0000489888
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019447.1_s317c1
- DNA Barcode:
- CTCGCTGCGCCGGTAGTAAGGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPAR2 (9170)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489888
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | NM_004720.6 | 99.9% | 100% | 714C>T |
| 2 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | XM_017027468.2 | 99.8% | 100% | 714C>T;864C>A |
| 3 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | XM_017027469.1 | 99.8% | 100% | 714C>T;864C>A |
| 4 | human | 9170 | LPAR2 | lysophosphatidic acid recep... | XM_024451774.1 | 99.8% | 100% | 714C>T;864C>A |
| 5 | mouse | 53978 | Lpar2 | lysophosphatidic acid recep... | NM_020028.3 | 85.7% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1128
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggtc atcatgggcc agtgctacta caacgagacc atcggcttct 121 tctataacaa cagtggcaaa gagctcagct cccactggcg gcccaaggat gtggtcgtgg 181 tggcactggg gctgaccgtc agcgtgctgg tgctgctgac caatctgctg gtcatagcag 241 ccatcgcctc caaccgccgc ttccaccagc ccatctacta cctgctcggc aatctggccg 301 cggctgacct cttcgcgggc gtggcctacc tcttcctcat gttccacact ggtccccgca 361 cagcccgact ttcacttgag ggctggttcc tgcggcaggg cttgctggac acaagcctca 421 ctgcgtcggt ggccacactg ctggccatcg ccgtggagcg gcaccgcagt gtgatggccg 481 tgcagctgca cagccgcctg ccccgtggcc gcgtggtcat gctcattgtg ggcgtgtggg 541 tggctgccct gggcctgggg ctgctgcctg cccactcctg gcactgcctc tgtgccctgg 601 accgctgctc acgcatggca cccctgctca gccgctccta tttggccgtc tgggctctgt 661 cgagcctgct tgtcttcctg ctcatggtgg ctgtgtacac ccgcattttc ttctacgtgc 721 ggcggcgagt gcagcgcatG GCAGAGCATG TCAGCTGCCA CCCCCGCTAC CGAGAGACCA 781 CGCTCAGTCT GGTCAAGACT GTTGTCATCA TCCTGGGGGC GTTCGTGGTC TGCTGGACAC 841 CAGGCCAGGT GGTACTGCTC CTGGATGGTT TAGGCTGTGA GTCCTGCAAT GTCCTGGCTG 901 TAGAAAAGTA CTTCCTACTG TTGGCCGAGG CCAACTCACT GGTCAATGCT GCTGTGTACT 961 CTTGCCGAGA TGCTGAGATG CGCCGCACCT TCCGCCGCCT TCTCTGCTGC GCGTGCCTCC 1021 GCCAGTCCAC CCGCGAGTCT GTCCACTATA CATCCTCTGC CCAGGGAGGT GCCAGCACTC 1081 GCATCATGCT TCCCGAGAAC GGCCACCCAC TGATGGACTC CACCCTTAAC CCAGCTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGACTCGCTG CGCCGGTAGT AAGGGCTACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt