Construct: ORF TRCN0000489900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019121.1_s317c1
- DNA Barcode:
- AAAATATTGGTATGATACTCCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA2 (1969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1969 | EPHA2 | EPH receptor A2 | NM_004431.5 | 99.9% | 99.7% | 2876C>T;2928_2929insG |
| 2 | human | 1969 | EPHA2 | EPH receptor A2 | NM_001329090.2 | 94.4% | 94.2% | 0_1ins162;2714C>T;2766_2767insG |
| 3 | human | 1969 | EPHA2 | EPH receptor A2 | XM_017000537.1 | 58.3% | 57.5% | (many diffs) |
| 4 | mouse | 13836 | Epha2 | Eph receptor A2 | NM_010139.3 | 86.5% | 92.5% | (many diffs) |
| 5 | mouse | 13836 | Epha2 | Eph receptor A2 | XM_006538528.3 | 62.6% | 67.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3003
- ORF length:
- 2931
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagctc caggcagccc gcgcctgctt cgccctgctg tggggctgtg 121 cgctggccgc ggccgcggcg gcgcagggca aggaagtggt actgctggac tttgctgcag 181 ctggagggga gctcggctgg ctcacacacc cgtatggcaa agggtgggac ctgatgcaga 241 acatcatgaa tgacatgccg atctacatgt actccgtgtg caacgtgatg tctggcgacc 301 aggacaactg gctccgcacc aactgggtgt accgaggaga ggctgagcgt atcttcattg 361 agctcaagtt tactgtacgt gactgcaaca gcttccctgg tggcgccagc tcctgcaagg 421 agactttcaa cctctactat gccgagtcgg acctggacta cggcaccaac ttccagaagc 481 gcctgttcac caagattgac accattgcgc ccgatgagat caccgtcagc agcgacttcg 541 aggcacgcca cgtgaagctg aacgtggagg agcgctccgt ggggccgctc acccgcaaag 601 gcttctacct ggccttccag gatatcggtg cctgtgtggc gctgctctcc gtccgtgtct 661 actacaagaa gtgccccgag ctgctgcagg gcctggccca cttccctgag accatcgccg 721 gctctgatgc accttccctg gccactgtgg ccggcacctg tgtggaccat gccgtggtgc 781 caccgggggg tgaagagccc cgtatgcact gtgcagtgga tggcgagtgg ctggtgccca 841 ttgggcagtg cctgtgccag gcaggctacg agaaggtgga ggatgcctgc caggcctgct 901 cgcctggatt ttttaagttt gaggcatctg agagcccctg cttggagtgc cctgagcaca 961 cgctgccatc ccctgagggt gccacctcct gcgagtgtga ggaaggcttc ttccgggcac 1021 ctcaggaccc agcgtcgatg ccttgcacac gacccccctc cgccccacac tacctcacag 1081 ccgtgggcat gggtgccaag gtggagctgc gctggacgcc ccctcaggac agcgggggcc 1141 gcgaggacat tgtctacagc gtcacctgcg aacagtgctg gcccgagtct ggggaatgcg 1201 ggccgtgtga ggccagtgtg cgctactcgg agcctcctca cggactgacc cgcaccagtg 1261 tgacagtgag cgacctggag ccccacatga actacacctt caccgtggag gcccgcaatg 1321 gcgtctcagg cctggtaacc agccgcagct tccgtactgc cagtgtcagc atcaaccaga 1381 cagagccccc caaggtgagg ctggagggcc gcagcaccac ctcgcttagc gtctcctgga 1441 gcatcccccc gccgcagcag agccgagtgt ggaagtacga ggtcacttac cgcaagaagg 1501 gagactccaa cagctacaat gtgcgccgca ccgagggttt ctccgtgacc ctggacgacc 1561 tggccccaga caccacctac ctggtccagg tgcaggcact gacgcaggag ggccaggggg 1621 ccggcagcaa ggtgcacgaa ttccagacgc tgtccccgga gggatctggc aacttggcgg 1681 tgattggcgg cgtggctgtc ggtgtggtcc tgcttctggt gctggcagga gttggcttct 1741 ttatccaccg caggaggaag aaccagcgtg cccgccagtc cccggaggac gtttacttct 1801 ccaagtcaga acaactgaag cccctgaaga catacgtgga cccccacaca tatgaggacc 1861 ccaaccaggc tgtgttgaag ttcactaccg agatccatcc atcctgtgtc actcggcaga 1921 aggtgatcgg agcaggagag tttggggagg tgtacaaggg catgctgaag acatcctcgg 1981 ggaagaagga ggtgccggtg gccatcaaga cgctgaaagc cggctacaca gagaagcagc 2041 gagtggactt cctcggcgag gccggcatca tgggccagtt cagccaccac aacatcatcc 2101 gcctagaggg cgtcatctcc aaatacaagc ccatgatgat catcactgag tacatggaga 2161 atggggccct ggacaagttc cttcgggaga aggatggcga gttcagcgtg ctgcagctgg 2221 tgggcatgct gcggggcatc gcagctggca tgaagtacct ggccaacatg aactatgtgc 2281 accgtgacct ggctgcccgc aacatcctcg tcaacagcaa cctggtctgc aaggtgtctg 2341 actttggcct gtcccgcgtg ctggaggacg accccgaggc cacctacacc accagtggcg 2401 gcaagatccc catccgctgg accgccccgg aggccatttc ctaccggaag ttcacctctg 2461 ccagcgacgt gtggagcttt ggcattgtca tgtgggaggt gatgacctat ggcgagcggc 2521 cctactggga gttgtccaac cacgaggtga tgaaagccat caatgatggc ttccggctcc 2581 ccacacccat ggactgcccc TCCGCCATCT ACCAGCTCAT GATGCAGTGC TGGCAGCAGG 2641 AGCGTGCCCG CCGCCCCAAG TTCGCTGACA TCGTCAGCAT CCTGGACAAG CTCATTCGTG 2701 CCCCTGACTC CCTCAAGACC CTGGCTGACT TTGACCCCCG CGTGTCTATC CGGCTCCCCA 2761 GCACGAGCGG CTCGGAGGGG GTGCCCTTCC GCACGGTGTC CGAGTGGCTG GAGTCCATCA 2821 AGATGCAGCA GTATACGGAG CACTTCATGG CGGCCGGCTA CACTGCCATC GAGAAGGTGG 2881 TGCAGATGAC CAACGACGAC ATCAAGAGGA TTGGGGTGCG GCTGCCCGGC CACCAGAAGC 2941 GCATCGTCTA CAGCCTGCTG GGACTCAAGG ACCAGGTGAA CACTGTGGGG ATCCCCATCG 3001 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3061 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3121 TTTATATATC TTGTGGAAAG GACGAAAAAT ATTGGTATGA TACTCCATAA CGCGTTAAGT 3181 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt